Sargaquinoic acid attenuates inflammatory responses by regulating NF-κB and Nrf2 pathways in lipopolysaccharide-stimulated RAW 264.7 cells

Joung, Eun-Ji; Lee, Bonggi; Gwon, Wi-Gyeong; Shin, Tai-Sun; Jung, Bok-Mi; Yoon, Na-Young; Choi, Jong‐Yun; Oh, Chang Bo; Kim, Hyeung‐Rak

doi:10.1016/j.intimp.2015.09.007

Cited by 39 publications

(30 citation statements)

References 30 publications

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“…These results are consistent with the finding that FECG may block the LPS-induced phosphorylation of NF-κB p65 and decrease the degradation of IκBα . Moreover, the results of expression analysis of p65 in cells are different from the observations in cytoplasm and nucleus (Joung et al, 2015).…”

Section: Effect Of Fecg On the Activation And Nuclear Translocation Ocontrasting

confidence: 65%

Flavonoids derived from Exocarpium Citri Grandis inhibit LPS-induced inflammatory response via suppressing MAPK and NF-κB signalling pathways

Peng

Lü

et al. 2019

Food and Agricultural Immunology

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Pan (2019) Flavonoids derived from ExocarpiumCitriGrandis inhibit LPS-induced inflammatory response via suppressing MAPK and NF-κB signalling pathways, Food and Agricultural Immunology, 30:1, 564-580, ABSTRACT Exocarpium Citri Grandis (ECG) is used as an antitussive and antiinflammatory agent in traditional Chinese medicine. However, the mechanism of its anti-inflammatory effect remains unknown. In this study, flavonoids of ECG (FECG) were extracted to investigate the anti-inflammatory effect using a RAW264.7 macrophage model. The results suggested that FECG could remarkably inhibit the production of nitric oxide (NO). Meanwhile, FECG decreased the mRNA and protein expression of inflammatory cytokines including tumour necrosis factor alpha (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6), while showed an opposite effect on interleukin-10 (IL-10). Furthermore, FECG suppressed the phosphorylation of p38 (p38 kinase), JNK (c-Jun N-terminal kinase), ERK (extracellular signal-regulated kinase) and NF-κB. Taken together, our results indicated that FECG might regulate the activation of MAPK and NF-κB signalling pathways to exert anti-inflammatory effects. This study provides some insights into the mechanism underlying the anti-inflammatory activity of ECG, which may facilitate a better application of ECG. ARTICLE HISTORY

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Section: Effect Of Fecg On the Activation And Nuclear Translocation Ocontrasting

confidence: 65%

Flavonoids derived from Exocarpium Citri Grandis inhibit LPS-induced inflammatory response via suppressing MAPK and NF-κB signalling pathways

Peng

Lü

et al. 2019

Food and Agricultural Immunology

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“…To isolate RPMCs, rats were anesthetized with ether and injected with 10 mL of calcium-free N-(2-hydro-xyethyl) piperazine-N-2-ethanesulfonic acid (HEPES)-Tyrode buffer (137 mM NaCl, 5.6 mM glucose, 12 mM NaHCO 3 , 2.7 mM KCl, 0.3 mM NaH 2 PO 4 and 0.1% gelatin) into the peritoneal cavity, and the abdomen was gently massaged for approximately 90 s. The peritoneal cavity was opened carefully, and the fluid containing peritoneal cells was collected by a Pasteur pipette, and RPMCs were purified by using a Percoll (Pharmacia, Uppsala, Sweden) as described previously 52 . RPMC preparations were about 95% pure as assessed by toluidine blue staining and more than 98% of the cells were viable as judged by trypan blue uptake.…”

Section: Methodsmentioning

confidence: 99%

Polydatin inhibits mast cell-mediated allergic inflammation by targeting PI3K/Akt, MAPK, NF-κB and Nrf2/HO-1 pathways

Piao

Jiang

et al. 2017

Sci Rep

107

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Polydatin(PD) shows anti-allergic inflammatory effect, and this study investigated its underlying mechanisms in in vitro and in vivo models. IgE-mediated passive cutaneous anaphylaxis (PCA) and passive systemic anaphylaxis (PSA) models were used to confirm PD effect in vivo. Various signaling pathway proteins in mast cell were examined. RT-PCR, ELISA and western blotting were applied when appropriate. Activity of Lyn and Fyn kinases in vitro was measured using the Kinase Enzyme System. PD dose-dependently reduced the pigmentation of Evans blue in the PCA model and decreased the concentration of serum histamine in PSA model, and attenuated the degranulation of mast cells without generating cytotoxicity. PD decreased pro-inflammatory cytokine expression (TNF-α, IL-4, IL-1β, and IL-8). PD directly inhibited activity of Lyn and Syk kinases and down-regulated downstream signaling pathway including MAPK, PI3K/AKT and NF-kB. In addition, PD also targets Nrf2/HO-1 pathway to inhibit mast cell-derived allergic inflammatory reactions. In conclusion, the study demonstrates that PD is a possible therapeutic candidate for allergic inflammatory diseases. It directly inhibited activity of Lyn and Syk kinases and down-regulates the signaling pathway of MAPK, PI3K/AKT and NF-κB, and up-regulates the signaling pathway of Nrf2/HO-1 to inhibit the degranulation of mast cells.

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“…Recently, we reported that ethanolic extract of S. serratifolium showed antioxidant and antiinflammatory activities and active compounds were identified as sargahydroquinoic acid (SHQA), sargachromenol (SCM), and sargaquinoic acid (SQA) Lim et al 2018;Oh et al 2016). Moreover, SCM and SQA isolated from the ethanolic extract of S. serratifolium showed a potent anti-inflammatory activity in LPS-stimulated macrophages and antiatherogenic activities in TNF-α-treated human umbilical vein endothelial cells (HUVECs), respectively Gwon et al 2015;Joung et al 2015). We prepared a meroterpenoid-rich fraction from the ethanolic extract of S. serratifolium (MES) by removing salts and water-soluble saccharides, and the combined amount of SHQA, SCM, and SQA was estimated to be 46 g in 100 g of MES (Gwon et al 2018;Kwon et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Hexane fraction from the ethanolic extract of Sargassum serratifolium suppresses cell adhesion molecules via regulation of NF-κB and Nrf2 pathway in human umbilical vein endothelial cells

et al. 2019

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Sargassum serratifolium ethanolic extract has been known for strong antioxidant and anti-inflammatory properties. We prepared hexane fraction from the ethanolic extract of S. serratifolium (HSS) to improve biological activities. In this study, we investigated the effects of HSS on the inhibition of tumor necrosis factor (TNF)-α-induced monocyte adhesion to human umbilical vein endothelial cells (HUVECs). We found that HSS suppressed the production of cell adhesion molecules such as intracellular adhesion molecule-1 and vascular cell adhesion molecule-1 in TNF-α-induced HUVECs. Moreover, TNF-α-induced production of monocyte chemoattractant protein 1 and keratinocyte chemoattractant was inhibited by HSS treatment. HSS suppressed TNF-α-induced nuclear factor kappa B (NF-κB) activation via preventing proteolytic degradation of inhibitor κB-α. HSS induced the production of heme oxygenase 1 via translocation of Nrf2 into the nucleus in TNF-α-treated HUVECs. Overall, HSS alleviated vascular inflammation through the downregulation of NF-κB activation and the upregulation of Nrf2 activation in TNF-α-induced HUVECs. These results indicate that HSS may be used as therapeutic agents for vascular inflammatory disorders.

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Sargaquinoic acid attenuates inflammatory responses by regulating NF-κB and Nrf2 pathways in lipopolysaccharide-stimulated RAW 264.7 cells

Cited by 39 publications

References 30 publications

Flavonoids derived from Exocarpium Citri Grandis inhibit LPS-induced inflammatory response via suppressing MAPK and NF-κB signalling pathways

Flavonoids derived from Exocarpium Citri Grandis inhibit LPS-induced inflammatory response via suppressing MAPK and NF-κB signalling pathways

Polydatin inhibits mast cell-mediated allergic inflammation by targeting PI3K/Akt, MAPK, NF-κB and Nrf2/HO-1 pathways

Hexane fraction from the ethanolic extract of Sargassum serratifolium suppresses cell adhesion molecules via regulation of NF-κB and Nrf2 pathway in human umbilical vein endothelial cells

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