Scanning electron microscopy of the corneal endothelium of ostrich

Pigatto, João Antônio Tadeu; Franzen, Ângela Aguiar; Pereira, Fabiana Quartiero; Almeida, Ana Carolina da Veiga Rodarte de; Laus, José Luis; Santos, Jaime Maia dos; Guedes, Pedro Mancini; Barros, Paulo Sérgio de Moraes

doi:10.1590/s0103-84782009005000001

Cited by 23 publications

(28 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the current study, there was no significant difference between the right and left eyes. There was no difference in relation to endothelial parameters obtained between fellow eyes of the same individual (MORITA, 1985;ANDREW et al, 2001;PIGATTO et al, 2004;PIGATTO et al, 2005b;PIGATTO et al, 2008;PIGATTO et al, 2009;FRANZEN et al, 2010).…”

Section: Discussionmentioning

confidence: 88%

“…The high cost of specular microscopes and the difficulty in obtaining good images in injured endothelial areas are common challenges or barriers to the use of this technique (ANDREW et al, 2001;PIGATTO et al, 2005a;SAAD et al, 2008). SEM has been widely used to compare the endothelial ultrastructure of vertebrates and to evaluate the effects of medications, chemicals, or surgical procedures on the endothelium (COLLIN & COLLIN, 1998;PIGATTO et al, 2004;PIGATTO et al, 2005a;PIGATTO et al, 2005b;PIGATTO et al, 2009). Changes in endothelial cell dimensions can occur as a result of processing the cornea for SEM and the data obtained with regard to endothelial cell density cannot be compared with those obtained using specular microscopy (SCHUTTEN & VAN HORN , 1980).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Morphology of endothelial cells from different regions of the equine cornea

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Morphology of endothelial cells from different regions of the equine cornea

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…Among the limitations of specular microscopy, difficulties in obtaining images in corneas with oedema and in capturing images of peripheral regions of the cornea have been mentioned [19]. When the cornea is not transparent, in vitro techniques have been employed for endothelial analysis, achieving good results [11][12][13]16,24]. In the present study, coloration and optical microscopy enabled the analysis, photography and the study of the shape of the endothelium in all regions of the cornea.…”

Section: Discussionmentioning

confidence: 84%

Morphology of Endothelial Cells from Different Regions of the Swine Cornea

Clerot

Hünning

Bettio

et al. 2019

Acta Scientiae. Vet.

Self Cite

View full text Add to dashboard Cite

Background:The corneal endothelium is a monolayer of polygonal cells which constitute the last layer of the cornea. The integrity of this layer is critical to cornea transparency. The characterization of normal corneal endothelial morphology is important not only to clinical evaluation but also to selection of areas of the cornea with better quality to be employed as donor tissue. The aim of the present study was to evaluate the morphology of endothelial cells from different regions of the swine cornea after alizarin red staining using optical microscopy. Materials, Methods & Results:Twenty-four healthy eyes from 12 swine Large White breed, with 14-monthold, males or females obtained from a licensed Brazilian commercial slaughterhouse were studied. Immediately after humane slaughter, the eyes were enucleated and submitted to ophthalmic examination. Eyes with signs of diseases of the anterior segment were excluded. The cornea, with 3 mm of the sclera, was removed and placed on a glass microscope slide with the endothelial side up. Four radial incisions were made in the periphery of the cornea to better accommodate the cornea on the microscope slide. Alizarin red was diluted in isotonic solution (0.2 g/100 mL) and the pH was adjusted to 4.2 with hydrochloric acid. Three drops of alizarin red were placed on the corneal endothelium. After 90 s, the dye was removed from the cornea with balanced saline solution. The corneal endothelium was examined and photographed using an optical microscope. All evaluations were performed by the same investigator. Photomicrographs were taken of central, superior, inferior, nasal and temporal corneal areas. Parameter studied included endothelial cell morphology. For the statistical analysis, was employed the ANOVA variance test (repeated measures). Differences were considered statistically significant at P < 0.05. Normal endothelium cells were mainly hexagonal (83.7%), pentagonal (7.45%) and heptagonal (8.8%), with a minimal number of cells of other shapes present. There were no significant statistical differences in the proportion of the morphology and the different regions of the cornea (P = 0.31). Discussion: Different techniques are available for the analysis of corneal endothelium, including mainly scanning electron microscopy, specular microscopy and optical microscopy. The analysis of the morphology of corneal endothelium with an optic microscope after staining with alizarin red has been described as an effective, rapid and cost-efficient method, since this dye blends the borated cells, allowing identification. In the present study, using optical microscopy and coloration with alizarin red it was possible to explore and to obtain images of the swine endothelium of all regions of the cornea. The analysis of the cellular morphology or the percentage of hexagonal cells are among the main parameters used to evaluate the health of the corneal endothelium. In this study, the endothelium had the predominance of the hexagonal shape in all regions studied. In swine, there are no studies...

show abstract

“…Specular microscopy, optical microscopy, and scanning electron microscopy (SEM) are among the most widely used methods for corneal endothelium analysis (MORITA et al, 1994;PIGATTO et al, 2009;ALBUQUERQUE et al, 2015;COYO et al, 2016;FAGANELLO et al, 2016). There is consensus that the preparation of corneas for studies using SEM causes cell retraction by reducing the original cell area and increasing the endothelial cell density (VIRTANEN et al, 1984;FAGANELLO et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, SEM has been used to study the ultrastructure of endothelial cells of different species of animals (PIGATTO et al, 2004;PIGATTO et al, 2005a;PIGATTO et al, 2005b;PIGATTO et al, 2009;TAMAYO-ARANGO et al, 2009). Due to the fact that there is no cellular retraction and it can be used in animals and living humans, specular microscopy is considered the gold standard technique for assessing the corneal endothelium in people and animals (MORITA, 1995;ABIB & BARRETO, 2001;PIGATTO et al, 2006;PIGATTO et al, 2008;FRANZEN et al, 2010;BERCHT et al, 2015;ALBUQUERQUE et al, 2015;COYO et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Corneal endothelial cell density and morphology in rabbits’ eyes using contact specular microscopy

et al. 2017

Self Cite

View full text Add to dashboard Cite

The goal of this study was to describe the normal values of corneal endothelial cell density and hexagonality in healthy rabbits’ eyes using contact specular microscopy. Both eyes of 18 rabbits, males and females, of different ages were evaluated. The eyes were divided into three groups of 12 each according to the age range of the animals: G1 (6 months old), G2 (12 months old), and G3 (48 months old). Parameters studied included mean endothelial cell density and hexagonality. For Group I, the mean cell density was 2307 cells per mm2. For GII, the mean cell density was 1895 cells per mm2. For G III, the mean cell density was 1818 cells per mm2. Cell density decreased significantly with aging (P<0.001) among the three groups. Pleomorphism in the rabbits of GI was 74.33±10.08. In the rabbits of GII it was 71.83±11.38. In the rabbits of GIII it was 64.02±28.80. Significant differences (P<0.001) were evident between GI and GIII and GII and GIII but not between Groups I and II. The results showed a decrease in number of cells and hexagonality with aging. Corneal endothelium of rabbits suffers changes due to advancing age both in terms of cell density and morphology.

show abstract

Scanning electron microscopy of the corneal endothelium of ostrich

Abstract: The aim of this study was to examine the endothelial surface morphology and perform a morphometric analysis of the corneal endothelial cells of ostrich (Struthio

Cited by 23 publications

References 13 publications

Morphology of endothelial cells from different regions of the equine cornea

Morphology of endothelial cells from different regions of the equine cornea

Morphology of Endothelial Cells from Different Regions of the Swine Cornea

Corneal endothelial cell density and morphology in rabbits’ eyes using contact specular microscopy

Contact Info

Product

Resources

About