Scanning Electron Microscope Studies of the Shoot Apex in Dianthus caryophyllus L. cv. Scania1

Abstract

Intact shoot apex development of the carnation was studied with a scanning electron microscope using fresh tissue. Reproductive meristems were more resistant to desiccation from the hard vacuum and electron beam than vegetative meristems. Vegetative meristems, however, can be studied if the work is done quickly. Structural changes which occurred in the developing carnation shoot showed that leaf primordia are initiated in a circular whorl. Floral initiation was easily determined by the appearance of a flattened apex and a pentagonal whorl of sepal primordia. Subsequent centripetal initiation of flower parts was easily recognized and identified.

Scanning Electron Microscope Examination of Flower Bud Differentiation in Sour Cherry1

“…ACP leaves differ anatomically from those of greenhouse plants (2). ACP leaves may have less epicuticular wax (4,6), smaller palisade cells (2), and more mesophyll air space (2). The water stress of high RH plants when transferred to a lower RH has…”

Electron Optics: Principles, Techniques, and Applications in Horticulture1

“…The organogenesis of meristems by SEM analysis gives a total picture of meristimatic activity and morphology (36). The lily (32) and carnation (33) developmental stages have been clearly identified and characterized. Mutational alteration of the reproductive apex in carnation (33) has led to a better understanding of the sequence of events in carnation flower production.…”

Floral Development and Blindness in Roses: An SEM Study1

“…(3) and Emino and Rasmussen (5), on apical development in the Easter lily and the carnation, illustrated the value of the scanning electron microscope (SEM) in increasing the depth of field and providing a means of viewing and photographing the entire apex as well as reducing sample preparation time.…”