Scaling-up and ionic liquid-based extraction of pectinases from Aspergillus flavipes cultures

Wolf-Márquez, Vicente E.; Martínez-Trujillo, Aurora; Osorio, Guillermo Aguilar; Patiño, Faustino; Álvarez, María S.; Rodríguez, Ana; Sanromán, M. Ángeles; Deive, Francisco J.

doi:10.1016/j.biortech.2016.11.067

Cited by 34 publications

(5 citation statements)

References 35 publications

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“…The greater ability of the selected choline dihydrogen phosphate-based DES to interact with the enzyme may be the result of a complex balance of hydrophobicity, polarity and hydrogen bonding interaction of the enzyme and the ABS components. These data are in line with other partition studies carried out with both lipases and other enzymes like pectinases in the presence of choline chloride and non-ionic surfactants or polymers [49,50]. In summary, the present research work demonstrates the suitability of choline dihydrogen phosphate-based DES for the extraction of lipases from aqueous solutions where they are usually produced leading to a biocatalytic milieu that can be straightforward employed in applications such as biodiesel production of the resolution of racemic mixtures in pharma industries.…”

Section: Tie-lines Modelling and Application To Enzyme Separationsupporting

confidence: 91%

Choline dihydrogen phosphate-based deep eutectic solvent: A suitable bioplatform for lipase extraction

Álvarez

Rivas

Longo

et al. 2021

Separation and Purification Technology

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Section: Tie-lines Modelling and Application To Enzyme Separationsupporting

confidence: 91%

Choline dihydrogen phosphate-based deep eutectic solvent: A suitable bioplatform for lipase extraction

Álvarez

Rivas

Longo

et al. 2021

Separation and Purification Technology

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“…Since the residual material left after maceration extraction still possessed an intense color, it was decided to carry out an EAE. For that purpose, an enzymatic cocktail obtained from the fermentation of corn cob by Aspergillus flavipes FP‐500 was used and combined with two commercial enzyme cocktails namely Celluclast and Depol 740 L. The enzymatic cocktail from Aspergillus flavipes was used since this strain is known to produce pectinases and xylanases when growing on different agroindustrial residues, including the corn husk and corn cob (Torres‐Barajas & Aguilar‐Osorio, )(Martínez‐Trujillo, Aranda‐Barradas, & Aguilar‐Osorio, )(Gómez‐Sánchez, Martínez‐Trujillo, & Aguilar‐Osorio, )(Wolf‐Márquez et al., ).…”

Section: Methodsmentioning

confidence: 99%

Extraction and Identification of Anthocyanins in Corn Cob and Corn Husk from Cacahuacintle Maize

Fernández-Aulis

Hérnández-Vázquez

Aguilar-Osorio

et al. 2019

Journal of Food Science

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Pigmented maize has been extensively studied due to its high anthocyanin content. This study has been focused mainly on kernel, although the whole plant of purple corn is a potential source of anthocyanins. First, general parameters of extraction (solvent system, solvent-to-solid ratio, number of extractions, and acid type) were established depending on the total anthocyanins content. Then, three extraction methods to access anthocyanins were compared: maceration extraction (ME), ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE). Since the residual material still possessed an intense color, a further treatment was performed by application of enzymatic-assisted extraction (EAE). Three enzymatic cocktails (Xylanases, Celluclast, and Depol), pH, and temperature were evaluated to establish optimal reaction conditions. Subsequent analysis and identification of the anthocyanins obtained by four different extraction techniques were performed using HPLC and HPLC-mass spectrometry, respectively. The most efficient method was UAE using 20 min of ultrasound (100 W) preceded by sample treatment in the following conditions: ethanol/water/lactic acid mixture (80:19:1), two extractions, 1:10 solvent-to-solid ratio. As a result, anthocyanins from corn cob and corn husk were extracted at concentrations of 24.32 and 25.80 mg/gDW, respectively. No difference in the anthocyanins profile for samples extracted by three different methods was observed. However, an enhanced presence of cyanidin-3-(6''malonyl)glucoside was detected in the sample corresponding to the EAE method. Therefore, the Cahuacintle corn husk can be considered as a competitive source of anthocyanins with the available commercial sources.

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“…They have capability to reduce feed viscosities which directly increase the nutrients absorption capability of animals. These nutrients are released from fibers using hydrolysis process and it also reduces animal defecation [13,[79][80][81][82][83][84].…”

Section: Pectinasesmentioning

confidence: 99%

Prospective Application ofAspergillusSpecies: Focus on Enzyme Production Strategies, Advances and Challenges

Gholami-Shabani¹,

Shams-Ghahfarokhi²,

Jamzivar³

et al. 2022

Natural Food Additives

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Fungal enzymes that catalyze different types of biochemical reactions play a significant role in modern industry by improving existing processes. Also, the use of enzymes to replace some traditional toxic chemical or mechanical approaches helps decrease energy demand and environmental pollution. However, enzymes must be able to compete commercially with relatively low-priced traditional approaches. Meeting economical and commercial feasibility criteria depends on a number of enzymatic properties including the specificity to the substrate, stability in industrial enzymatic reaction conditions and catalytic efficiency. Fungi used as an enzyme manufacture host should be appropriate for industrial scale fermentation. Aspergillus species are being developed as one of the best enzyme manufacture factories due to their capability to secrete high quantities of enzymes suitable for industrial applications. The industrial importance of Aspergillus species also includes the progress and commercialization of new products derived from genetically engineered modified strains. Hence, the main aim of this chapter investigation is to analyze the secreted and cellular proteins from Aspergillus species and their application in industries.

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Scaling-up and ionic liquid-based extraction of pectinases from Aspergillus flavipes cultures

Cited by 34 publications

References 35 publications

Choline dihydrogen phosphate-based deep eutectic solvent: A suitable bioplatform for lipase extraction

Choline dihydrogen phosphate-based deep eutectic solvent: A suitable bioplatform for lipase extraction

Extraction and Identification of Anthocyanins in Corn Cob and Corn Husk from Cacahuacintle Maize

Prospective Application ofAspergillusSpecies: Focus on Enzyme Production Strategies, Advances and Challenges

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