Sambar, an Indian dish prevents the development of dimethyl hydrazine-induced colon cancer: A preclinical study

Prasad, V. Ganga; Reddy, Neetinkumar D.; Francis, Albi; Nayak, Pawan G.; Kishore, Anoop; Nandakumar, Krishnadas; Rao, Mallikarjuna C; Shenoy, Rekha R

doi:10.4103/0973-1296.191454

Cited by 8 publications

(4 citation statements)

References 19 publications

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“…Spices are frequently used as part of functional foods, for example, the Indian dish rasam is a concoction of different spices and has been reported to be hypoglycemic, anti-anemic and antipyretic [ 49 ]. Sambar , another predominantly spice-based recipe has been shown to work against prostrate cancer [ 50 ]. Traditional medicinal systems are also known to recommend spices as part of their prescriptions.…”

Section: Discussionmentioning

confidence: 99%

Data-driven analysis of biomedical literature suggests broad-spectrum benefits of culinary herbs and spices

et al. 2018

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Spices and herbs are key dietary ingredients used across cultures worldwide. Beyond their use as flavoring and coloring agents, the popularity of these aromatic plant products in culinary preparations has been attributed to their antimicrobial properties. Last few decades have witnessed an exponential growth of biomedical literature investigating the impact of spices and herbs on health, presenting an opportunity to mine for patterns from empirical evidence. Systematic investigation of empirical evidence to enumerate the health consequences of culinary herbs and spices can provide valuable insights into their therapeutic utility. We implemented a text mining protocol to assess the health impact of spices by assimilating, both, their positive and negative effects. We conclude that spices show broad-spectrum benevolence across a range of disease categories in contrast to negative effects that are comparatively narrow-spectrum. We also implement a strategy for disease-specific culinary recommendations of spices based on their therapeutic tradeoff against adverse effects. Further by integrating spice-phytochemical-disease associations, we identify bioactive spice phytochemicals potentially involved in their therapeutic effects. Our study provides a systems perspective on health effects of culinary spices and herbs with applications for dietary recommendations as well as identification of phytochemicals potentially involved in underlying molecular mechanisms.

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Section: Discussionmentioning

confidence: 99%

Data-driven analysis of biomedical literature suggests broad-spectrum benefits of culinary herbs and spices

et al. 2018

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“…Before each dosing, the DMH solution was freshly prepared. The carcinogen was injected intraperitoneally (i.p) once a week for the first 10 weeks at a dosage of 20 mg/kg and 30 mg/kg for the next 10 weeks during the 20-week induction period [ 16 , 17 ].…”

Section: Methodsmentioning

confidence: 99%

“…Following carcinogen induction, group III received the standard drug 5-Flurouracil (10 mg/kg, i.p) once a week, while, groups IV and V received AR leaf extract 5 mg/kg and 10 mg/kg, p.o. daily respectively for 21 days [ 16 , 17 ].…”

Section: Methodsmentioning

confidence: 99%

Unveiling antioxidant and anti-cancer potentials of characterized Annona reticulata leaf extract in 1,2-dimethylhydrazine-induced colorectal cancer in Wistar rats

Kumar

et al. 2021

Journal of Ayurveda and Integrative Medicine

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Background Colorectal cancer (CC) is the third most common cancer in the world. Annona reticulata (AR) also known as bullock's heart, is a traditional herb. AR leaf extract was initially investigated for its anti-bacterial, anti-inflammatory, anti-malarial, anti-helminthic, anti-stress, and wound healing properties. Only a few in vitro cancer studies have been conducted on AR. Although few studies have linked AR leaf extract to many cancers, comprehensive studies addressing regulation, biological functions, and molecular mechanisms leading to CC pathogenesis are clearly lacking. Objectives The present study aimed to explore the antioxidant and anti-cancer potentials of AR leaf extract in CC. Materials and methods The MTT assay was used to test the anti-proliferative activity of AR leaf extract in vitro on the HCT116 cell line. Qualitative and quantitative phytochemical characterization was carried out using gas chromatography: mass spectrometry (GC–MS). 1,2-dimethylhydrazine (DMH) was used to establish CC model in female Wistar rats. The acute toxicity of AR leaf extract was tested in accordance with OECD guidelines. Aberrant Crypt Foci (ACF) count, organ index, and hematological estimations were used to screen for in vivo anti-cancer potential. The antioxidant activity of colon homogenate was determined. Results The alcoholic leaf extract (IC50, 0.55 μg/ml) was found to be more potent than the aqueous extract. Using GC–MS, a total of 108 compounds were quantified in the alcoholic leaf extract. The LD 50 value was found to be safe at a dose of 98.11 mg/kg of body weight. AR alcoholic leaf extract significantly (p < 0.05) decreased ACF count and normalized colon length/weight ratio. AR leaf extract increased RBC, hemoglobin and platelets levels. The AR alcoholic leaf extract reduced the DMH-induced tumors and significantly (p < 0.05) increased the activity of endogenous antioxidant enzymes such as catalase, reduced glutathione, superoxide dismutase, and decreased the lipid peroxidase activity. AR leaf extract reduced the inflammation caused by DMH and helped to repair the colon's damaged muscle layers. Conclusion Based on the findings from the present study, it can be concluded that the alcoholic leaf extract of AR has antioxidant and anti-proliferative properties and can aid in the prevention of CC development and dysplasia caused by DMH.

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“…Sambar , another South Indian traditional dish, has shown preventive effect against colon cancer. [ 8 ] There is a need to understand traditional systems and visualize the future of medicine and health care. The linkage between “the past” and “the future” of medicine is much more important and can give us “new directions” for better understanding health, disease, and possible solutions.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic, antimitotic, and antiproliferation studies on Rasam: A South Indian traditional functional food

Devarajan

Raja

2017

Phcog Mag

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Background:Rasam is a traditional South Indian food, prepared using tamarind juice as a base, with a variety of spices. Rasam, with all its ingredients medicinally claimed for various ailments, is a functional food. Systematic consumption of traditional functional food provides an excellent preventive measure to ward off many diseases.Objective:To study rasam for cytotoxic, antimitotic, and antiproliferation potential beyond its culinary and nutritional effect.Materials and Methods:Brine shrimp lethality assay, onion root tip inhibition assay, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay in Calu-6, HeLa, MCF-7 cell lines for four stage-wise samples in the preparation of rasam (RS1, RS2, RS3, and RS4) were studied.Results:RS4, the end product of rasam showed high lethality with an LC50 value of 38.7 μL/mL. It showed maximum antimitotic activity in a dose-dependent manner compared to other samples with an IC50 value of 189.86 μL/mL. RS4 also showed an IC50 value of 350.22 and 410.15 μL/mL in MCF-7 and Calu-6 cell lines, respectively.Conclusion:From this study, we suggest that rasam is a classic example of traditional functional food and it can treat breast and lung cancer on chronic use.SUMMARY Rasam, a South Indian traditional functional food, showed high lethality (LC50 = 38.7 mL/mL) against brine shrimpsRasam also showed potential antimitotic activity (IC50 = 189.86 mL/mL) by inhibiting the onion root tipsRasam showed an IC50 value of 350.22 and 410.15 mL/mL against MCF-7 and Calu-6 cell lines respectivelyRasam, when consumed on daily dietary basis, can treat breast and lung cancer. Abbreviations used: SS 316: Stainless Steel 316 grade; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; DMEM: Dulbecco's modified Eagle medium; FBS: Fetal bovine serum media; TPVG: Trypsin phosphate versene glucose; EDTA: Ethylene diamine tetra acetic acid; PBS: Phosphate buffered saline; DMSO: Dimethyl sulfoxide.

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Sambar, an Indian dish prevents the development of dimethyl hydrazine-induced colon cancer: A preclinical study

Cited by 8 publications

References 19 publications

Data-driven analysis of biomedical literature suggests broad-spectrum benefits of culinary herbs and spices

Data-driven analysis of biomedical literature suggests broad-spectrum benefits of culinary herbs and spices

Unveiling antioxidant and anti-cancer potentials of characterized Annona reticulata leaf extract in 1,2-dimethylhydrazine-induced colorectal cancer in Wistar rats

Cytotoxic, antimitotic, and antiproliferation studies on Rasam: A South Indian traditional functional food

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