Santhica 23 et 27 : deux variétés de chanvre (Cannabis sativa L.) sans Δ-9-THC

Fournier, G.; Beherec, Olivier; Bertucelli, Sylvestre

doi:10.1051/ata/2004017

Cited by 10 publications

(11 citation statements)

References 17 publications

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“…It could also be an aid for checking the identity of cannabis specimen of different origin, next to other techniques as determination of microelements or stable isotopes of carbon and nitrogen [30]. In addition, quantification of total CBG can be useful for the identification of different types of fibre hemp analyzed [17].…”

Section: Resultsmentioning

confidence: 99%

“…Fedora 17 is a "classical" fibre-type variety containing CBDA and CBD as major cannabinoids. Santhica 27 is a new variety of hemp in which the biogenesis of cannabinoids seems to have stopped precociously: CBGA and CBG are the main cannabinoids [17]. Sample 11 is a non-psychotropic cannabis which grew wild (seeds coming from bird food).…”

Section: Discussionmentioning

confidence: 99%

“…in forensic chemistry [5,8,[14][15][16][17][18]. However, as this method is based on heating the sample, thermal conversion occurs and the acidic forms of cannabinoids are converted into their decarboxylated counterparts.…”

Section: Discussionmentioning

confidence: 99%

“…Gas Chromatography (GC) is the most commonly used method for the analysis of cannabis products [5,8,[14][15][16][17][18], but it does not permit the determination of acidic cannabinoids due to decarboxylation into their neutral forms during analysis. Furthermore, this thermal conversion of acidic cannabinoids seems to be incomplete [19].…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Innovative development and validation of an HPLC/DAD method for the qualitative and quantitative determination of major cannabinoids in cannabis plant material

Backer

Debrus

Lebrun

et al. 2009

Journal of Chromatography B

257

219

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GC is commonly used for the analysis of cannabis samples, e.g. in forensic chemistry. However, as this method is based on heating of the sample, acidic forms of cannabinoids are decarboxylated into their neutral counterparts. Conversely, HPLC permits the determination of the original composition of plant cannabinoids by direct analysis. Several HPLC methods have been described in the literature, but most of them failed to separate efficiently all the cannabinoids or were not validated according to general guidelines. By use of an innovative methodology for modelling chromatographic responses, a simple and accurate HPLC/DAD method was developed for the quantification of major neutral and acidic cannabinoids present in cannabis plant material: Delta9-tetrahydrocannabinol (THC), THC acid (THCA), cannabidiol (CBD), CBD acid (CBDA), cannabigerol (CBG), CBG acid (CBGA) and cannabinol (CBN). Delta8-Tetrahydrocannabinol (Delta8-THC) was determined qualitatively. Following the practice of design of experiments, predictive multilinear models were developed and used in order to find optimal chromatographic analytical conditions. The method was validated following an approach using accuracy profiles based on beta-expectation tolerance intervals for the total error measurement, and assessing the measurements uncertainty. This analytical method can be used for diverse applications, e.g. plant phenotype determination, evaluation of psychoactive potency and control of material quality.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Innovative development and validation of an HPLC/DAD method for the qualitative and quantitative determination of major cannabinoids in cannabis plant material

Backer

Debrus

Lebrun

et al. 2009

Journal of Chromatography B

257

219

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“…Le travail sur ce critère a ensuite conduit, en 2002, à l'inscription de la variété Santhica 27, variété dont la teneur en Δ9-THC est nulle (Fournier et al, 2004).…”

Section: La Teneur En δ9-tétrahydrocannabinol (δ9-thc)unclassified

La sélection française du chanvre : panorama et perspectives

Thouminot¹

2015

OCL

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Reçu le 22 juin 2015 -Accepté le 30 juillet 2015Résumé -Le chanvre industriel est cultivé sur des surfaces limitées (surfaces proches de 11 000 ha en France en 2014, source CETIOM), mais comme pour toute espèce cultivée, une activité de sélection est nécessaire. Celle-ci est conduite en France par un acteur unique depuis plusieurs dizaines d'années, la Fédération Nationale des Producteurs de Chanvre (FNPC). Cet article présente les différents critères de sélection actuels et à venir. Certains axes historiques sont toujours travaillés aujourd'hui, comme la teneur en Δ9-tétrahydrocannabinol, dans un souci de respect des réglementations, ou le maintien de l'état monoïque, permettant une culture mécanisée. D'autres critères de sélection ont évolué, en particulier la précocité variétale et le rendement paille. Pour ces deux critères, de nouveaux objectifs sont apparus : pour la précocité variétale, l'équipe de sélection cherche à élargir la gamme existante, quant au rendement paille, le but actuel est d'améliorer la productivité de la plante, c'est-à-dire d'augmenter le rendement à précocité constante. Enfin, de nouveaux axes de sélection apparaissent, comme par exemple la facilité de défibrage et la tolérance à l'orobanche. Mots clés :Chanvre industriel / création variétale / critères de sélection / Δ9-THC / fibres Abstract -Hemp breeding in France: overview and prospects. Industrial hemp cultivation surfaces are low compared to major crops (almost 11 000 ha in France for 2014, according to CETIOM), but, as all cultivated crops, a breeding activity is needed. Since several decades, this activity is conducted in France by a single structure, which is "Fédération Nationale des Producteurs de Chanvre" (FNPC). This article presents the main current and future breeding criteria. Some historical criteria are still relevant today such as Δ9-tetrahydrocannabinol level in order to comply with the regulations, and monoecy to allow crop mechanization. Others axis had evolved, for instance earliness and straw yield. There are new goals for these two criteria. For earliness, breeding is aiming at creating new varieties earlier and later than those existing and for straw yield, it is now targeted to improve yield at constant earliness (i.e. improve productivity). Finally, breeders begin to work on new purposes such as varieties with easy decortication or broomrape tolerance.

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Validating a predictive model of cannabinoid inheritance with feral, clinical, and industrial Cannabis sativa

Wenger

Dabney

ElSohly

et al. 2020

American J of Botany

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of America. This is an open access article under the terms of the Creat ive Commo ns Attri butio n-NonCo mmerc ial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. • 1423 Natural genetic variation affects the composition of phytochemicals in plants and can be of great economic importance. With a record of use by humans as food, fiber, and medicine spanning thousands of years (Faeti et al., 1996), recent decades of prohibition, and a rapidly changing regulatory context, Cannabis sativa L. (Cannabaceae) was until recently among the least studied agricultural crops (Small, 2016). Prominent among the traits of C. sativa are cannabinoids, a unique class of specialized metabolites synthesized and stored in glandular trichomes that are located on the floral bracts of pistillate inflorescences (Livingston et al., 2020; Fig. 1A-C). The ratio of the two most abundant cannabinoids, tetrahydrocannabinolic acid (THCA) and cannabidiolic acid (CBDA), hereafter THC and CBD, is represented by three main classes: THC-type plants with THC:CBD ≥ 10, intermediate-type plants with THC:CBD ≈ 1, and CBD-type plants with THC:CBD ≤ 0.1 (de Meijer et al., 2003). Some authors have referred to these chemotype (chemical phenotype) classes as Type I, Type II, and Type III (Small, 2016). Here we introduce for clarity a more descriptive classification of THC-type, intermediate-type, and CBD-type plants. Descriptive terms also avoid confusion associated with recent statutory definitions of C. sativa that vary widely among political jurisdictions (e.g., "industrial hemp" and "medical marijuana"). Our findings suggest that patterns of cannabinoid inheritance render some of these popular definitions inaccurate at least from a botanical perspective.

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Santhica 23 et 27 : deux variétés de chanvre (Cannabis sativa L.) sans Δ-9-THC

Abstract: RÉSUMÉLe cannabigérol (CBG) MOTS-CLÉS

Cited by 10 publications

References 17 publications

Innovative development and validation of an HPLC/DAD method for the qualitative and quantitative determination of major cannabinoids in cannabis plant material

Innovative development and validation of an HPLC/DAD method for the qualitative and quantitative determination of major cannabinoids in cannabis plant material

La sélection française du chanvre : panorama et perspectives

Validating a predictive model of cannabinoid inheritance with feral, clinical, and industrial Cannabis sativa

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