Sanguinarine enhances cisplatin sensitivity via glutathione depletion in cisplatin‐resistant ovarian cancer (A2780) cells

Sarkhosh-Inanlou, Roya; Molaparast, Morteza; Adel, Milad; Shafiei‐Irannejad, Vahid

doi:10.1111/cbdd.13621

Cited by 41 publications

(16 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Remarkable phosphorylation of H2AX, cleaved PARP, caspase activation and downregulation of survivin and STAT3 in combinational treatment, as compared to alone drugs, provides the molecular basis of SNG-mediated sensitization of PTC cells to cisplatin. In this context, earlier reports also provide additional support for the combinational therapeutic potential of SNG [48,49], indicating that SNG has the potential to affect the growth and proliferation of cancer cells alone and can sensitize malignant cells to cancer therapeutic drugs.…”

Section: Discussionmentioning

confidence: 81%

See 1 more Smart Citation

Sanguinarine Induces Apoptosis in Papillary Thyroid Cancer Cells via Generation of Reactive Oxygen Species

et al. 2020

View full text Add to dashboard Cite

Sanguinarine (SNG), a natural compound with an array of pharmacological activities, has promising therapeutic potential against a number of pathological conditions, including malignancies. In the present study, we have investigated the antiproliferative potential of SNG against two well-characterized papillary thyroid cancer (PTC) cell lines, BCPAP and TPC-1. SNG significantly inhibited cell proliferation of PTC cells in a dose and time-dependent manner. Western blot analysis revealed that SNG markedly attenuated deregulated expression of p-STAT3, without affecting total STAT3, and inhibited growth of PTC via activation of apoptotic and autophagy signaling cascade, as SNG treatment of PTC cells led to the activation of caspase-3 and caspase-8; cleavage of PARP and activation of autophagy markers. Further, SNG-mediated anticancer effects in PTC cells involved the generation of reactive oxygen species (ROS) as N-acetyl cysteine (NAC), an inhibitor of ROS, prevented SNG-mediated antiproliferative, apoptosis and autophagy inducing action. Interestingly, SNG also sensitized PTC cells to chemotherapeutic drug cisplatin, which was inhibited by NAC. Finally, SNG suppressed the growth of PTC thyrospheres and downregulated stemness markers ALDH2 and SOX2. Altogether, the findings of the current study suggest that SNG has anticancer potential against PTC cells as well its derived cancer stem-like cells, most likely via inactivation of STAT3 and its associated signaling molecules.

show abstract

Section: Discussionmentioning

confidence: 81%

“…Reactive oxygen species (ROS) production, among others, has been considered as a prime underlying mechanism for SNG [30,46,47]. Furthermore, SNG has been found to sensitize cancer cells to anticancer drugs through attenuated resistance and stemness [35,48,49].…”

Section: Introductionmentioning

confidence: 99%

Sanguinarine Induces Apoptosis in Papillary Thyroid Cancer Cells via Generation of Reactive Oxygen Species

et al. 2020

View full text Add to dashboard Cite

show abstract

“…This method includes the cyclocondensation of 3-nitrobenzaldehyde with two equivalents of ethyl acetoacetate in the presence of a nitrogen donor such as ammonia or ammonium acetate (based on the procedure reported in the literature). 33 We needed a quick entry into 1,4-dihydropyridine-3,5-dicarboxylic acid diesters in which the 2,6-methyl groups were altered by a range of different groups. Allylic bromination is the replacement of a hydrogen on a carbon adjacent to a double bond.…”

Section: Resultsmentioning

confidence: 99%

Synthesis and assessment of the cytotoxic effect of some of 1,4-dihydropyridine derivatives which contain azole moiety

2021

View full text Add to dashboard Cite

A number of 1,4-dihydropyridine derivatives (9a-d, 10a-d, and 11a-d) were designed and synthesized by the reaction of 1,3,4-oxadiazole-5-thiones and 1,2,4-triazole-5-thiones to 2,6-dibromomethyl-3,5-diethoxycarbonyl-4-(3-ni-trophenyl)-1,4-dihydropyridine. The synthesized compounds were characterrized using FT-IR, 1H-NMR, 13C-NMR spectral data, ESI-MS, and elemental analysis. The cytotoxicity of the synthesized compounds was evaluated in human breast cancer (MCF-7) cells based on the results of MTT assay. The results indicated that compound Diethyl 4-(3-nitrophenyl)-2,6-bis[((5-(3-nitrophenyl)-1,3,4-oxa-diazol-2-yl)thio)methyl]-1,4-dihydro pyridine-3,5-dicarboxylate (9b) with (IC50 = 23 ? 2.32 ?M) was the most potent derivative against MCF-7 cells. Based on the results, the use of oxadiazole moiety in the C2 and C6 positions of 1,4-di-hydropyridine ring system enhanced the cytotoxic potential of these derivatives. Therefore, some of the oxadiazole-substituted 1,4-DHPs may facilitate further modifications which result in the discovery of potent cytotoxic agents.

show abstract

“…Recent reports show that sanguinarine exhibits low toxicity 30 and promotes apoptosis in hepatocellular carcinoma 31 and is reported to treat cervical cancer 32 and as a potent adjuvant for ovarian cancer chemotherapy 33 . In addition, sanguinarine is a potential drug against trichinellosis, a zoonotic parasitic disease, 34 and it has exhibited antifungal properties 35 .…”

Section: Resultsmentioning

confidence: 99%

Efficient extraction and purification of benzo[c]phenanthridine alkaloids from Macleaya cordata (Willd) R. Br. by combination of ultrahigh pressure extraction and pH‐zone‐refining counter‐current chromatography with anti‐breast cancer activity in vitro

Ali

Cui

et al. 2020

Phytochemical Analysis

View full text Add to dashboard Cite

Introduction Macleaya cordata (Willd) R. Br. (Papaveraceae family) is a well‐known traditional Chinese medicine used to treat muscle pain, inflamed wounds, and bee bites. Benzo[c]phenanthridine alkaloids are the main active ingredients in M. cordata. In this work, sanguinarine and chelerythrine were efficiently extracted and purified by ultrahigh‐pressure extraction (UHPE) technique and pH‐zone‐refining counter‐current chromatography (PZRCCC) from M. cordata. Objective To develop an efficient UHPE method followed by an efficient separation technique using PZRCCC for benzo[c]phenanthridine alkaloids from the study plant species, and to evaluate the study samples for anti‐breast cancer activity. Methodology The optimal extraction conditions were optimised as extraction pressure 200 MPa, extraction solvent 95% ethanol, solid–liquid ratio 1:30 (g/mL) and extraction time 2 min. A two‐phase n‐hexane/ethyl acetate/i‐propanol/water (1:3:1.5:4.5, v/v) solvent system was optimised with 10 mmol triethylamine in the upper phase and 10 mmol trifluoroacetic acid in lower phase in PZRCCC. The sample loading was optimised as 2.50 g. Moreover, the samples were evaluated for anti‐breast cancer activity later on. Results The 2.50 g sample loading yielded 0.45 g of sanguinarine and 0.59 g chelerythrine in one‐step separation using PZRCCC. The anti‐breast cancer activities of sanguinarine and chelerythrine were found stronger than positive control (vincristine 5.04 μg/mL) with half‐maximal inhibitory concentration values of 0.96 and 3.00 μg/mL, respectively. Conclusion This study showed that the established methods were efficient in extraction (UHPE) and separation (PZRCCC) of the sanguinarine and chelerythrine from M. cordata.

show abstract

Sanguinarine enhances cisplatin sensitivity via glutathione depletion in cisplatin‐resistant ovarian cancer (A2780) cells

Cited by 41 publications

References 26 publications

Sanguinarine Induces Apoptosis in Papillary Thyroid Cancer Cells via Generation of Reactive Oxygen Species

Sanguinarine Induces Apoptosis in Papillary Thyroid Cancer Cells via Generation of Reactive Oxygen Species

Synthesis and assessment of the cytotoxic effect of some of 1,4-dihydropyridine derivatives which contain azole moiety

Efficient extraction and purification of benzo[c]phenanthridine alkaloids from Macleaya cordata (Willd) R. Br. by combination of ultrahigh pressure extraction and pH‐zone‐refining counter‐current chromatography with anti‐breast cancer activity in vitro

Contact Info

Product

Resources

About