In this paper, moisture content effects on horizontal flame spread were experimentally investigated using 0.245 mm thick, 28 cm tall and 28 cm wide untreated cotton fabric sheets with various moisture contents varying from 0 to 34%. The pyrolysis spread rates, flame heights and ignition times were obtained and analyzed. The corresponding results are as follows: as moisture content increases, the flame height and spread rate first increase and then decrease. In contrast, the ignition time shows an opposite trend with moisture content. The extreme values are observed in cases of 2% moisture content samples. Moreover, the flame spread rate in the warp direction is larger than that in the weft direction. For horizontal flame spread, the moisture content has the effect of consuming part of the heat feedback, which can play a role in reducing the flame spread rate; simultaneously, the moisture content can enlarge flame size and increase the convective heat transfer coefficient, thereby resulting in an increase in flame spread rate. The non-monotonous trend in pyrolysis spread rate is the result of competition between these effects.