Sampling soil microfloras: Dispersion of soil by ion exchange and extraction of specific microorganisms from suspension by elutriation

Macdonald, R. M.

doi:10.1016/0038-0717(86)90045-3

Cited by 77 publications

(25 citation statements)

References 14 publications

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“…Bakken [3] did not find a significant dispersion difference between hexametaphosphate and distilled water. Many other chemical agents have been evaluated: specific detergents (sodium cholate and sodium deoxycholate), which interact with bacterial lipopolysaccharides [40], polyethylene glycol (PEG) and SDS which dissolve hydrophobic material [63], PVPP which removes humic acids [63]. However, chemical agents may have adverse side effects.…”

Section: Soil Dispersion Methodsmentioning

confidence: 99%

Extraction of DNA from soil

Robe¹,

Nalin²,

Capellano³

et al. 2003

European Journal of Soil Biology

254

164

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Section: Soil Dispersion Methodsmentioning

confidence: 99%

Extraction of DNA from soil

Robe¹,

Nalin²,

Capellano³

et al. 2003

European Journal of Soil Biology

254

164

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“…To obtain viable cells, the soil sample can be converted into a suspension, agitated by vortexing or sonication and the supernatant spread on to solid media to obtain single colonies. Alternatively, the cells can be removed from the soil by using chemical dispersants to dissociate the micro-organisms from soil particles (for example, MacDonald, 1986;Hopkins et al, 1991 a, 6 ) in single-or multi-stage procedures followed by either the isolation of single colonies or an estimation of biomass.…”

Section: Sampling Strategymentioning

confidence: 99%

Development of molecular methods for the detection of specific bacteria in the environment

Pickup¹

1991

Journal of General Microbiology

116

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“…Dispersion is brought about by mechanical means such as shaking, blending, ultrasonic treatment (at energy levels that do not disintegrate bacteria) (Ramsey, 1984;Bakken, 1985;Faegri et al, 1977;MacDonald, 1986). Dislodgement may be helped by addition of detergents, e.g.…”

Section: Dispersion and Dislodgementmentioning

confidence: 99%

“…Dislodgement may be helped by addition of detergents, e.g. sodium deoxycholate and the use of ion exchange resins (Hopkins and O'Donnell, 1992;Jacobsen and Rasmussen, 1992;MacDonald, 1986). The applicant may be able to report on their own experience with specific methods; e.g.…”

Section: Dispersion and Dislodgementmentioning

confidence: 99%

Safety Assessment of Transgenic Organisms, Volume 4

2010

Harmonisation of Regulatory Oversight in Biotechnology

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io s a fe t y nv ir on me nt ag ri cu lt ur e bi os af et y g r ic u lt u r e e n vi r o n m e n t gr ic ul tu re bi os af et y en vi ro nm en t n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o io sa fe ty en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en v io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t nv ir on me nt ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm e g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a f gr ic ul tu re bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t a n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u io sa fe ty en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m nv ir on me nt ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a f gr ic ul tu re bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t a n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u io sa fe ty en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e nv ir on me nt ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y e g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a f ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et...

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Sampling soil microfloras: Dispersion of soil by ion exchange and extraction of specific microorganisms from suspension by elutriation

Cited by 77 publications

References 14 publications

Extraction of DNA from soil

Extraction of DNA from soil

Development of molecular methods for the detection of specific bacteria in the environment

Safety Assessment of Transgenic Organisms, Volume 4

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