Scale-Up and Long-Term Study of Electrodialysis with Ultrafiltration Membrane for the Separation of a Herring Milt Hydrolysate

Abstract

Electrodialysis with ultrafiltration membrane (EDUF) was selected to separate a herring milt hydrolysate (HMH) in a scale-up and long-term study for the recovery of bioactive peptides. The scale-up was performed to maximise peptide recovery by placing a total membrane area of 0.08 m2 for each anionic and cationic compartment. Twelve consecutive runs were carried out, for a total of 69 h, with minimal salt solution cleaning in between experiments. The final peptide migration rate showed that cationic peptides had a higher average migration rate (5.2 ± 0.8 g/m2·h), compared to anionic peptides (4.7 ± 1.1 g/m2·h). Migration was also selective according to peptide identifications and molecular mass distribution where only small molecular weights were found (<1000 Da) in both recovery compartments. The areal system resistance slightly decreased during each run and the averaged values were stable in between experiments since they were all found in the 95% confidence interval. In addition, total relative energy consumption was quite consistent with an average value of 39.95 ± 6.47 Wh/g all along the 12 consecutive runs. Finally, according to membrane characterization, there was no visual fouling on the different membranes present in the EDUF cell after 69 h of treatment. This may be due to the salt cleaning in between experiments which allowed removal of peptides from the membranes, thus allowing recovering initial system working parameters at the beginning of each run. The entire process was revealed to be very consistent and repeatable in terms of peptide migration, global system resistance, and energy consumption. To the best of our knowledge, this is the first time such EDUF conditions (membrane surface, duration, and minimal salt cleaning between experiments) are being tested on a complex hydrolysate.

Effects of Herring Milt Hydrolysates and Fractions in a Diet-Induced Obesity Model

“…The HMH1 supplementation contained significantly more peptides than the other two supplements with a concentration of 93.79% [ 18 ]. However, the HMH1 peptides had a lower molecular weight than those of the HMH2 and HMH5 supplements (62% of HMH1 peptides had a molecular weight higher than 10,000 Da, while all HMH1 peptides were smaller than 1000 Da), since the HMH1 product was the permeate and the HMH2 product was the retentate [ 16 , 19 ]. Also, the previous studies from Durand et al [ 16 , 17 , 18 ] showed that these three supplements had the same free amino acids, but in different concentrations.…”

“… EDUF configuration used for the separation and production of HMH4 and HMH5; P +/− neutral peptides, P + cationic peptides, P − anionic peptides [ 19 ]. …”

“…-Figure1. EDUF configuration used for the separation and production of HMH4 and HMH5; P +/− neutral peptides, P + cationic peptides, P − anionic peptides[19].Table1. Chemical composition of the different herring milt hydrolysates (HMH).…”

Research progress on extraction technology and biomedical function of natural sugar substitutes

“…As a new method, this technology confers various advantages during extraction of NSS, energy saving, high single-stage separation efficiency, environmental protection, no phase change, and a simple filtration process, among others (55). At present, a variety of membrane separation and extraction processes, such as ultrafiltration (UF), nanofiltration, electrodialysis, pervaporation and gas separation have been developed (56)(57)(58)(59)(60). Firstly, processes for separation and purification of alkaloids are greatly constrained by various challenges, including high pollution, high energy consumption and low continuity.…”