Scanning acoustic microscopy for characterization of neoplastic and inflammatory lesions of lymph nodes

Miura, Katsutoshi; Nasu, Hatsuko; Yamamoto, Seiji

doi:10.1038/srep01255

Cited by 46 publications

(30 citation statements)

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“…At a frequency approximately higher than 300 MHz, individual cells can be resolved while at a frequency ranging from 1 to 1.5 GHz detailed information about their internal structure becomes apparent. [21][22][23] Liver carcinoma, gastric cancer, lesions of lymph nodes, breast and renal cancer were actively investigated by many research and groups 16,[24][25][26][27] showing that tumour tissues exhibit distinct sound speed and attenuation comparing to healthy tissues. The magnitude and indication of these pathological modification change for various tumour.…”

Section: Introductionmentioning

confidence: 99%

High‐resolution quantitative acoustic microscopy of cutaneous carcinoma and melanoma: Comparison with histology

Youssef

Seviaryna

Shum

et al. 2019

Skin Research and Technology

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Background The increased incidence rate of skin cancers during the last decades is alarming. One of the significant difficulties in the histopathology of skin cancers is appearance variability due to the heterogeneity of diseases or tissue preparation and staining process. This study aims to investigate whether the high‐resolution acoustic microscopy has the potential for identifying and quantitatively classifying skin cancers. Material/Methods Unstained standard formalin‐fixed skin tissue samples were used for ultrasonic examination. The high‐frequency acoustic microscope equipped with the 320 MHz transducer was utilized to visualize skin structure. Fourier transform was performed to calculate the sound speed and attenuation in the tissue. Results The acoustic images demonstrate good concordance with the traditional histology images. All histological features in the tumour were easily identifiable on acoustic images. Each skin cancer type has its combination of ultrasonic properties significantly different from the healthy skin. Conclusions High‐resolution acoustic imaging strengthened with quantitative analysis shows a potential to work as an auxiliary imaging modality assisting pathologists to lean to the particular decision in doubtful cases. The method can also assist surgeon to ensure the complete resection of a tumour.

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Section: Introductionmentioning

confidence: 99%

High‐resolution quantitative acoustic microscopy of cutaneous carcinoma and melanoma: Comparison with histology

Youssef

Seviaryna

Shum

et al. 2019

Skin Research and Technology

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“…Thyroid lymphomas showed a faster speed compared with lymphomas in other organs. 5,6 Fibrous capsules or bands around carcinomas were split (Figures 6 and 8-10), and surrounding follicles were angular or irregular with fragmented colloids.…”

Section: Resultsmentioning

confidence: 99%

“…SOS compared with lymphomas of other organs such as the stomach 5 and lymph nodes. 6 Rapidly growing tumors, such as the undifferentiated or poorly differentiated carcinomas, showed the slowest SOS, probably because of collagen-deficient stroma. From previous observations, carcinomas had slow SOS if few desmoplastic reactions occurred.…”

Section: Discussionmentioning

confidence: 99%

“…3 SAM uses US at 120 MHz with almost the same resolution (approximately 12.5 µm) as the low magnification of a light microscope (LM). The authors recently reported that SAM could be used to generate useful information on the lung, 4 stomach, 5 and lymph node 6 lesions. To date, no reports have been found on the application of SAM in thyroid histology.…”

Section: Introductionmentioning

confidence: 99%

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Histological evaluation of thyroid lesions using a scanning acoustic microscope

Miura¹,

Mineta²

2014

PLMI

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Video abstractPoint your SmartPhone at the code above. If you have a QR code reader the video abstract will appear. Or use: http://dvpr.es/1et29bE Dovepress submit your manuscript | www.dovepress.com Dovepress 1 O r I g I n a L r e s e a r c h open access to scientific and medical research Open access Full Text article http://dx.Purpose: A scanning acoustic microscope (SAM) uses an ultrasound to image an object by plotting the speed-of-sound (SOS) through tissues on screen. Because hard tissues result in great SOS, SAM can provide data on the tissue elasticity. This paper investigated the utility of SAM in evaluating thyroid lesions. Methods: Formalin-fixed, paraffin sections were scanned with a 120 MHz transducer. SOS through each area was calculated and plotted on the screen to provide histological images, and SOS of each lesion was compared and statistically analyzed. Results: High-concentrated colloids, red blood cells, and collagen fibers showed great SOS, while low-concentrated colloids, parathyroids, lymph follicles, and epithelial tissues including carcinomas demonstrated lower SOS. SAM clearly discriminated structure of thyroid components corresponding to low magnification of light microscopy. Thyroid tumors were classified into three groups by average SOS: the fast group consisted of follicular adenomas/carcinomas and malignant lymphomas; the slow group contained poorly differentiated/undifferentiated carcinomas; and the intermediate group comprised papillary/medullary carcinomas. Fragmented colloids, irregular-shaped follicles, and desmoplastic reactions were observed in the invasive area of surrounding carcinomas. Conclusion: The SAM imaging method had the following benefits: 1) precise images were acquired in a few minutes without special staining; 2) structural irregularity and desmoplastic reactions, which indicated malignancy, were detected; 3) images reflected tissue elasticity, which was statistically comparable among lesions by SOS; 4) follicular functional activity was predictable by converting colloid concentration to SOS; and 5) tumor classification was predictable by SOS because more poorly differentiated carcinomas had a tendency to show lower SOS.

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“…SAM has been applied successfully for characterizing various soft tissues and disease states [8]- [12]. However, only one study reported SAM measurements for human LN tissues [10]. The authors studied the distribution of speed of sound (c) of various tissue types with a resolution of 13 μm using a 120-MHz transducer.…”

Section: Introductionmentioning

confidence: 99%

Fine-resolution maps of acoustic properties at 250 MHz of fresh samples and unstained fixed 12-µm thin sections from cancerous human lymph nodes

Rohrbach

Saegusa‐Beecroft

Yanagihara

et al. 2014

2014 IEEE International Ultrasonics Symposium

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Scanning acoustic microscopy (SAM) at 250 MHz permits measuring the acoustic properties of tissue microstructure with a spatial resolution of 7 µm. However, only limited data exist for sentinel lymph nodes at these scales. 250-MHz SAM was performed on four fixed and one fresh lymphnode samples. 12-µm sections of deparaffinized, fixed tissue and fresh half nodes were scanned using a custom-built acoustic microscope. 2D SAM maps of speed of sound (c), attenuation (α), and acoustic impedance (Z) were generated using custom signal processing algorithms. Scanned samples then were stained using hematoxylin and eosin and imaged by light microscopy. The spatial resolution and contrast of SAM maps were sufficient to distinguish among tissue regions consisting of lymphocytes, fat cells, and fibrous tissue. Average properties for lymphocyte-rich tissue were c = 1575 ± 57 m/s, α = 8.27 ± 2.45 dB/MHz/cm, and Z = 1.59 ± 0.14 Mrayl. We found a significant increase (p<0.05, ANOVA) of 69.0 ± 0.5 m/s for c and 1.2 ± 0.1 dB/MHz/cm for α in fibrous connective tissue compared to lymphocyte-rich tissue. The study demonstrates that fine-resolution maps of acoustic properties of lymph nodes can be generated at 250 MHz. The data will serve as a basis for developing new lymph-node-specific ultrasound-scattering models to improve current quantitative ultrasound approaches for detecting metastatic regions in freshly-excised sentinel lymph nodes from breast-cancer patients.

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Scanning acoustic microscopy for characterization of neoplastic and inflammatory lesions of lymph nodes

Cited by 46 publications

References 9 publications

High‐resolution quantitative acoustic microscopy of cutaneous carcinoma and melanoma: Comparison with histology

High‐resolution quantitative acoustic microscopy of cutaneous carcinoma and melanoma: Comparison with histology

Histological evaluation of thyroid lesions using a scanning acoustic microscope

Fine-resolution maps of acoustic properties at 250 MHz of fresh samples and unstained fixed 12-µm thin sections from cancerous human lymph nodes

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Scanning acoustic microscopy for characterization of neoplastic and inflammatory lesions of lymph nodes

Cited by 46 publications

References 9 publications

High‐resolution quantitative acoustic microscopy of cutaneous carcinoma and melanoma: Comparison with histology

High‐resolution quantitative acoustic microscopy of cutaneous carcinoma and melanoma: Comparison with histology

Histological evaluation of thyroid lesions using a scanning acoustic microscope

Fine-resolution maps of acoustic properties at 250 MHz of fresh samples and unstained fixed 12-&#x00B5;m thin sections from cancerous human lymph nodes

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Product

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Fine-resolution maps of acoustic properties at 250 MHz of fresh samples and unstained fixed 12-µm thin sections from cancerous human lymph nodes