Saturated hydrogen saline attenuates endotoxin-induced lung dysfunction

Zhang, Yan; Liu, Yiming; Zhang, Jin

doi:10.1016/j.jss.2015.04.055

Cited by 31 publications

(47 citation statements)

References 39 publications

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“…This is followed by elongation and closure of the phagopore, forming a complete autophagosome. Subsequent fusion of lysosomes with autophagosomes forms autolysosomes, in which substrates of autophagy are exposed to the hydrolytic interior of the lysosome, ultimately resulting in substrate degradation (9). …”

Section: Autophagymentioning

confidence: 99%

“…Under normal physiological conditions, low levels of autophagy occur within cells (9). However, in the absence of nutrients in the environment (resulting in starvation), the rate of autophagy increases, with greater degradation of macromolecules providing the minimum energy required to maintain cell survival.…”

Section: Autophagymentioning

confidence: 99%

“…However, in the absence of nutrients in the environment (resulting in starvation), the rate of autophagy increases, with greater degradation of macromolecules providing the minimum energy required to maintain cell survival. Certain toxins and pathogens are also degraded by autophagy, along with degenerative cytoplasmic components (4,9). Thus, autophagy serves a key preventative role during the early stages of many infectious diseases; however its overactivation may result in excessive degradation of intracellular substances and cell death (15).…”

Section: Autophagymentioning

confidence: 99%

“…Pretreatment with saturated hydrogen saline may have a protective effect against LPS-induced ALI (9); however, it remains unknown whether saturated hydrogen saline has therapeutic effects on LPS-induced autophagy in lung tissue. Signaling pathways involving nuclear factor (NF)-κB, Beclin-1, mechanistic target of rapamycin (mTOR), heme oxygenase-1 (HO-1) and p38 mitogen-activated protein kinase (MAPK) have been implicated in the process of autophagy, and thus may be involved in the protective effects of saturated hydrogen saline on LPS-induced ALI.…”

Section: Introductionmentioning

confidence: 99%

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Saturated hydrogen saline ameliorates lipopolysaccharide-induced acute lung injury by reducing excessive autophagy (Review)

Liu

Zhang

2017

Exp Ther Med

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The pathogenesis of acute lung injury (ALI) induced by lipopolysaccharide (LPS) involves excessive pulmonary inflammation and oxidative stress. In turn, autophagy is associated with inflammatory diseases and organ dysfunction, and studies have demonstrated that LPS treatment may trigger autophagy. Thus, excessive autophagy may stimulate the strong inflammatory response observed in the development of LPS-induced ALI. Saturated hydrogen saline may alleviate LPS-induced ALI by inhibiting autophagy, however its underlying mechanisms of action remain unknown. It has been suggested that saturated hydrogen saline may downregulate expression of nuclear factor (NF)-κB, leading to a decrease in Beclin-1 transcription and inhibition of autophagy. Inhibition of autophagy also occurs via the phosphorylation of Unc-51-like autophagy activating kinase 1 and autophagy-related protein-13 by mechanistic target of rapamycin, which in turn may be upregulated by saturated hydrogen saline. In addition, signaling pathways involving heme oxygenase-1 and p38 mitogen-activated protein kinase are associated with the alleviative effects of saturated hydrogen saline on LPS-induced autophagy. The present review focuses on potential molecular mechanisms regarding the effects of saturated hydrogen saline in the reduction of autophagy during LPS-induced ALI.

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Section: Autophagymentioning

confidence: 99%

Section: Autophagymentioning

confidence: 99%

Section: Autophagymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Saturated hydrogen saline ameliorates lipopolysaccharide-induced acute lung injury by reducing excessive autophagy (Review)

Liu

Zhang

2017

Exp Ther Med

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“…Thus, it is imperative to develop effective strategies for the treatment of ALI without significant or with only mild side effects. Some recent studies [8–10] found that hydrogen-rich saline could exert a potential effect against inflammation and oxidative stress-associated pathologies in ALI, which provides a novel strategy for the treatment of ALI. However, because methanogens in the intestine can partially produce hydrogen and convert hydrogen to methane in vivo [11, 12], there are controversies over the therapeutic effect of hydrogen and methane and the effect of methane on ALI is unclear in vivo.…”

Section: Introductionmentioning

confidence: 99%

Protective Effects of Methane‐Rich Saline on Rats with Lipopolysaccharide‐Induced Acute Lung Injury

Sun

Wang

et al. 2017

Oxidative Medicine and Cellular Longevity

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Objective. The aim of this research is to evaluate the protective effects of methane-rich saline (MS) on lipopolysaccharide- (LPS-) induced acute lung injury (ALI) and investigate its potential antioxidative, anti-inflammatory, and antiapoptotic activities. Methods. LPS-induced (20 mg/kg) ALI rats were injected with MS (2 ml/kg and 20 ml/kg) before the initiation of LPS induction. Survival rate was determined until 96 h after LPS was induced. Lung injury was assayed by oxygenation index, lung permeability index (LPI), wet-to-dry weight (W/D), and histology. The cells in the bronchoalveolar lavage fluid (BALF) were counted. Oxidative stress was examined by the level of malondialdehyde (MDA) and superoxide dismutase (SOD). Inflammatory factors including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in BALF were determined by ELISA. Lung tissue apoptosis was detected by TUNEL staining and western blotting of caspase-3. Results. It was found that methane significantly prolonged the rat survival, decreased the lung W/D ratio and the content of the inflammatory factors, and reduced the amount of caspase-3 and apoptotic index. In addition, MS increased the level of SOD and decreased the level of MDA significantly. Conclusions. MS protects the LPS-challenged ALI via antioxidative, anti-inflammatory, and antiapoptotic effect, which may prove to be a novel therapy for the clinical management of ALI.

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Hydrogen-rich water ameliorates bronchopulmonary dysplasia (BPD) in newborn rats

et al. 2016

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Bronchopulmonary dysplasia (BPD) is characterized by developmental arrest of the alveolar tissue. Oxidative stress is causally associated with development of BPD. The effects of hydrogen have been reported in a wide range of disease models and human diseases especially caused by oxidative stress. We made a rat model of BPD by injecting lipopolysaccharide (LPS) into the amniotic fluid at E16.5. The mother started drinking hydrogen-rich water from E9.5 and also while feeding milk. Hydrogen normalized LPS-induced abnormal enlargement of alveoli at P7 and P14. LPS increased staining for nitrotyrosine and 8-OHdG of the lungs, and hydrogen attenuated the staining. At P1, LPS treatment decreased expressions of genes for FGFR4, VEGFR2, and HO-1 in the lungs, and hydrogen increased expressions of these genes. In contrast, LPS treatment and hydrogen treatment had no essential effect on the expression of SOD1. Inflammatory marker proteins of TNFα and IL-6 were increased by LPS treatment, and hydrogen suppressed them. Treatment of A549 human lung adenocarcinoma epithelial cells with 10% hydrogen gas for 24 hr decreased production of reactive oxygen species in both LPS-treated and untreated cells. Lack of any known adverse effects of hydrogen makes hydrogen a promising therapeutic modality for BPD. Pediatr Pulmonol. 2016; 51:928-935. © 2016 Wiley Periodicals, Inc.

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Saturated hydrogen saline attenuates endotoxin-induced lung dysfunction

Cited by 31 publications

References 39 publications

Saturated hydrogen saline ameliorates lipopolysaccharide-induced acute lung injury by reducing excessive autophagy (Review)

Saturated hydrogen saline ameliorates lipopolysaccharide-induced acute lung injury by reducing excessive autophagy (Review)

Protective Effects of Methane‐Rich Saline on Rats with Lipopolysaccharide‐Induced Acute Lung Injury

Hydrogen-rich water ameliorates bronchopulmonary dysplasia (BPD) in newborn rats

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