Sample-stacking techniques in non-aqueous capillary electrophoresis

Tsai, Chih Hsin; Tsai, Chung Chen; Liu, Ju Tsung; Lin, Cheng Huang

doi:10.1016/j.chroma.2004.12.095

Cited by 23 publications

(18 citation statements)

References 37 publications

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“…In an interesting approach to performing FASS, Tsai et al [26] have examined two ways of introducing a high conductivity zone to enhance the conductivity difference with the sample and improve preconcentration. In the first approach, they used a conventional hydrodynamic injection of an ultra-high conductivity zone (36 higher than the electrolyte) prior to sample injection while in the second approach, a low-temperature bath was used to cool 4-8 cm of the capillary to 2157C.…”

Section: Llementioning

confidence: 99%

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips

2007

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Poor sensitivity is considered to be one of the major limitations of electrophoretic separation methods, particularly when compared to traditional liquid chromatographic techniques. To address this issue, various in-line preconcentration techniques have been developed over the past 15 years, ranging in power and complexity, and there are now a number of well understood approaches routinely capable of providing a 10,000- to 100,000-fold increase in sensitivity, as well as several that can be pushed above a million. Furthermore, these have been achieved with particularly troublesome and often difficult samples, such as those having high salinity from a biological or environmental origin. This review will discuss the most common methods for improving the sensitivity of CE, CEC and microchip version of these, with particular attention to those approaches developed over the last five years.

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Section: Llementioning

confidence: 99%

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips

2007

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“…The LOQs were improved by 8-400-fold by the FASS-LVI technique. Tsai et al [21,22] developed ultra-high conductivity zone NACE-stacking (UHCZ/NACE-stacking) and low-temperature-zone NACE-stacking (LTZ/NACEstacking) methods. By comparing UHCZ/NACE-stacking and LTZ/NACE-stacking with FASS-LVI, the sensitivity of the former two methods was about 6-fold higher than that of FASS-LVI.…”

Section: Introductionmentioning

confidence: 99%

NACE‐ESI‐MS combined with on‐line concentration for high‐sensitivity analysis of quinolizidine alkaloids

Wang

Cheng

2007

Electrophoresis

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A rapid, reproducible and high-sensitivity NACE-ESI-MS method was developed for the analysis of sophoridine, matrine, sophocarpine and oxymatrine in the roots of Sophora flavescens Ait. and S. tonkinensis Gagnep. Field-amplified sample stacking with electromigration-injection (FASS-EMI) was first used in NACE for the on-line concentration of the alkaloids. The conditions of NACE separation, FASS-EMI stacking and MS detection were systematically optimized. The optimum NACE buffer was an electrolyte containing 50 mM ammonium acetate, 0.5% acetic acid and 30% ACN in methanol. The sensitivity was improved by about 100-fold by the FASS-EMI technique, which was further improved by more than 1000-fold with MS detection. The RSDs (n = 6) of the relative migration time and relative peak area of each peak were less than 0.3 and 2.4% for intra-day and less than 5.1 and 6.0% for inter-day, respectively. The LODs (S/N = 3) of analytes were determined to be 0.0210-0.0446 ng/mL. A bioanalytical method based on this NACE-ESI-MS method may be developed for the analysis of the alkaloids in biological sample matrices (plasma, urine, etc.) after effective ion removal.

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“…FASS, already evaluated by Zhang and Thormann [23] has been proposed by Tsai et al [24]. The authors reported an improved sample-stacking technique based on the introduction of an ultra-high conductivity zone between the sample zone and the background solution.…”

Section: Forensic Drugs Toxicants and Dyesmentioning

confidence: 98%

Recent advances in the applications of CE to forensic sciences (2005–2007)

Tagliaro

Bortolotti

2007

Electrophoresis

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The present article reviews the applications of CE in forensic science covering the period from 2005 until the first part of 2007. The overview includes the most relevant examples of analytical applications of capillary electrophoretic and electrokinetic techniques in the following fields: (i) forensic drugs, toxicants and dyes, (ii) small ions of forensic interest (iii) explosives, (iv) forensic DNA, and (v) other biopolymers of forensic interest.

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Sample-stacking techniques in non-aqueous capillary electrophoresis

Cited by 23 publications

References 37 publications

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips

NACE‐ESI‐MS combined with on‐line concentration for high‐sensitivity analysis of quinolizidine alkaloids

Recent advances in the applications of CE to forensic sciences (2005–2007)

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