Sappanone A protects mice against cisplatin-induced kidney injury

Kang, Lin; Zhao, Hongfu; Chen, Chen; Zhang, Xiuzhi; Xu, Mingtang; Duan, Huijun

doi:10.1016/j.intimp.2016.05.019

Cited by 29 publications

(18 citation statements)

References 22 publications

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“…We must acknowledge that there existed some shortages in this present study. First, besides anti-apoptotic activity, SA also possessed antioxidant and anti-inflammatory activities based on previous reports [6,7]. However, we did not provide data on its antioxidant and anti-inflammatory effects on MIRI in the present study.…”

Section: Discussionmentioning

confidence: 60%

“…Sappanone A (SA) (chemical formula: C 16 H 12 O 5 ), 4H-1-Benzopyran-4-one,3-[(3,4-dihydroxyphenyl)methylene]-2,3-dihydro-7-hydroxy-(9CI), a kind of homoisoflavanone, is originally isolated from the heartwood of Caesalpinia sappan L. [5]. Recent studies have reported that SA could exert multiple pharmacological effects, including antioxidant [6], anti-inflammatory [7] and anti-apoptotic [8] activities. For instance, SA treatment increased the level of Nrf2 and HO-1, decreased cisplatin-induced TNF-α and IL-1β production, and repressed NF-κB activation in a dose-dependent manner in human proximal tubule epithelial cells [6].…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies have reported that SA could exert multiple pharmacological effects, including antioxidant [6], anti-inflammatory [7] and anti-apoptotic [8] activities. For instance, SA treatment increased the level of Nrf2 and HO-1, decreased cisplatin-induced TNF-α and IL-1β production, and repressed NF-κB activation in a dose-dependent manner in human proximal tubule epithelial cells [6]. More interesting, SA pretreatment protected PC-12 cells against hypoxia-induced damage through inhibiting apoptosis [8].…”

Section: Introductionmentioning

confidence: 99%

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Sappanone A alleviates hypoxia/reoxygenation-induced cardiomyocytes injury through inhibition of mitochondrial apoptosis and activation of PI3K–Akt–Gsk-3β pathway

Shi¹,

Gao²,

Ji³

et al. 2020

Bioscience Reports

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Myocardial ischemia reperfusion injury (MIRI) is a complex pathophysiological process involved with the activation of oxidative stress, inflammation and apoptosis. Sappanone A (SA), a homoisoflavanone isolated from the heartwood of Caesalpinia sappan L., could exhibit antioxidant, anti-inflammatory and anti-apoptotic activities. Therefore, we assumed that SA has a potential use for preventing against MIRI. The present study aimed to investigate the effect of SA treatment on MIRI and its mechanism. Cardiomyocytes (H9c2 cells) were treated with SA for 1 h, followed by 6 h of hypoxia/3 h of reoxygenation. Cell viability assay was detected by CCK-8 assay. Apoptosis was measured by flow cytometry and Hoechst staining. Mitochondrial permeability transition pore (mPTP) opening and mitochondrial transmembrane potential ( ψm) were measured by spectrophotometry and JC-1 staining. The changes of mitochondrial apoptosis-related proteins and PI3K-Akt-Gsk-3β signaling pathway were evaluated by Western blotting. The results showed that SA pretreatment enhanced the cell viability and decreased the activity of myocardial enzyme in a dose-dependent manner. Moreover, SA pretreatment significantly inhibited apoptosis, blocked mPTP opening, suppressed the release of ψm, prevented the cytochrome c releasing from mitochondria into cytoplasm, and repressed the cleavage of caspase-9 and caspase-3. Furthermore, SA pretreatment increased the phosphorylation levels of Akt and Gsk-3β but not of Stat-3. Meanwhile, the protective effect of SA was abrogated by PI3K inhibitor (LY294002). In conclusion, our results demonstrate that SA could prevent hypoxia/reoxygenation-induced cardiomyocytes injury through inhibition of mitochondrial apoptosis and activation of PI3K-Akt-Gsk-3β pathway. Thus, SA may have a potential use for the prevention of MIRI.

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Section: Discussionmentioning

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sappanone A alleviates hypoxia/reoxygenation-induced cardiomyocytes injury through inhibition of mitochondrial apoptosis and activation of PI3K–Akt–Gsk-3β pathway

Shi¹,

Gao²,

Ji³

et al. 2020

Bioscience Reports

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“…Inflammatory cells are able to infiltrate renal tissues after CDDP injection [15]. The inhibition of inflammatory cytokines could attenuate renal tissue damage by CDDP [26]. Our results showed that SDAPR markedly reduced the levels of TNF-α and IL- 6 in serum and kidneys.…”

Section: Discussionmentioning

confidence: 64%

The Protective Effects of Sika Deer Antler Protein on Cisplatin-Induced Nephrotoxicity

Yang

Wang³

et al. 2017

Cell Physiol Biochem

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Background/Aims: This study measured the effect of Sika deer (Cervus nippon Temminck) antler protein (SDAPR), glycoproteins (SDAG), and polysaccharides (SDAPO) on cisplatin-induced cytotoxicity in HEK 293 cells, and investigated the effect of SDAPR against cisplatin-induced nephrotoxicity in mice. Methods: Cell viability was measured by MTT assay. ICR mice were randomly divided into five groups: control, cisplatin with vehicle, and cisplatin with SDAPR at three concentrations: 5, 10, or 20 mg/kg, p.o., 10 d. Cisplatin was injected on 7^th day (25 mg/kg, i.p.). Renal function, oxidative stress, levels of inflammatory factors, and expression of apoptosis-related proteins were measured in vivo. Renal tissues were stained with TUNEL and H&E to observe renal cell apoptosis and pathological changes. Results: Pretreatment with SDAPR (125-2000 µg/mL) significantly improved cell viability, with an EC₅₀ of approximately 1000 µg/mL. SDAPR also ameliorated cisplatin-induced histopatholo- gic changes, and decreased blood urea nitrogen (BUN) and creatinine (Cr) (P < 0.05). Western blotting analysis showed SDAPR clearly decreased expression levels of cleaved-caspase-3 and Bax, and increased the expression level of Bcl-2 (P < 0.01). Additionally, SDAPR markedly regulated oxidative stress markers and inflammatory cytokines (P<0.05). TUNEL staining showed decreased apoptosis after SDAPR treatment (P < 0.01). Conclusions: These results indicate that SDAPR can be an effective dietary supplement, to relieve cisplatin-induced nephrotoxicity by improved antioxidase activity, suppressed inflammation, and inhibited apoptosis in vivo.

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“…The mice were challenged by intranasal inhalations with 100 μg OVA at days 21, 22, and 23; 20 mg SA was dissolved in 0.2 ml dimethyl sulfoxide and then dissolved in PBS to the concentrations of 12.5, 25 and 50 mg/kg. The doses of SA used in this study were based on previous studies [11,12]. SA (12.5, 25 and 50 mg/kg) was intraperitoneally given 1 h before the OVA challenge.…”

Section: Methodsmentioning

confidence: 99%

Sappanone A Attenuates Allergic Airway Inflammation in Ovalbumin-Induced Asthma

Liu

Yu²,

Wang³

2016

Int Arch Allergy Immunol

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Background: Sappanone A (SA) is isolated from the heartwood of Caesalpinia sappan and exerts a wide range of pharmacological activities. In the present study, we investigated the protective effects of SA on allergic asthma in a murine model of ovalbumin (OVA)-induced asthma. Methods: BALB/c mice were sensitized and challenged. Then, the mice were intraperitoneally injected with SA (12.5, 25 and 50 mg/kg) 1 h before OVA challenge; 24 h after the last challenge, the mice were sacrificed, and data were collected by different experimental methods. Results: The results showed that SA dose-dependently reduced inflammatory cell counts, levels of cytokines IL-4, IL-5 and IL-13, and OVA-specific IgE in bronchoalveolar lavage fluid. The level of IFN-γ decreased by OVA was upregulated by the treatment with SA. Furthermore, SA was found to attenuate the airway inflammation and mucus hypersecretion induced by the OVA challenge. In addition, SA dose-dependently upregulated the expression of Nrf2 and HO-1. SA inhibited OVA-induced asthma by activating the Nrf2 signaling pathway. Conclusions: These data suggest that SA may have a potential use as a therapeutic agent for asthma.

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Sappanone A protects mice against cisplatin-induced kidney injury

Cited by 29 publications

References 22 publications

Sappanone A alleviates hypoxia/reoxygenation-induced cardiomyocytes injury through inhibition of mitochondrial apoptosis and activation of PI3K–Akt–Gsk-3β pathway

Sappanone A alleviates hypoxia/reoxygenation-induced cardiomyocytes injury through inhibition of mitochondrial apoptosis and activation of PI3K–Akt–Gsk-3β pathway

The Protective Effects of Sika Deer Antler Protein on Cisplatin-Induced Nephrotoxicity

Sappanone A Attenuates Allergic Airway Inflammation in Ovalbumin-Induced Asthma

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