Sampling of Candida albicans and Candida tropicalis by Langerin-positive dendritic cells in mouse Peyer’s patches

Jesus, Magdia De; Rodriguez, Adam E.; Yagita, Hideo; Ostroff, Gary R.; Mantis, Nicholas J.

doi:10.1016/j.imlet.2015.09.008

Cited by 22 publications

(28 citation statements)

References 52 publications

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“…We found that C. albicans strains ATCC18804 and SC5314, as well as C. tropicalis , were able to cross the intestinal epithelial barrier, and that uptake was partly dependent on M-cells. These findings were supported by Albac et al, who demonstrated C. albicans strain SC5314 uses M-cells as a portal of entry into the intestinal barrier (16, 17). C. albicans was able to preferentially invade M-cells via actin-mediated endocytosis rather than active penetration (16, 17).…”

Section: Introductionsupporting

confidence: 54%

“…These findings were supported by Albac et al, who demonstrated C. albicans strain SC5314 uses M-cells as a portal of entry into the intestinal barrier (16, 17). C. albicans was able to preferentially invade M-cells via actin-mediated endocytosis rather than active penetration (16, 17). In addition to its ability to cross the epithelial barrier, we also found that C. albicans was sampled by a subset of SED CD11c + phagocytes that expressed the C-type lectin Langerin (16).…”

Section: Introductionsupporting

confidence: 54%

“…M-cells are important in the controlled transcytosis of luminal antigens and microbes. We and others previously reported that C. albicans ’ translocation into PPs was partially M-cell dependent (16, 17, 27-30). It has been shown that the Rorc deficiency plays a role in susceptibility to Candida sp.…”

Section: Resultsmentioning

confidence: 78%

“…Earlier studies by Bonifazi et al showed that PP dendritic cells (DCs) had functional plasticity and played a role in both inflammation and tolerance in the presence of C. albicans in vivo . Interestingly, the immune response that was elicited depended on whether C. albicans was in a yeast or hyphal form (16, 18). C. albicans in the yeast form promoted the activation of Rorc + 17-producing CD4 + T cells and Gata3 + IL-4/IL-10-producing CD4 + T cells with minimal activation of tbet + IFN-γ-producing cells, an immune response that resulted in a limited control of fungal growth (16, 18).…”

Section: Introductionmentioning

confidence: 99%

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Setting off the Alarms:Candida albicansElicits Pro-Inflammatory Differential Gene Expression in Intestinal Peyer’s Patches

Singh

Song

et al. 2019

Preprint

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24Candida albicans has been associated with a number of human diseases that pertain to 25 the gastrointestinal (GI) tract. However, the details of how gut-associated lymphoid tissues 26 (GALT) such as Peyer's patches (PPs) in the small intestine play a role in immune surveillance 27 and microbial differentiation, and what mechanisms PP use to protect the mucosal barrier in 28 response to fungal organisms such as C. albicans, are still unclear. We particularly focus on PPs 29 as they are the immune sensors and inductive sites of the gut that influence inflammation and 30 tolerance. We have previously demonstrated that CD11c + phagocytes located in the sub-31 epithelial dome (SED) within PPs sample C. albicans. To gain insight on how specific cells 32within PPs sense and respond to the sampling of fungi, we gavaged mice with C. albicans strains 33 ATCC 18804 and SC5314 as well as Saccharomyces cerevisiae. We measured the differential 34 gene expression of sorted CD45 + B220 + B-cells, CD3 + T-cells, and CD11c + DCs within the first 35 24 hrs post-gavage using nanostring nCounter® technology. The results reveal that at 24 hrs, PP 36 phagocytes were the cell type that displayed differential gene expression. These phagocytes were 37 both able to sample C. albicans and able to discriminate between strains. In particular, strain 38 ATCC 18804 upregulated fungal specific pro-inflammatory genes in CD11c + phagocytes 39 pertaining to innate and adaptive immune responses. Interestingly, PP CD11c + phagocytes 40 differentially expressed genes in response to C. albicans that were important in the protection of 41 the mucosal barrier. These results highlight that the mucosal barrier not only responds to C. 42 albicans, but also aids in the protection of the host. 43 Importance 44The specific gene expression changes within PPs that send the warning signals when 45 encountering fungi, and how PPs can discriminate between innocuous S. cerevisiae or different 46 strains of C. albicans during early stages of sampling, have not been elucidated. Here we show 47 that within the first 24 hours of sampling, CD11c + phagocytes were not only important in 48 sampling, but they were the cell type that exhibited clear differential gene expression. These 49 differentially expressed genes play important dual roles in inflammation, chemotaxis, and fungal 50 specific recognition, as well as maintaining homeostasis and protection of the mucosal barrier. 51Using nanostring technology, we were also able to demonstrate that PPs can distinguish between 52 different strains of C. albicans and can "set off the alarms" when necessary. 53 54

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Section: Introductionsupporting

confidence: 54%

Section: Introductionsupporting

confidence: 54%

Section: Resultsmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Setting off the Alarms:Candida albicansElicits Pro-Inflammatory Differential Gene Expression in Intestinal Peyer’s Patches

Singh

Song

et al. 2019

Preprint

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“…During this passage, it will encounter mucosal cells lining the GI tract, soluble host molecules, and a large variety of cells of the microbiome. In addition, as other food constituents, C. utilis cells (intact or partially degraded) or its components may adhere to M cells in the small intestine and become translocated to antigenpresenting cells of Peyer's plaques or other lymphoid tissue associated with the GI tract (De Jesus et al 2015;Thévenot et al 2015). To prevent harmful inflammatory reactions in response to food, immunological tolerance is the natural outcome to most food antigens presented at the GI mucosa (Feng and Elson 2011).…”

Section: Utilis Medical Uses: Oral Tolerance and Beyondmentioning

confidence: 99%

Candida utilis and Cyberlindnera (Pichia) jadinii: yeast relatives with expanding applications

Buerth

Tielker

Ernst

2016

Appl Microbiol Biotechnol

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The yeast Candida utilis is used as a food additive and as a host for heterologous gene expression to produce various metabolites and proteins. Reliable protocols for intracellular production of recombinant proteins are available for C. utilis and have now been expanded to secrete proteins into the growth medium or to achieve surface display by linkage to a cell wall protein. A recombinant C. utilis strain was recently shown to induce oral tolerance in a mouse model of multiple sclerosis suggesting future applications in autoimmune therapy. Whole genome sequencing of C. utilis and its presumed parent Cyberlindnera (Pichia) jadinii demonstrated different ploidy but high sequence identity, consistent with identical recombinant technologies for both yeasts. C. jadinii was recently described as an antagonist to the important human fungal pathogen Candida albicans suggesting its use as a probiotic agent. The review summarizes the status of recombinant protein production in C. utilis, as well as current and future biotechnological and medical applications of C. utilis and C. jadinii.

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Impact of the Environment upon the Candida albicans Cell Wall and Resultant Effects upon Immune Surveillance

Childers

Avelar

Bain

et al. 2019

Current Topics in Microbiology and Immunology

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The fungal cell wall is an essential organelle that maintains cellular morphology and protects the fungus from environmental insults. For fungal pathogens such as Candida albicans, it provides a degree of protection against attack by host immune defences. However, the cell wall also presents key epitopes that trigger host immunity, and attractive targets for antifungal drugs. Rather than being a rigid shield, it has become clear that the fungal cell wall is an elastic organelle that permits rapid changes in cell volume and the transit of large liposomal particles such as extracellular vesicles. The fungal cell wall is also flexible in that it adapts to local environmental inputs, thereby enhancing the fitness of the fungus in these microenvironments. Recent evidence indicates that this cell wall adaptation affects hostfungus interactions by altering the exposure of major cell wall epitopes that are recognised by innate immune cells. Therefore, we discuss the impact of environmental adaptation upon fungal cell wall structure and immune evasion, focussing on C. albicans and drawing parallels with other fungal pathogens.

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Sampling of Candida albicans and Candida tropicalis by Langerin-positive dendritic cells in mouse Peyer’s patches

Cited by 22 publications

References 52 publications

Setting off the Alarms:Candida albicansElicits Pro-Inflammatory Differential Gene Expression in Intestinal Peyer’s Patches

Setting off the Alarms:Candida albicansElicits Pro-Inflammatory Differential Gene Expression in Intestinal Peyer’s Patches

Candida utilis and Cyberlindnera (Pichia) jadinii: yeast relatives with expanding applications

Impact of the Environment upon the Candida albicans Cell Wall and Resultant Effects upon Immune Surveillance

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