Unveiling antioxidant and anti-cancer potentials of characterized Annona reticulata leaf extract in 1,2-dimethylhydrazine-induced colorectal cancer in Wistar rats

Abstract

Background Colorectal cancer (CC) is the third most common cancer in the world. Annona reticulata (AR) also known as bullock's heart, is a traditional herb. AR leaf extract was initially investigated for its anti-bacterial, anti-inflammatory, anti-malarial, anti-helminthic, anti-stress, and wound healing properties. Only a few in vitro cancer studies have been conducted on AR. Although few studies have linked AR leaf extract to many cancers, comprehensive studies addressing regulation, biological functions, and molecular mechanisms leading to CC pathogenesis are clearly lacking. Objectives The present study aimed to explore the antioxidant and anti-cancer potentials of AR leaf extract in CC. Materials and methods The MTT assay was used to test the anti-proliferative activity of AR leaf extract in vitro on the HCT116 cell line. Qualitative and quantitative phytochemical characterization was carried out using gas chromatography: mass spectrometry (GC–MS). 1,2-dimethylhydrazine (DMH) was used to establish CC model in female Wistar rats. The acute toxicity of AR leaf extract was tested in accordance with OECD guidelines. Aberrant Crypt Foci (ACF) count, organ index, and hematological estimations were used to screen for in vivo anti-cancer potential. The antioxidant activity of colon homogenate was determined. Results The alcoholic leaf extract (IC50, 0.55 μg/ml) was found to be more potent than the aqueous extract. Using GC–MS, a total of 108 compounds were quantified in the alcoholic leaf extract. The LD 50 value was found to be safe at a dose of 98.11 mg/kg of body weight. AR alcoholic leaf extract significantly (p < 0.05) decreased ACF count and normalized colon length/weight ratio. AR leaf extract increased RBC, hemoglobin and platelets levels. The AR alcoholic leaf extract reduced the DMH-induced tumors and significantly (p < 0.05) increased the activity of endogenous antioxidant enzymes such as catalase, reduced glutathione, superoxide dismutase, and decreased the lipid peroxidase activity. AR leaf extract reduced the inflammation caused by DMH and helped to repair the colon's damaged muscle layers. Conclusion Based on the findings from the present study, it can be concluded that the alcoholic leaf extract of AR has antioxidant and anti-proliferative properties and can aid in the prevention of CC development and dysplasia caused by DMH.

Protective Effect of Ethanol Extract of Annona Muricata Leaves on Hematological Profile, Histology and Oxidative Stress in 1,2-Dimethylhydrazine-induced Colorectal Carcinogenesis in Rats

“…In this study, there was no mortality and also from the results, a significant decrease in change in body weight of the animals treated with the carcinogen (DMH) was observed. Loss of weight is one of the indices associated with cancer (Nadeem et al, 2021). 1,2-dimethylhydrazine-induced colon cancer is associated with significant production of oxygen free radicals leading to increased lipid peroxidation, which causes damage to cells and leading to carcinogenesis (Crosara et al, 2014).…”

Comprehensive Tools of Alkaloid/Volatile Compounds–Metabolomics and DNA Profiles: Bioassay-Role-Guided Differentiation Process of Six Annona sp. Grown in Egypt as Anticancer Therapy

“…Moreover, A. reticulata has been observed to be potentially employed as a chemopreventive agent in cancer therapy. Furthermore, A. muricata is a well-known medicinal remedy in Africa, America, and India for cancer treatment [ 16 ]. A. squamosa and A. atemoya are employed in the treatment of tumors [ 17 ].…”

Plant secondary metabolites for the prevention and treatment of colorectal cancer: A review

“…The gastrointestinal problems, urinary incontinence, sexual dysfunction and sensory neuropathy were also observed on the long-term treatment using chemotherapeutic agents and others [8,9] . Thus, there is a need to design and develop a new class of compounds with less or without toxicity along with high efficiency to replace the conventional synthetic agents.…”