Sam68 regulates cell proliferation and cell adhesion‐mediated drug resistance (<scp>CAM</scp>‐<scp>DR</scp>) <i>via</i> the <scp>AKT</scp> pathway in non‐Hodgkin's lymphoma

Wu, Yong; Xu, Xiaohong; Miao, Xiaobing; Zhu, Xinghua; Yin, Haibing; He, Yunhua; Li, Chunsun; Liu, Yushan; Chen, Yali; Lu, Xiaoyun; Wang, Yuchan; He, Song

doi:10.1111/cpr.12220

Cited by 11 publications

(9 citation statements)

References 28 publications

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“…Based on the active metabolism characteristics of CSF-DLBCs, CNS-DLBCL might be grouped into the OxPhos-DLBCL cluster with downregulated BCR signaling pathway, especially in patients P1, P2, and P3 ( Figures 2 A and S3 ). As is well known, adhesive cell-cell and cell-matrix interactions generally play important roles in tumor metastases and drug resistance ( Wu et al., 2015 ). Whether the general downregulated cell adhesion molecule pathway ( Figures 2 A and S3 ) in CSF-DLBCs affects CNS-DLBCL metastases and drug resistance deserves further study.…”

Section: Discussionmentioning

confidence: 99%

Single-cell transcriptome analysis of diffuse large B cells in cerebrospinal fluid of central nervous system lymphoma

et al. 2021

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Summary Diffuse large B cells in the cerebrospinal fluid (CSF-DLBCs) have offered great promise for the diagnostics and therapeutics of central nervous system lymphoma (CNSL) leptomeningeal involvement. To explore the phenotypic states of CSF-DLBCs, we analyzed the transcriptomes of more than one thousand CSF-DLBCs from six patients with CNSL diffuse large B-cell lymphoma (DLBCL) using Smart-seq2 single-cell RNA sequencing. CSF-DLBCs were defined based on abundant expression of B-cell markers, the active cell proliferation and energy metabolism properties, and immunoglobulin light chain restriction. We identified inherent heterogeneity of CSF-DLBCs, which were mainly manifested in cell cycle state, cancer-testis antigen expression, and classification based on single-cell germinal center B-cell signature. In addition, the 16 upregulated genes in CSF-DLBCs compared to various normal B cells showed great possibility in the homing effect of the CNS-DLBCL for the leptomeninges. Our results will provide insight into the mechanism research and diagnostic direction of CNSL-DLBCL leptomeningeal involvement.

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Section: Discussionmentioning

confidence: 99%

Single-cell transcriptome analysis of diffuse large B cells in cerebrospinal fluid of central nervous system lymphoma

et al. 2021

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“…To elucidate the possible mechanism involved in cell proliferation, a number of important moleculars associated with tumorigenesis were detected. AKT and STAT3 participate in the growth and anti-apoptotic property of cancer cells 22 - 24 . It is valuable to discover whether TPPP3 influences the signaling cascade of the AKT and STAT3 pathways, which are involved in cell apoptosis and the cell cycle 25 , 26 .…”

Section: Discussionmentioning

confidence: 99%

Knockdown of Tubulin Polymerization Promoting Protein Family Member 3 Suppresses Proliferation and Induces Apoptosis in Non-Small-Cell Lung Cancer

Li¹,

Xu²,

Ye³

et al. 2016

J. Cancer

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Our previous studies demonstrated that depletion of tubulin polymerization promoting protein family member 3 (TPPP3) inhibits proliferation and induces apoptosis of HeLa cells. However, the expression and roles of TPPP3 in cancers remain largely unknown. In this study, we investigated the expression of TPPP3 in clinicopathological correlations in non-small-cell lung cancer (NSCLC) samples by immunohistochemistry. TPPP3 expression was significantly upregulated in NSCLC tissues, and high TPPP3 expression was positively associated with tumor size, lymph node metastasis, clinical stage, and poor survival. Furthermore, knockdown of TPPP3 by shRNA significantly inhibited cell proliferation and induced cell apoptosis and cell cycle arrest in vitro. In addition, depletion of TPPP3 inhibited lung cancer growth in vivo in the xenografts of H1299 cells; this effect was accompanied by the suppression of Ki67 expression. Our data suggested that TPPP3 might act as an oncogene in NSCLC. TPPP3 warrants consideration as a therapeutic candidate with anti-tumor potential.

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“…Based on the active metabolism characteristics of CSF-DLBCs, CNS-DLBCL might be grouped into the OxPhos-DLBCL cluster with down-regulated B cell receptor signaling pathway, especially patients P1, P2 and P3 (Figure 2A; supplemental Figure 4). As is well known, adhesive cell-cell and cell-matrix interactions generally play important roles in tumor metastases and drug resistance 40 . Whether the generally down-regulated cell adhesion molecules (CAMs) pathway in CSF-DLBCs affects CNS-DLBCL metastases and drug resistance deserves further study.…”

Section: Discussionmentioning

confidence: 99%

Single cell transcriptomic analysis of diffuse large B cells in cerebrospinal fluid of central nervous system lymphoma

Ruan

Wang

Zhai

et al. 2020

Preprint

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Diffuse large B-cell lymphoma (DLBCL) is the predominant type of central nervous system lymphoma (CNSL) including primary CNSL and secondary CNSL. Diffuse large B cells in cerebrospinal fluid (CSF-DLBCs) have offered great promise for the diagnostics and therapeutics of CNSL leptomeningeal involvement. To explore the distinct phenotypic states of CSF-DLBCs, we analyzed the transcriptomes of 902 CSF-DLBCs from six CNSL-DLBCL patients using single-cell RNA sequencing technology. We defined CSF-DLBCs based on abundant expression of B-cell markers, as well as the enrichment of cell proliferation and energy metabolism pathways. CSF-DLBCs within individual patients exhibited monoclonality with similar variable region of lightchains (VL) expression. It is noteworthy that we observed some CSF-DLBCs have double classes of VL (lambda and kappa) transcripts. We identified substantial heterogeneity in CSF-DLBCs, and found significantly greater among-patient heterogeneity compared to among-cell heterogeneity within a given patient. The transcriptional heterogeneity across CSF-DLBCs is manifested in cell cycle state and cancer-testis antigens expression. Our results will provide insight into the mechanism research and new diagnostic direction of CNSL-DLBCL leptomeningeal involvement.

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Sam68 regulates cell proliferation and cell adhesion‐mediated drug resistance (CAM‐DR) via the AKT pathway in non‐Hodgkin's lymphoma

Abstract: Increased Sam68 expression in NHL resulted in poor prognosis, and it promoted CAM-DR in NHL via AKT.

Cited by 11 publications

References 28 publications

Single-cell transcriptome analysis of diffuse large B cells in cerebrospinal fluid of central nervous system lymphoma

Single-cell transcriptome analysis of diffuse large B cells in cerebrospinal fluid of central nervous system lymphoma

Knockdown of Tubulin Polymerization Promoting Protein Family Member 3 Suppresses Proliferation and Induces Apoptosis in Non-Small-Cell Lung Cancer

Single cell transcriptomic analysis of diffuse large B cells in cerebrospinal fluid of central nervous system lymphoma

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