Sandwich Immunoassays of Multicomponent Subtrace Pathogenic DNA Based on Magnetic Fluorescent Encoded Nanoparticles

Liu, Yaxu; Zhang, Xuanjun; Yifeng, E; Fang, Fang; Kuang, Guangkai; Wang, Guannan

doi:10.1155/2016/7324384

Cited by 5 publications

(5 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Goldcoated magnetic nanoparticles improve effective bacteria (e.g., E. coli) separation and rapid quantification (Torun et al, 2012). Amino function MNPs extract ultra-low-level of viruses which can then be detected by immunosensors and chemiluminescent sensors (Liu et al, 2016. The immobilization of enzymes on MNPs also allows the recovery of enzyme via magnetic field, thus achieves the reuse of biosensors up to 32 times (Othman et al, 2016, Teepoo andLaochai, 2021).…”

Section: Othersmentioning

confidence: 99%

Progress of optical biosensors for analyzing pathogens and organic pollutants in water since 2015

et al. 2022

View full text Add to dashboard Cite

Water contamination by pathogens and organic pollutants is one of the major environmental problems that risk human health. Climate change with extreme weather can promote their prevalence in waters. Environmental monitoring of these pollutants in a fast, continuous, and accurate manner is of increasing demand, especially under the climate change context, but is challenged by their ubiquity and trace concentrations. Optical biosensing is one of the desired solutions owing to its rapid and accurate detection with high sensitivity. Principally, an optical biosensor recognizes these bioactive toxins and contaminants by tailored bioreceptors (e.g., aptamer, enzyme, and cells) and transduces the biological response to optical signals. Research efforts have been made on tailoring bioreceptors and enhancing signal transducing by nanoparticles. This study comprehensively reviewed the mechanisms of optical biosensing and the recent development of bioreceptors and nanomaterials on the enhancement for the rapid, easy, and accurate analysis of emerging contaminants in water. The advantages and challenges on sensitivity, selectivity, and durability of biosensors were discussed along with the opportunities and development strategies.

show abstract

Section: Othersmentioning

confidence: 99%

Progress of optical biosensors for analyzing pathogens and organic pollutants in water since 2015

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Taking immunoreactions into an account, typical immunosensors can work on several assay-modes. (i.e., competitive, direct, sandwich or indirect modes) [250][251][252][253]. Such assay formats work on the unique mechanisms and usually entail the events mentioned below [11,79]:…”

Section: Electrochemical Gqd Sensors In Biomedical Diagnosticsmentioning

confidence: 99%

Applications of Graphene Quantum Dots in Biomedical Sensors

Mansuriya

Altıntaş

2020

Sensors

190

View full text Add to dashboard Cite

Due to the proliferative cancer rates, cardiovascular diseases, neurodegenerative disorders, autoimmune diseases and a plethora of infections across the globe, it is essential to introduce strategies that can rapidly and specifically detect the ultralow concentrations of relevant biomarkers, pathogens, toxins and pharmaceuticals in biological matrices. Considering these pathophysiologies, various research works have become necessary to fabricate biosensors for their early diagnosis and treatment, using nanomaterials like quantum dots (QDs). These nanomaterials effectively ameliorate the sensor performance with respect to their reproducibility, selectivity as well as sensitivity. In particular, graphene quantum dots (GQDs), which are ideally graphene fragments of nanometer size, constitute discrete features such as acting as attractive fluorophores and excellent electro-catalysts owing to their photo-stability, water-solubility, biocompatibility, non-toxicity and lucrativeness that make them favorable candidates for a wide range of novel biomedical applications. Herein, we reviewed about 300 biomedical studies reported over the last five years which entail the state of art as well as some pioneering ideas with respect to the prominent role of GQDs, especially in the development of optical, electrochemical and photoelectrochemical biosensors. Additionally, we outline the ideal properties of GQDs, their eclectic methods of synthesis, and the general principle behind several biosensing techniques.

show abstract

“…However, in an indirect immunoassay, a labeled secondary antibody is subjected to a specific primary antibody [72,79]. In the case of a sandwich assay, antigens are "sandwiched" between the capture and detection antibodies through two different binding sites [11,80].…”

Section: Electrochemical Sensorsmentioning

confidence: 99%

Graphene Quantum Dot-Based Electrochemical Immunosensors for Biomedical Applications

Mansuriya

Altıntaş

2019

Materials

View full text Add to dashboard Cite

In the area of biomedicine, research for designing electrochemical sensors has evolved over the past decade, since it is crucial to selectively quantify biomarkers or pathogens in clinical samples for the efficacious diagnosis and/or treatment of various diseases. To fulfil the demand of rapid, specific, economic, and easy detection of such biomolecules in ultralow amounts, numerous nanomaterials have been explored to effectively enhance the sensitivity, selectivity, and reproducibility of immunosensors. Graphene quantum dots (GQDs) have garnered tremendous attention in immunosensor development, owing to their special attributes such as large surface area, excellent biocompatibility, quantum confinement, edge effects, and abundant sites for chemical modification. Besides these distinct features, GQDs acquire peroxidase (POD)-mimicking electro-catalytic activity, and hence, they can replace horseradish peroxidase (HRP)-based systems to conduct facile, quick, and inexpensive label-free immunoassays. The chief motive of this review article is to summarize and focus on the recent advances in GQD-based electrochemical immunosensors for the early and rapid detection of cancer, cardiovascular disorders, and pathogenic diseases. Moreover, the underlying principles of electrochemical immunosensing techniques are also highlighted. These GQD immunosensors are ubiquitous in biomedical diagnosis and conducive for miniaturization, encouraging low-cost disease diagnostics in developing nations using point-of-care testing (POCT) and similar allusive techniques.

show abstract

Sandwich Immunoassays of Multicomponent Subtrace Pathogenic DNA Based on Magnetic Fluorescent Encoded Nanoparticles

Cited by 5 publications

References 32 publications

Progress of optical biosensors for analyzing pathogens and organic pollutants in water since 2015

Progress of optical biosensors for analyzing pathogens and organic pollutants in water since 2015

Applications of Graphene Quantum Dots in Biomedical Sensors

Graphene Quantum Dot-Based Electrochemical Immunosensors for Biomedical Applications

Contact Info

Product

Resources

About