Sanitary Selection and Molecular Characterization of Olive Cultivars Grown in Apulia

Montemurro, Cinzia; Saponari, María; Bottalico, Giovanna; Simeone, Rosanna; Savino, V.; Pasqualone, Antonella; Blanco, Antonio; Martelli, G. P.

doi:10.17660/actahortic.2008.791.93

Cited by 4 publications

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“…In the olive, microsatellite regions were sequenced in the early 2000s, and since the publication of specific primers [57,88,89], they have been increasingly used to dissect all aspects of a crop's genetic diversity [90]. More polymorphic than RAPD and AFLP [91], they have often been used in combination with other molecular markers for synergistic enhancement, in particular, to discriminate related olive genotypes [92][93][94].…”

Section: Ssr Markermentioning

confidence: 99%

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How to Choose a Good Marker to Analyze the Olive Germplasm (Olea europaea L.) and Derived Products

Sion

Savoia

Gadaleta

et al. 2021

Genes

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The olive tree (Olea europaea L.) is one of the most cultivated crops in the Mediterranean basin. Its economic importance is mainly due to the intense production of table olives and oil. Cultivated varieties are characterized by high morphological and genetic variability and present a large number of synonyms and homonyms. This necessitates the introduction of a rapid and accurate system for varietal identification. In the past, the recognition of olive cultivars was based solely on analysis of the morphological traits, however, these are highly influenced by environmental conditions. Therefore, over the years, several methods based on DNA analysis were developed, allowing a more accurate and reliable varietal identification. This review aims to investigate the evolving history of olive tree characterization approaches, starting from the earlier morphological methods to the latest technologies based on molecular markers, focusing on the main applications of each approach. Furthermore, we discuss the impact of the advent of next generation sequencing and the recent sequencing of the olive genome on the strategies used for the development of new molecular markers.

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Section: Ssr Markermentioning

confidence: 99%

“…The AFLP technique was optimised for fragmented DNA for oils traceability studies, overcoming the PCR's inhibition problems due to the presence of high amounts of phenolic compounds [94]. Nevertheless, the most used molecular marker in olive oil traceability is SSR [202].…”

Section: The Molecular Markers Used For Olive Oil Traceability Purposesmentioning

confidence: 99%

How to Choose a Good Marker to Analyze the Olive Germplasm (Olea europaea L.) and Derived Products

Sion

Savoia

Gadaleta

et al. 2021

Genes

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show abstract

“…The simultaneous use of AFLPs and microsatellites clarified the relationships among some Slovenian and Italian cultivars, as well as among Italian, Spanish, French and Greek cultivars, or within germplasm from the Emilia, Apulia and Campania regions of Italy. In addition, the combination of AFLP and RAPD markers allowed to establish the genetic relationships among Greek, Spanish, Italian, Algerian and Egyptian olive cultivars, and the combination of RAPDs and microsatellites was exploited in the examination of other cultivars from the Emilia region, as well as to genotype a reference collection of French olive germplasm, allowing the distinction of the majority of samples .…”

Section: Combined Use Of Different Dna Markersmentioning

confidence: 99%

Evolution and perspectives of cultivar identification and traceability from tree to oil and table olives by means of DNA markers

et al. 2016

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In recent years, an increasing number of typicality marks has been awarded to high-quality olive oils produced from local cultivars. In this case, quality control requires effective varietal checks of the starting materials. Moreover, accurate cultivar identification is essential in vegetative-propagated plants distributed by nurseries and is a pre-requisite to register new cultivars. Food genomics provides many tools for cultivar identification and traceability from tree to oil and table olives. The results of the application of different classes of DNA markers to olive with the purpose of checking cultivar identity and variability of plant material are extensively discussed in this review, with special regard to repeatability issues and polymorphism degree. The characterization of olive germplasm from all countries of the Mediterranean basin and from less studied geographical areas is described and innovative high-throughput molecular tools to manage reference collections are reviewed. Then the transferability of DNA markers to processed products - virgin olive oils and table olives - is overviewed to point out strengths and weaknesses, with special regard to (i) the influence of processing steps and storage time on the quantity and quality of residual DNA, (ii) recent advances to overcome the bottleneck of DNA extraction from processed products, (iii) factors affecting whole comparability of DNA profiles between fresh plant materials and end-products, (iv) drawbacks in the analysis of multi-cultivar versus single-cultivar end-products and (v) the potential of quantitative polymerase chain reaction (PCR)-based techniques. © 2016 Society of Chemical Industry.

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Electrotherapy; a promising therapy to eradicate viruses from infected in vitro olive cv. Meshkat

Mirzaei,

Yadollahi,

Naderpour

et al. 2024

Plant Cell Tiss Organ Cult

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Sanitary Selection and Molecular Characterization of Olive Cultivars Grown in Apulia

Cited by 4 publications

References 0 publications

How to Choose a Good Marker to Analyze the Olive Germplasm (Olea europaea L.) and Derived Products

How to Choose a Good Marker to Analyze the Olive Germplasm (Olea europaea L.) and Derived Products

Evolution and perspectives of cultivar identification and traceability from tree to oil and table olives by means of DNA markers

Electrotherapy; a promising therapy to eradicate viruses from infected in vitro olive cv. Meshkat

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