“…10 In humans, one similar study has been conducted, testing the effect of tracking on the absolute values of myocardial velocity variables at three locations in the heart. 27 This study showed that tracking of the SV resulted in a significantly reduced e 0 velocity in all investigated segments compared with end-systolic sampling, in contrast to our data in which no difference could be demonstrated. This study also demonstrated that manual tracking reduced intraobserver, but not Interobserver, variability, which is in agreement with our results.…”