SAXS Analysis and Characterization of Anticancer Activity of PNP-UDP Family Protein from Putranjiva roxburghii

Verma, Preeti; Varshney, Ritu; Yadav, Shiv Pratap Singh; Kar, Bibekananda; Roy, Partha; Sharma, Ashwani

doi:10.1007/s10930-022-10060-x

Cited by 1 publication

(4 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Also, some previous work has claimed the participation of purine metabolism enzymes in CK metabolism (Schoor et al ., 2011; Zhang et al ., 2013). Since PRpnp is a salvage pathway enzyme of purine biosynthesis (Verma et al ., 2017, 2022). Thus, we anticipated the role of PRpnp in catalysing CK interconversion reactions.…”

Section: Resultsmentioning

confidence: 99%

“…Similarly, one ‘yet to be studied’ group of proteins is related to WI protein and VSPs and shows homology to nucleoside phosphorylases. In our previous studies, we characterized one of the proteins from this group, that is, PRpnp and proved its neofunctionalization to a TI (Chaudhary et al ., 2008; Verma et al ., 2017, 2022). We speculated PRpnp as a potential candidate that can promote multiple stress tolerance in plants.…”

Section: Discussionmentioning

confidence: 99%

“…PRpnp showed an insertion of 46-amino acid residues in its PNP-UDP domain, which is responsible for its trypsin inhibitory nature and, thus, it possesses dual activity, that is, PNP and protease (trypsin) inhibitory activity. The insertion of a trypsin inhibitory loop is the case of neofunctionalization of PRpnp to a potent trypsin inhibitor (TI) to cater to the needs of plant defence (Verma et al, 2017(Verma et al, , 2022.…”

Section: Introductionmentioning

confidence: 99%

“…To accomplish this, numerous structural and functional changes in plant proteins have been observed during the course of evolution (Bartlett and Whipple, 2013). Similarly, PRpnp and other homologous proteins evolved and attained a disruption in the PNP-UDP domain (Verma et al, 2017(Verma et al, , 2022. The purine salvaging capacity and neofunctionalization of PRpnp to TI, along with its evolutionary linkage to other plant defence proteins, prompted us to explore its defensive role against multiple environmental stresses.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

PRpnp, a novel dual activity PNP family protein improves plant vigour and confers multiple stress tolerance in Citrus aurantifolia

Singh

Chaudhary

Bharsakale

et al. 2023

Plant Biotechnology Journal

Self Cite

View full text Add to dashboard Cite

Summary Under field conditions, plants are often simultaneously exposed to several abiotic and biotic stresses resulting in significant reductions in growth and yield; thus, developing a multi‐stress tolerant variety is imperative. Previously, we reported the neofunctionalization of a novel PNP family protein, Putranjiva roxburghii purine nucleoside phosphorylase (PRpnp) to trypsin inhibitor to cater to the needs of plant defence. However, to date, no study has revealed the potential role and mechanism of either member of this protein group in plant defence. Here, we overexpressed PRpnp in Citrus aurantifolia which showed nuclear‐cytoplasmic localization, where it functions in maintaining the intracellular purine reservoir. Overexpression of PRpnp significantly enhanced tolerance to salt, oxidative stress, alkaline pH, drought and two pests, Papilio demoleus and Scirtothrips citri in transgenic plants. Global gene expression studies revealed that PRpnp overexpression up‐regulated differentially expressed genes (DEGs) related to ABA‐ and JA‐biosynthesis and signalling, plant defence, growth and development. LC–MS/MS analysis validated higher endogenous ABA and JA accumulation in transgenic plants. Taken together, our results suggest that PRpnp functions by enhancing the endogenous ABA and JA, which interact synergistically and it also inhibits trypsin proteases in the insect gut. Also, like other purine salvage genes, PRpnp also regulates CK metabolism and increases the levels of CK‐free bases in transgenic Mexican lime. We also suggest that PRpnp can be used as a potential candidate to develop new varieties with improved plant vigour and enhanced multiple stress resistance.

show abstract