Scanning Probe Nanolithography and Protein Patterning of Low‐Fouling Plasma Polymer Multilayer Films

Muir, Benjamin W.; Fairbrother, Andrew; Gengenbach, Thomas R.; Rovere, Florian; Abdo, Michael A.; McLean, Keith M.; Hartley, Patrick G.

doi:10.1002/adma.200600343

Cited by 52 publications

(52 citation statements)

References 39 publications

(38 reference statements)

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“…Therefore patterning methods are required that offer functionalization and/or passivation of surfaces on a nanometer scale. Muir and co-workers have shown that protein patterns can be elegantly fabricated by using scanning probe nanolithography [9]. In their study a two-layered system prepared by a plasma deposition process was used.…”

Section: Introductionmentioning

confidence: 99%

Interface roughness of plasma deposited polymer layers

Zhang

Arfsten

Pihan

et al. 2010

Journal of Colloid and Interface Science

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Section: Introductionmentioning

confidence: 99%

Interface roughness of plasma deposited polymer layers

Zhang

Arfsten

Pihan

et al. 2010

Journal of Colloid and Interface Science

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“…One of the most common classes of thin film treatments employed in biomaterial devices is that of 'low-fouling' or 'stealth' coatings [6]. These coatings need to be able to resist or inhibit protein adsorption within the body.…”

Section: Introductionmentioning

confidence: 99%

An X-ray and neutron reflectometry study of ‘PEG-like’ plasma polymer films

Menzies

Nelson

Shen

et al. 2011

J. R. Soc. Interface.

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Plasma-enhanced chemical vapour-deposited films of di(ethylene glycol) dimethyl ether were analysed by a combination of X-ray photoelectron spectroscopy, atomic force microscopy, quartz crystal microbalance with dissipation monitoring (QCM-D), X-ray and neutron reflectometry (NR). The combination of these techniques enabled a systematic study of the impact of plasma deposition conditions upon resulting film chemistry (empirical formula), mass densities, structure and water solvation, which has been correlated with the films' efficacy against protein fouling. All films were shown to contain substantially less hydrogen than the original monomer and absorb a vast amount of water, which correlated with their mass density profiles. A proportion of the plasma polymer hydrogen atoms were shown to be exchangeable, while QCM-D measurements were inaccurate in detecting associated water in lower power films that contained loosely bound material. The higher protein resistance of the films deposited at a low load power was attributed to its greater chemical and structural similarity to that of poly(ethylene glycol) graft surfaces. These studies demonstrate the utility of using X-ray and NR analysis techniques in furthering the understanding of the chemistry of these films and their interaction with water and proteins.

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“…Unlike other SPL methods, DPN bypasses the need to initially alter the intended surface either physically or chemically before deposition of the biomolecules and instead directly deposits the biomolecule “ink” onto the surface (Salaita, Wang, & Mirkin, 2007). Alternative SPL methods have also been described where regions of a non-fouling surface are “dynamically plowed” off through the use of an AFM tip, thus exposing the scratched off zones to protein adsorption (Muir, Fairbrother, Gengenbach, Rovere, Abdo, McLean et al , 2006). Although these protein-compatible technologies allow for resolutions down to tens of nanometers, the techniques suffer from very low throughput and thus present a multitude of challenges when considering the scalability of the process.…”

Section: Introductionmentioning

confidence: 99%

High Fidelity Nanopatterning of Proteins onto Well-Defined Surfaces Through Subtractive Contact Printing

García

Singh

Garcı́a

2014

Methods in Cell Biology

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In the pursuit to develop enhanced technologies for cellular bioassays as well as understand single cell interactions with its underlying substrate, the field of biotechnology has extensively utilized lithographic techniques to spatially pattern proteins onto surfaces in user-defined geometries. Microcontact printing (μCP) remains an incredibly useful patterning method due to its inexpensive nature, scalability, and the lack of considerable use of specialized clean room equipment. However, as new technologies emerge that necessitate various nano-sized areas of deposited proteins, traditional microcontact printing methods may not be able to supply users with the needed resolution size. Recently, our group developed a modified “subtractive microcontact printing” method which still retains many of the benefits offered by conventional μCP. Using this technique, we have been able to reach resolution sizes of fibronectin as small as 250 nm in largely spaced arrays for cell culture. In this communication, we present a detailed description of our subtractive μCP procedure that expands on many of the little tips and tricks that together make this procedure an easy and effective method for controlling protein patterning.

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Scanning Probe Nanolithography and Protein Patterning of Low‐Fouling Plasma Polymer Multilayer Films

Cited by 52 publications

References 39 publications

Interface roughness of plasma deposited polymer layers

Interface roughness of plasma deposited polymer layers

An X-ray and neutron reflectometry study of ‘PEG-like’ plasma polymer films

High Fidelity Nanopatterning of Proteins onto Well-Defined Surfaces Through Subtractive Contact Printing

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