Sample preparation techniques coupled to advanced chromatographic methods for marine organisms investigation

Costa, Rosaria; Beccaria, Marco; Grasso, Elisa; Albergamo, Ambrogina; Oteri, Marianna; Dugo, Paola; Fasulo, Salvatore; Mondello, Luigi

doi:10.1016/j.aca.2015.03.032

Cited by 27 publications

(21 citation statements)

References 28 publications

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“…The bidimensional plots obtained highlighted, in real time, differences between samples having different origin. This report follows a previous one based on the development of extraction methodologies suitable for the isolation of lipids from the same marine species here investigated .…”

Section: Introductionmentioning

confidence: 82%

Multidimensional gas chromatographic techniques applied to the analysis of lipids from wild‐caught and farmed marine species

Costa

Albergamo

Piparo

et al. 2016

Euro J Lipid Sci & Tech

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Comprehensive two-dimensional gas chromatography-mass spectrometry was exploited for the obtainment of fatty acid fingerprints in fish and molluscs from South Italy. Scope of the lipidomic investigation was to highlight differences in the fatty acid profiles of samples from aquaculture and from the wild. The fish species under investigation were Sparus aurata and Dicentrarchus labrax, typically cultivated in Italy and commonly found in the fish market. Another aim was to find a correlation between the diet fed to fish and the expression of specific fatty acids in muscle composition. Beyond fish, two species of bivalves were analyzed to assess their lipidomic composition: Mytilus galloprovincialis (mussels) and Venerupis aurea var. laeta (clams). Both species originated from two salt lakes in South Italy. GC Â GC-MS analysis showed that omega-3 fatty acids were naturally more concentrated in wild fish, whereas their presence in cultivated fish was lower and in favor of omega-6 and oleic acids, as an evident consequence of food supplementation. Bivalves reported a consistent amount of PUFA, although presenting some differences in the quality of fatty acids. As an additional parameter of investigation, the content of mercury was determined in all the samples by means of a Direct Mercury Analyzer, based on Atomic Absorption Spectroscopy. Wild fish reported a level of mercury that was ten times higher than in aquafarmed fish, and the maximum amount tolerated by the EC regulation. On the contrary, the level of mercury in molluscs was in general very low, particularly when compared with samples from other Italian sites of collection.Practical applications: One of the possible applications of the GC Â GC methodology here presented could be the detection of a common food fraud. In the Mediterranean Sea, gilthead sea bream and sea bass are today rarely found in sea open waters. Basically, fish sold in the market comes from cultivation. Due to the fact that wild fish is more appreciated by the consumer, quite often fish sellers are encouraged to lie about the real origin of fish. The bidimensional plots shown in this study represent an extraordinary tool for differentiating the two types of fish. Also, this study provides substantial support to aquafarmers, who can draw useful advice on how to tune optimal diets to be fed to fish. In fact, it was demonstrated that fish feed highly affects fatty acid composition.

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Section: Introductionmentioning

confidence: 82%

Multidimensional gas chromatographic techniques applied to the analysis of lipids from wild‐caught and farmed marine species

Costa

Albergamo

Piparo

et al. 2016

Euro J Lipid Sci & Tech

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“…The potential of off-line LC Â LC coupling of nonaqueous RP and silver-ion chromatography was successfully demonstrated in three different works Costa et al, 2015). In all set-ups, the approach investigated provided the highest separation capacity of TAGs in complex natural samples of plant oils and marine fats resulting in the highest number of identified TAGs ever reported for studied samples.…”

Section: Lc â Lc Of Natural Products 293mentioning

confidence: 92%

“…On the one hand, for NP Â RP separation of carotenoids, either silica or cyanopropyl (CN) stationary phases have been investigated (Dugo et al , 2008d(Dugo et al ,e, 2009cCacciola et al, 2012Cacciola et al, , 2016a, whereas for triacylglycerol (TAG) separation, an Ag Â RP combination turned out to be useful for this task Dugo et al, 2006b,c;Van der Klift et al, 2008;Hu et al, 2013;Wei et al, , 2015Costa et al, 2015;. On the other hand, for RP Â RP separation of phenolic antioxidants, the effect of several stationary phase chemistries (C8, C18, CN, Diol, F 5 , NH 2 , PEG, Phenyl, RPAmide, and other laboratory-made stationary phases) on the separation selectivity and resolution has been investigated (Blahovà et al, 2006;Cacciola et al, 2006Cacciola et al, , 2007aCacciola et al, ,b,c, 2011Hyilompolo, 2007, 2008;Pol et al, 2007, Pol andDugo et al, 2008cDugo et al, , 2009aJandera et al, 2008aJandera et al, ,b, 2012Hájek et al, 2008Cesla et al, 2009;Kalili and de Villiers, 2009, 2013aZhou et al, 2009;Russo et al, 2011;Fu et al, 2012;Hájek and Jandera, 2012;Kalili et al 2013Kalili et al , 2014Montero et al, 2013aMontero et al, ,b, 2014Montero et al, , 2016aKrol-Kogus et al, 2014;Krauze-Baranowska et al, 2014;Leme et al, 2014;Tanaka et al, 2014;…”

Section: Lc 3 Lc Of Natural Productsmentioning

confidence: 99%

Theory and Practice of UHPLC and UHPLC–MS

Guillarme

Veuthey

2017

Handbook of Advanced Chromatography /Mass Spectrometry Techniques

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“…phospholipids) and nonpolar intact lipids (e.g. TAGs and cholesterol ester) into FA methyl esters, TAGs require LC separations coupled with MS detection for the comprehensive characterization [19]. Furthermore, if possible, UHPLC methods are employed due to the high complexity of these samples.…”

Section: Introductionmentioning

confidence: 99%

Lipid profile of fish species by liquid chromatography coupled to mass spectrometry and a novel linear retention index database

Rigano

Oteri

Micalizzi

et al. 2020

J of Separation Science

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In a previous article, Rigano et al. established a new linear retention index system for the identification of triacylglycerols by liquid chromatography methods only on the basis of the retention behavior and independently from many experimental parameters. In that work, a database of 209 compounds was built, but only 54 of them, typical of vegetable oils, were confirmed by mass spectrometry. The aim of the present research is to extend the applicability of the novel approach to more complex samples, such as fish lipid extracts, and assess the complementarity between mass spectromtery and retention information to achieve univocal identification. With this purpose, a new software was implemented to make the identification process easy and automatic as in gas chromatography-mass spectrometry where the retention index filter is added in the spectral search to discriminate between compounds with similar mass spectrometry spectra. A total of 69 species were identified and, thanks to their baseline separation obtained by an ultra high performance liquid chromatography method, a semiquantification was also performed. The species under investigation were Dicentrarchus labrax, coming from aquaculture and the wild. Some differences in their native lipid composition were observed, probably related to a different diet. A major number of samples would be necessary to confirm such a preliminary finding. K E Y W O R D Slinear retention index, lipids, sea bass, spectral library, triacylglycerols

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Sample preparation techniques coupled to advanced chromatographic methods for marine organisms investigation

Cited by 27 publications

References 28 publications

Multidimensional gas chromatographic techniques applied to the analysis of lipids from wild‐caught and farmed marine species

Multidimensional gas chromatographic techniques applied to the analysis of lipids from wild‐caught and farmed marine species

Theory and Practice of UHPLC and UHPLC–MS

Lipid profile of fish species by liquid chromatography coupled to mass spectrometry and a novel linear retention index database

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