Sarcocystis spp. infection in South American deer huemul (Hippocamelus bisulcus) and pudu (Pudu puda) from Patagonian National Parks, Argentina

Reissig, Elizabeth Chang; Helman, Elisa; Moré, Gastón

doi:10.1007/s00436-020-06889-9

Cited by 3 publications

(8 citation statements)

References 26 publications

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“…Sarcocystis spp. identified in pudu and huemul were morphologically and molecularly similar from other species previously identified in cervids ( Chang Reissig et al, 2020 ). Until now, there is no specific description of the Sarcocystis spp.…”

Section: Introductionsupporting

confidence: 77%

“…using cervids as IH like S. cervicanis, S. grueneri , S. linearis, S . morae , S. taeniata, S. wapiti and the observed in sarcocysts from pudu from Argentina ( Chang Reissig et al, 2020 ). The bradyzoites contained amylopectin granules and were vacuolated, and no proper descriptions were achieved ( Fig.…”

Section: Resultsmentioning

confidence: 98%

“…At optical microscopy, no evident protrusions were observed, similar to features from other Sarcocystis spp. detected in cervids in Argentina ( Chang Reissig et al, 2016 , 2020 ). The cyst wall ultrastructure from four cysts obtained from two marsh deer from Iberá Wetlands showing ribbon-like protrusions resembled other species in cervids muscles like S. cervicanis S. grueneri, S. linearis, S. morae, S. taeniata and S. wapiti ( Gjerde, 2014a ; Dubey et al, 2016 ; Gjerde et al, 2017a , b ; Cerqueira-Cézar et al, 2018 ; Delgado de Las Cuevas et al, 2019 ; Rudaitytė-Lukošienė et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

“…All the muscles for each animal were processed as a pooled muscles sample following the methodology of direct microscopic examination previously described ( Moré et al, 2011 ; Chang Reissig et al, 2020 ). Briefly, 5–10 g of muscles were ground in a tissue homogenizer with the addition of 50 ml Phosphate Buffered Saline (PBS - pH 7.2), filtered and centrifuged.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, studies on the identification of Sarcocystis spp. in exotic red deer ( C. elaphus ) ( Chang Reissig et al, 2016 ) and the native deer species pudu ( Pudu puda ) and huemul ( Hipocamelus bisulcus ) have been performed in Argentina ( Chang Reissig et al, 2020 ). In the mentioned studies, S. taeniata was identified in C. elaphus, and three different species were recorded in the native deer species.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Identification of a new Sarcocystis sp. in marsh deer (Blastocerus dichotomus) from wetlands of Argentina

Berra¹,

Moré²,

Helman³

et al. 2023

International Journal for Parasitology: Parasites and Wildlife

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Section: Introductionsupporting

confidence: 77%

Section: Resultsmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Identification of a new Sarcocystis sp. in marsh deer (Blastocerus dichotomus) from wetlands of Argentina

Berra¹,

Moré²,

Helman³

et al. 2023

International Journal for Parasitology: Parasites and Wildlife

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Identification of Sarcocystis spp. in synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina

Bentancourt Rossoli,

Moré,

Soto-Cabrera

et al. 2023

Parasitol Res

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The occurrence of Sarcocystis species was investigated in synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina. Nine species were captured (n = 356). Sarcocysts were detected in muscles of 8.7% (31/356) and 3.7% (4/106) of the rodents by histopathology and direct microscopy, respectively. PCRs-sequencing targeting the 18S rRNA, cox1 and ITS1 regions were performed on samples with positive histopathology. Four different 18S rRNA sequences or sequence groups with high intra-group identities (99.6-100%) were detected in Mus musculus, Oxymycterus rufus, Akodon azarae and Necromys lasiurus. Eight sequences showed 99.5-99.7% identity with S. dispersa. Thirteen sequences showed low identity (95.3-96.4%) with other Sarcocystis spp. The obtained coxI sequences (n = 9) were almost identical among them and showed a high similarity with S. strixi (99.2-99.5%) and S. lutrae (99.1%), despite the 18S rRNA sequences from the same samples suggested the occurrence of at least two species. This suggests that coxI may not show high variability in Sarcocystis spp. that use rodents as intermediate hosts. Six ITS1 sequences were obtained, showing high identity but low coverage with several Sarcocystis spp. Multilocus sequence typing and BLAST analysis did not allow accurate species identi cation. Possible reasons are the detection of new species or the lack of molecular information from previously described ones. Phylogeny suggests that the detected Sarcocystis spp. may use raptor birds or snakes as de nitive hosts. This study represents the rst molecular identi cation of Sarcocystis spp. in naturally infected rodents of the Cricetidae and Muridae families in South America.

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Identification of Sarcocystis spp. in synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina

Rossoli,

Moré,

Soto-Cabrera

et al. 2023

Preprint

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The occurrence of Sarcocystis species was investigated in synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina. Nine species were captured (n = 356). Sarcocysts were detected in muscles of 8.7% (31/356) and 3.7% (4/106) of the rodents by histopathology and direct microscopy, respectively. PCRs-sequencing targeting the 18S rRNA, cox1 and ITS1 regions were performed on samples with positive histopathology. Four different 18S rRNA sequences or sequence groups with high intra-group identities (99.6–100%) were detected in Mus musculus, Oxymycterus rufus, Akodon azarae and Necromys lasiurus. Eight sequences showed 99.5–99.7% identity with S. dispersa. Thirteen sequences showed low identity (95.3–96.4%) with other Sarcocystis spp. The obtained coxI sequences (n = 9) were almost identical among them and showed a high similarity with S. strixi (99.2–99.5%) and S. lutrae (99.1%), despite the 18S rRNA sequences from the same samples suggested the occurrence of at least two species. This suggests that coxI may not show high variability in Sarcocystis spp. that use rodents as intermediate hosts. Six ITS1 sequences were obtained, showing high identity but low coverage with several Sarcocystis spp. Multilocus sequence typing and BLAST analysis did not allow accurate species identification. Possible reasons are the detection of new species or the lack of molecular information from previously described ones. Phylogeny suggests that the detected Sarcocystis spp. may use raptor birds or snakes as definitive hosts. This study represents the first molecular identification of Sarcocystis spp. in naturally infected rodents of the Cricetidae and Muridae families in South America.

show abstract

Sarcocystis spp. infection in South American deer huemul (Hippocamelus bisulcus) and pudu (Pudu puda) from Patagonian National Parks, Argentina

Cited by 3 publications

References 26 publications

Identification of a new Sarcocystis sp. in marsh deer (Blastocerus dichotomus) from wetlands of Argentina

Identification of a new Sarcocystis sp. in marsh deer (Blastocerus dichotomus) from wetlands of Argentina

Identification of Sarcocystis spp. in synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina

Identification of Sarcocystis spp. in synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina

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