Sargassum horneri (Turner) C. Agardh ethanol extract attenuates fine dust-induced inflammatory responses and impaired skin barrier functions in HaCaT keratinocytes

Dias, Mawalle Kankanamge Hasitha Madhawa; Madusanka, Dissanayaka Mudiyanselage Dinesh; Han, Eui Jeong; Kim, Hyunsoo; Jeon, You‐Jin; Jee, Youngheun; Kim, Kil-Nam; Lee, Kyounghoon; Fernando, Ilekuttige Priyan Shanura; Ahn, Ginnae

doi:10.1016/j.jep.2021.114003

Cited by 37 publications

(40 citation statements)

References 34 publications

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“…These results demonstrated systematic damage by PM2.5 on skin cells at the molecular level, supported by later studies demonstrating that skin barrier function is affected by PMs; one such study reported the clinical significance of these findings [12,13]. Over the last year, similar studies reached similar conclusions, confirming the crucial impact of PMs on keratinocytes [14,15]. Moreover, ER stress elicited by PM could also raise [Ca 2+ ] i , eventually increasing melanin production [16,17].…”

Section: Introductionsupporting

confidence: 63%

The Protective Effect of Hamamelis virginiana Stem and Leaf Extract on Fine Dust-Induced Damage on Human Keratinocytes

Ji-Young¹,

Yang

Moon³

et al. 2021

Cosmetics

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Witch hazel extracts have been used for decades as cosmetic ingredients in skin care products. Our present study aims to evaluate its potential in anti-pollution products using a previously reported in vitro model. Calcium is a universal second messenger, and we used human respiratory and skin cells to detect changes in intracellular Ca2+ concentrations upon particulate matter contact. Both an increase in pro-inflammatory markers and a decrease in tight junction proteins were confirmed, as previously reported. Witch hazel stem and leaf extract showed significant attenuation of Ca2+ response upon the challenge; it displayed systematic regulations of the signal generator, PAR-2; a pro-inflammatory marker, NF-κB; and a tight junction protein, Occludin. We identified hexagalloylglucose from the extract and concluded that it is a major component regulating protection from particulate matter. Based on these results, witch hazel extract containing hexagalloylglucose is an active ingredient in anti-pollution skin care products.

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Section: Introductionsupporting

confidence: 63%

The Protective Effect of Hamamelis virginiana Stem and Leaf Extract on Fine Dust-Induced Damage on Human Keratinocytes

Ji-Young¹,

Yang

Moon³

et al. 2021

Cosmetics

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“…In this study, TLC analysis (Supplementary Material Figure S2) verified the existence of antioxidative chemicals in MOH seen from the change of color in KMnO4-stained TLC. The discoloration of KMnO4 is caused by a reduction in the oxidative state of Mn from +7 to +2 [41]. Furthermore, the occurrence of nucleophiles, aldehydes, and ketones was confirmed by p-anisaldehyde and Vanillin stains, which commonly undergo Aldol condensation and acetalization [42].…”

Section: Discussionmentioning

confidence: 99%

Moringa oleifera Hot Water Extract Protects Vero Cells from Hydrogen Peroxide-Induced Oxidative Stress by Regulating Mitochondria-Mediated Apoptotic Pathway and Nrf2/HO-1 Signaling

Kirindage

Fernando

Jayasinghe

et al. 2022

Foods

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The present study discloses the identification of phenolic compounds in Moringa oleifera hot water extract (MOH) and the evaluation of its antioxidant activity on H2O2-induced oxidative stress in Vero cells. Upon analysis, MOH was found to contain phenolic compounds and indicated 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS+) radical scavenging with IC50 values of 102.52 and 122.55 µg/mL, respectively. The ferric reducing antioxidant power (FRAP) of MOH indicated a dose-dependent increase with a maximum absorbance at 125 μg/mL and the oxygen radical absorbance capacity (ORAC) of MOH was 1004.95 µmol TE/mg. Results showed that MOH dose-dependently reduced intracellular ROS generation in H2O2-stimulated Vero cells while increasing the cell viability. Fluorescence microscopy and flowcytometric analyses have supported the above findings. MOH markedly suppressed the H2O2-induced mitochondrial depolarization and apoptosis through suppression of the mitochondrial-mediated apoptosis pathway and activated the Nrf2/HO-1 signaling pathway by possibly involving H2O2 generation in cell media. Findings of western blot were supported by immunocytochemistry of Nrf2 nuclear translocation. Thus, MOH bioactivity would potentiate its applications in manufacturing functional food.

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“…SH is an extremely stable form of HA, which is often used as a protective agent for the bladder mucosa in cystitis. Therefore, SH was used as [42]. When existing antioxidant defense mechanisms fail to regulate excessive ROS production, cells enter a state of oxidative stress that disrupts the redox balance.…”

Section: Discussionmentioning

confidence: 99%

“…When existing antioxidant defense mechanisms fail to regulate excessive ROS production, cells enter a state of oxidative stress that disrupts the redox balance. This activates the NF-κB and MAPK signaling pathways, leading to the production of inflammatory cytokines [42,43]. Inflammation stimulates further production of ROS, creating a continuous adverse cycle.…”

Section: Discussionmentioning

confidence: 99%

The protective effect of manganese superoxide dismutase from thermophilic bacterium HB27 on hydrochloric acid-induced chemical cystitis in rats

Chen

Gao

et al. 2021

Int Urol Nephrol

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Purpose To evaluate the effects of manganese superoxide dismutase (Mn-SOD) from thermophilic bacterium HB27 (name as Tt-SOD) on chemical cystitis. Methods Control and experimental rats were infused by intravesical saline or hydrochloric acid (HCl) on the first day of the experiments. Saline, sodium hyaluronate (SH) or Tt-SOD were infused intravesically once a day for three consequent days. On the fifth day, the rats were weighted and sacrificed following a pain threshold test. The bladder was harvested for histological and biochemical analyses. Results Tt-SOD could reduce the bladder index, infiltration of inflammatory cells in tissues, serum inflammatory factors and SOD levels, mRNA expression of inflammatory factors in tissues, and increase perineal mechanical pain threshold and serum MDA and ROS levels in HCl-induced chemical cystitis. Furthermore, Tt-SOD alleviated inflammation and oxidative stress by the negative regulation of the NF-κB p65 and p38 MAPK signaling pathway. Conclusions Intravesical instillation of Tt-SOD provides protective effects against HCl-induced cystitis.

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Sargassum horneri (Turner) C. Agardh ethanol extract attenuates fine dust-induced inflammatory responses and impaired skin barrier functions in HaCaT keratinocytes

Cited by 37 publications

References 34 publications

The Protective Effect of Hamamelis virginiana Stem and Leaf Extract on Fine Dust-Induced Damage on Human Keratinocytes

The Protective Effect of Hamamelis virginiana Stem and Leaf Extract on Fine Dust-Induced Damage on Human Keratinocytes

Moringa oleifera Hot Water Extract Protects Vero Cells from Hydrogen Peroxide-Induced Oxidative Stress by Regulating Mitochondria-Mediated Apoptotic Pathway and Nrf2/HO-1 Signaling

The protective effect of manganese superoxide dismutase from thermophilic bacterium HB27 on hydrochloric acid-induced chemical cystitis in rats

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