Scanning electrochemical microscopy with enzyme immunoassay of the cancer-related antigen CA15-3

Zhang, Xiaoli; Peng, Xuewei; Jin, Wenrui

doi:10.1016/j.aca.2005.11.032

Cited by 74 publications

(18 citation statements)

References 22 publications

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“…Eine Reihe von Immunassays mit SECMDetektion wurde realisiert. [340,345,[391][392][393][394][395] Die Abbildung 21 zeigt exemplarisch einen Zweifachimmunassay für humanes Plazentalactogen (HPL) und humanes Chorionogonadotropin (HCG). [345] Der Analyt ist durch die Position auf dem Chip bestimmt, während das UME-Signal an der jeweiligen Position eine Quantifizierung ermöglicht.…”

Section: Enzymmarkierungenunclassified

Elektrochemische Rastermikroskopie zur direkten Abbildung von Reaktionsgeschwindigkeiten

Wittstock¹,

Burchardt²,

Pust³

et al. 2007

Angewandte Chemie

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Eine entscheidende Rolle in der Elektrochemie, aber auch in biologischen Systemen oder beim Transport durch Membranen, spielen lokale Prozesse an Fest‐flüssig‐Grenzflächen oder Flüssig‐flüssig‐Grenzflächen, die z. B. an Defektstellen, an Poren oder an einzelnen Zellen ablaufen und sich durch integrale Untersuchungsverfahren nur schwer erfassen lassen. Mit der elektrochemischen Rastermikroskopie steht eine Untersuchungsmethode für lokale Grenzflächenreaktionen zur Verfügung, die nach zwei Jahrzehnten Entwicklung eine solide theoretische Basis und eine große Zahl von Anwendungen aufweist. Sie bietet die Möglichkeit, Geschwindigkeiten heterogener Reaktionen direkt abzubilden und Oberflächen durch elektrochemisch erzeugte Reaktanten lokal zu modifizieren. Die Vielfalt der Anwendungen reicht von klassischen elektrochemischen Fragestellungen bei der Untersuchung lokaler Korrosionsphänomene und elektrokatalytischer Reaktionen in Brennstoffzellen, von Sensoroberflächen, Biochips und mikrostrukturierten Analysesystemen bis hin zum Massentransport durch synthetische Membranen, Haut und Gewebe sowie interzellulären Kommunikationsprozessen. Außerdem können Prozesse an Flüssigkeitsoberflächen oder an Flüssig‐flüssig‐Grenzflächen untersucht werden.

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Section: Enzymmarkierungenunclassified

Elektrochemische Rastermikroskopie zur direkten Abbildung von Reaktionsgeschwindigkeiten

Wittstock¹,

Burchardt²,

Pust³

et al. 2007

Angewandte Chemie

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“…Many methods have been developed for the determination of CA15-3 levels including immunoradiometric assay [11,12], enzymelinked immunoassay [13,14], microparticle enzyme immunoassay [14,15], chemiluminescence immunoassay [14], immunofluorometric assay [16,17], optical protein-chip test [18], electrochemiluminescence immunoassay [18], and capillary electrophoresis enzyme immunoassay with electrochemical (EC) detection [19]. CE, with high separation efficiency, short analysis time, and minimum consumption of sample and reagents, can rapidly separate macromolecules such as proteins and immunocomplexes [20].…”

Section: Introductionmentioning

confidence: 99%

Sensitive detection of tumor marker CA15‐3 in human serum by capillary electrophoretic immunoassay with chemiluminescence detection

Liu¹,

Zheng

Cao

et al. 2008

J of Separation Science

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A new and sensitive non-competitive immunoassay (IA) for tumor marker carbohydrate antigen 15-3 (CA15-3) by CE coupling with ECL detection has been developed. This method is based on luminol-H(2)O(2 )reaction catalyzed by horseradish peroxidase (HRP). The optimum CE separation and CL detection conditions were investigated. After the non-competitive immunoreaction, the free HRP-labeled CA15-3 antibody (Ab*) and the bound Ab*-antigen (Ab*-Ag) complex were separated in a separation capillary and then catalyzed the CL reaction of luminol and H(2)O(2 )in a reaction capillary following the separation capillary. The calibration curve based on the peak areas of Ab*-Ag complex plotted against the concentrations of CA15-3 is in the range of 0-250 U/mL with a correlation coefficient of 0.9983 and the detection limit is 0.035 U/mL (S/N = 3). The response for five consecutive injections of 125 U/mL CA15-3 resulted in RSDs of 0.83% and 3.1% for the migration time and the peak area, respectively. The method was successfully used for the quantification of CA15-3 in human sera obtained from healthy persons and from patients with breast cancer.

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“…[22] Imaging of patterns of surfaceimmobilized enzymes using SECM is important to the characterization and optimization of enzyme-based sensors [23] and for the formation of biosensors, for example, for the detection of breast cancer markers. [24] An attraction of the SECM approach is that the enzymes do not have to be immobilized on an electrode surface, although they may be, [10a] thereby allowing ET kinetics to be investigated in a range of interfacial environWe report the use of evanescent wave cavity ring-down spectroscopy (EW-CRDS) to monitor the reduction by ethylenediaminetetraacetic acid iron(II) complex, [FeEDTA] 2À , of an adsorbed layer of oxidized cytochrome c immobilized on fused silica. The adsorption of cytochrome c at the silica-water interface was also probed using EW-CRDS and found to be in qualitative agreement with previous studies.…”

Section: Introductionmentioning

confidence: 97%

Probing Redox Reactions of Immobilized Cytochrome c Using Evanescent Wave Cavity Ring‐Down Spectroscopy in a Thin‐Layer Electrochemical Cell

et al. 2010

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We report the use of evanescent wave cavity ring-down spectroscopy (EW-CRDS) to monitor the reduction by ethylenediaminetetraacetic acid iron(II) complex, [FeEDTA](2-), of an adsorbed layer of oxidized cytochrome c immobilized on fused silica. The adsorption of cytochrome c at the silica-water interface was also probed using EW-CRDS and found to be in qualitative agreement with previous studies. The reduction of the adsorbed cytochrome c was achieved by using a strategically positioned electrode to electrogenerate FeEDTA(2-), which diffused to the silica surface and reduced the cytochrome c. The difference in the absorption spectra of the reduced and oxidized forms of cytochrome c at 400 nm allowed the direct monitoring of the electron transfer in real time. Using finite-element modelling, the rate constant of electron transfer (ET) between FeEDTA(2-) and cytochrome c was found to be 4.3(± 0.6) × 10(-9) cm s(-1) equivalent to 2.7(± 0.4) M(-1) s(-1). The latter value is considerably lower than previously reported ET rate constants between cytochrome c and FeEDTA(2-) in solution, which can be attributed to the confinement of the immobilized cytochrome c on the surface and possible effects from molecular crowding. This study highlights the importance of new methods which can be used to study ET at interfaces and opens up the possibility of studying ET to proteins in biologically relevant environments using EW-CRDS.

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Scanning electrochemical microscopy with enzyme immunoassay of the cancer-related antigen CA15-3

Cited by 74 publications

References 22 publications

Elektrochemische Rastermikroskopie zur direkten Abbildung von Reaktionsgeschwindigkeiten

Elektrochemische Rastermikroskopie zur direkten Abbildung von Reaktionsgeschwindigkeiten

Sensitive detection of tumor marker CA15‐3 in human serum by capillary electrophoretic immunoassay with chemiluminescence detection

Probing Redox Reactions of Immobilized Cytochrome c Using Evanescent Wave Cavity Ring‐Down Spectroscopy in a Thin‐Layer Electrochemical Cell

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