Scaffolds for bone-tissue engineering

Lee, Seunghun S.; Du, Xiaoyu; Kim, Inseon; Ferguson, Stephen J.

doi:10.1016/j.matt.2022.06.003

Cited by 92 publications

(45 citation statements)

References 274 publications

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“…Evidently shown by the cross-sectional SEM images of the PLGA PMs following compound dehydration (Figure C), the exterior pores are shown as 21.8 ± 6.1 μm in diameter while the interconnected interior pores show average pore diameter of 26.6 ± 11.3 μm (Figure E), offering a three-dimensional (3D) microenvironment both suitable for cell infiltration (exterior) and adhesion (interior). In addition to the spatial environment provided by the PLGA PMs, biophysical cues have been lately suggested as another factor that may also impact cell behaviors . Indeed, it has been reported that substrate stiffness, one of the key biophysical factors, participates in mediating cell attachment, migration, spreading, phenotype maintenance, and more.…”

Section: Resultsmentioning

confidence: 99%

Construction of PLGA Porous Microsphere-Based Artificial Pancreatic Islets Assisted by the Cell Centrifugation Perfusion Technique

et al. 2023

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Pancreatic islet transplantation is a promising treatment that could potentially reverse diabetes, but its clinical applicability is severely limited by a shortage of organ donors. Various cell loading approaches using polymeric porous microspheres (PMs) have been developed for tissue regeneration; however, PM-based multicellular artificial pancreatic islets' construction has been scarcely reported. In this study, MIN6 (a mouse insulinoma cell line) and MS1 (a mouse pancreatic islet endothelial cell line) cells were seeded into poly(lacticco-glycolic acid) (PLGA) PMs via an upgraded centrifugation-based cell perfusion seeding technique invented and patented by our group. Cell morphology, distribution, viability, migration, and proliferation were all evaluated. Results from glucose-stimulated insulin secretion (GSIS) assay and RNA-seq analysis suggested that MIN6 and MS1-loaded PLGA PMs exhibited better glucose responsiveness, which is partly attributable to vascular formation during PM-dependent islet construction. The present study suggests that the PLGA PM-based artificial pancreatic islets may provide an alternative strategy for the potential treatment of diabetes in the future.

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Section: Resultsmentioning

confidence: 99%

Construction of PLGA Porous Microsphere-Based Artificial Pancreatic Islets Assisted by the Cell Centrifugation Perfusion Technique

et al. 2023

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“…Bone loss and fracture caused by disease or injury re considered major health problems [ 1 ]. Today, the most common method to address bone defects is bone grafting, but unfortunately, it is still problematic for clinicians to choose between autografts, allografts or engineered tissues [ 2 ]. Recently, bone tissue engineering (BTE) has gained more attention as a potential treatment for bone defects.…”

Section: Introductionmentioning

confidence: 99%

“…The strategy for bone tissue engineering involves the following steps: (1) identification of a suitable cell source, isolation of cells and expansion of cells to sufficient amounts; (2) obtaining of a biocompatible material that can be used as a cell substrate and processed into the required shape (scaffold); (3) seeding of the scaffold with cells, which can then be cultivated in bioreactors; (4) placement of the material-cell construct into the target site in vivo [ 2 ]. In this regard, scaffolds are the key component of BTE, as they regulate bone healing and mimic the extracellular matrix (ECM) function in the bone tissue [ 2 , 3 ]. An ideal scaffold for bone tissue repair should have a three-dimensional porous structure with a highly interconnected pore network, being biocompatible and controllably bioresorbable [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…An ideal scaffold for bone tissue repair should have a three-dimensional porous structure with a highly interconnected pore network, being biocompatible and controllably bioresorbable [ 3 , 4 ]. The ideal strategy for regenerative bone therapies is to use a tunable scaffolding material that can modulate the process of healing while providing mechanical support [ 2 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…However, 3D organization of particles into scaffolds via direct ink writing (DIW) and stereolithography (SLA) is still an unexplored area [ 24 ]. Wide application of such 3D printing techniques in clinics with utilization of particles as versatile material for scaffold formation could help to solve the problems of auto- and allograft applications, which comprise a shortage of bone, donor site morbidity and additional operations on the patients [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

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Poly(lactic acid) and Nanocrystalline Cellulose Methacrylated Particles for Preparation of Cryogelated and 3D-Printed Scaffolds for Tissue Engineering

et al. 2023

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Different parts of bones possess different properties, such as the capacity for remodeling cell content, porosity, and protein composition. For various traumatic or surgical tissue defects, the application of tissue-engineered constructs seems to be a promising strategy. Despite significant research efforts, such constructs are still rarely available in the clinic. One of the reasons is the lack of resorbable materials, whose properties can be adjusted according to the intended tissue or tissue contacts. Here, we present our first results on the development of a toolbox, by which the scaffolds with easily tunable mechanical and biological properties could be prepared. Biodegradable poly(lactic acid) and nanocrystalline cellulose methacrylated particles were obtained, characterized, and used for preparation of three-dimensional scaffolds via cryogelation and 3D printing approaches. The composition of particles-based ink for 3D printing was optimized in order to allow formation of stable materials. Both the modified-particle cytotoxicity and the matrix-supported cell adhesion were evaluated and visualized in order to confirm the perspectives of materials application.

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Biomimetic Mineralized Organic–Inorganic Hybrid Scaffolds From Microfluidic 3D Printing for Bone Repair

Yang,

Li,

Ding

et al. 2024

Adv Funct Materials

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Bone defect is a common clinical orthopedic disease. The trend in this field is to develop tissue engineering scaffolds with appropriately designed components and structures for bone repair. Herein, inspired by the organic and inorganic components of bone matrix and the natural biomineralization mechanism, a MgSiO3@Fe3O4 nanoparticle composite polycaprolactone (PCL) hybrid mineralized scaffold for bone repair is developed by microfluidic 3D printing. The incorporation of MgSiO3@Fe3O4 within the PCL scaffold can effectively improve the bioactivity. In addition, a biomimetic mineralized layer is prepared on the surface of the scaffold, which endowed it with unique microstructural characteristics, enhanced cell adhesion and osteogenic activity, and thus improved the bone repair performance. Owing to these advantages, both in vivo and in vitro experiments have demonstrated that the designed scaffold has outstanding biocompatibility and bone repair performance. These features indicate that the organic–inorganic biomineralized hybrid scaffold can be a potential bone graft substitute for clinical bone repair.

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Scaffolds for bone-tissue engineering

Cited by 92 publications

References 274 publications

Construction of PLGA Porous Microsphere-Based Artificial Pancreatic Islets Assisted by the Cell Centrifugation Perfusion Technique

Construction of PLGA Porous Microsphere-Based Artificial Pancreatic Islets Assisted by the Cell Centrifugation Perfusion Technique

Poly(lactic acid) and Nanocrystalline Cellulose Methacrylated Particles for Preparation of Cryogelated and 3D-Printed Scaffolds for Tissue Engineering

Biomimetic Mineralized Organic–Inorganic Hybrid Scaffolds From Microfluidic 3D Printing for Bone Repair

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