Scanning (SEM) and transmission (TEM) electron-microscopic studies on post-ischemic endothelial lesions following recirculation

Elemér, G; Kerényi, T; Jellinek, H

doi:10.1016/0021-9150(76)90077-0

Cited by 20 publications

(10 citation statements)

References 12 publications

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“…The lesion typically regresses over 24-48 h (1). This time-course suggests "healing" and normalization of injured endothelial cells, rather than replacement of a population of irreparable or dead cells (49). Our data show that ACE is released by lung endothelium under base-line conditions and after injury, and that release is not simply an indicator of cell death.…”

Section: Methodsmentioning

confidence: 68%

Release of angiotensin converting enzyme by the lung after Pseudomonas bacteremia in sheep.

Gorin¹,

Hasagawa²,

Hollinger³

et al. 1981

J. Clin. Invest.

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A B S T R A C T We studied release of angiotensin-converting enzyme (ACE) by the lung after acute injury associated with an increase in pulmonary vascular permeability. In eight adult sheep with chronic lung lymph fistulas, we measured lymph flow (QL), and both ACE activity and total protein content in lymph and plasma under base-line conditions and during 24 h after an infusion of live pseudomonas organisms.Under base-line conditions, ACE activity in plasma was 4.93+0.43 U/ml (mean±SEM ml/h before pseudomonas bacteremia, and rose to 6.7 ± 1.2 ml/h by 2 h after onset of the infusion (mean ±SEM, P < 0.05). CTP remained significantly elevated for at least 10 h after the infusion. Release of ACE into lung lymph doubled after acute lung injury and equaled 22.3 ± 13.8 U/h at 4 h after onset of the infusion. ACE secretion into lung lymph had returned to baseline levels by 24 h after bacteremia. We did not observe a significant rise in plasma ACE activity after acute lung injury.Pseudomonas bacteremia in sheep results in acute, reversible lung injury associated with increased pulmonary vascular permeability, and increased release of Address reprint requests to Dr.

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Section: Methodsmentioning

confidence: 68%

Release of angiotensin converting enzyme by the lung after Pseudomonas bacteremia in sheep.

Gorin¹,

Hasagawa²,

Hollinger³

et al. 1981

J. Clin. Invest.

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“…The necessary pressure data are, therefore, found more frequently in recent studies (Bjorkerud et al 1972, Buss et al 1973, Buss and Hollweg 1977, Buss et al 1976, Bylock et al 1977, DeBruijn and Van Mourik 1975, Elemer et al 1976, Fishman et al 1975, Hollweg et al 1978, Mayberg et al 1978, Reidy and Bowyer 1977, Shimamoto 1974, Svendsen and Jorgensen 1978, Swinehart et al 1976, Thurston et al 1976). However, where and how the pressure levels were ascertained is all too seldom explained in detail.…”

Section: Perfusion Pressurementioning

confidence: 90%

“…In recent years the perfusion method has prevailed more and more (Buss et al 1973, Buss and Hollweg 1977, Buss et al 1976, DeBruijn and VanMourik 1975, Edanaga 1974, Elemer et al 1976, Fishman et al 1975, Fonkalsrud et al 1976, Frenzel et al 1976, Hess and Staubli 1963, Shimamoto 1974, Svendsen and Jorgensen 1978, Webster et al 1974). Unfortunately there are also additional methodological variations and possibilities of error involved in this more complicated technique, making comparisons more difficult.…”

Section: Fixation Immersion or Perfusion Fixation?mentioning

confidence: 99%

“…In most cases a follow-up fixation by immersion occurs after the perfusion fixation and killing. It is difficult to comprehend why the fixative used in the second stage of fixation (other than a post-osmication for TEM) often has a different composition and concentration (Elemer et al 1976, Fishman et al 1975, Kawamura et al 1974) than the fixative used for perfusion.…”

Section: Fixation Immersion or Perfusion Fixation?mentioning

confidence: 99%

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Problems with the preparation of blood vessels for scanning electron microscopy a critical review

Hollweg

Buss

1980

Scanning

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“…[5][6][7][8] The literature is vast in its documentation of physiological alterations due to the interruption of the arterial flow. However, little is known about its effects on the normal arterial endothelium morphology, such as those which occur in the normal arterial port [9][10][11][12] Purpose Analyze the effects of the interruption of the flow in the normal segmental arterial endothelium morphology to the area suffering acute arterial occlusion dute to emboly as well as correlate them to ischemia duration.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the effects of the interruption of the flow in the normal arterial endothelium morphology and the correlation with the ischemia duration in rabbits

et al. 2007

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The study of acute arterial insufficiency of the extremities is an area of continuing interest and investigation, in light of the possible effects stemming from the evolution of the disease and the necessity for urgent treatment. Purpose: To analyze the effects of the interruption of the flow in the normal arterial endothelium morphology and correlate them with the ischemia duration. Methods: We submitted 30 rabbits to the ligature of the right external iliac artery for 6 hours or 72 hours and observed specific morphological variables in the endothelial layer under optical and electronic microscopy. Results: In the optical microscopic study, no statistically significant results were observed in the comparison of the groups (control, 6-and 72-hour occlusions). With electronic microscopy, we observed alterations in the endothelial cell characterized by hyperpigmentation with detachment of the same from its bed; and rupture of the internal elastic membrane, with the exposure of the subendothelial material to the vascular lumen. Conclusions: The optical microscopy was not an effective method for the determination of endothelial morphological alterations; the electronic microscopy allowed us to observe initial signals of the endothelial cell and layer injury 72 hours after the interruption of the normal arterial flow. Key words: Endothelium. Microscopy, electron. Ischemia. Rabbits. RESUMOO estudo da insuficiência arterial aguda das extremidades é área de contínuo interesse e investigação, devido possibilidade de eventos catastróficos na evolução da doença e necessidade de tratamento cirúrgico de urgência. Objetivo: Analisar os efeitos da ausência de fluxo na morfologia do endotélio arterial normal segmentar, como os que ocorrem na porção imediatamente abaixo da área que sofreu oclusão arterial aguda por embolia, e correlacioná-los com o tempo de isquemia. Métodos: Submetemos 30 coelhos à ligadura da artéria ilíaca externa direita por 6 horas ou 72 horas e observamos variáveis morfológicas específicas da camada endotelial e subendotelial à microscopia óptica e eletrônica. Resultados: No estudo da microscopia óptica não foram observados resultados estatisticamente significativos quando comparados os grupos entre si. À microscopia eletrônica observamos alterações da célula endotelial caracterizadas por hiperpigmentação e descolamento da mesma de seu leito, e ruptura da membrana elástica interna com exposição do material subendotelial para luz vascular. Conclusões: A microscopia óptica não foi procedimento eficaz na determinação das alterações morfológicas endoteliais estudadas; a microscopia eletrônica mostrou sinais iniciais de sofrimento da célula endotelial e lesão da camada endotelial após 72 horas da ausência de fluxo na artéria normal. Descritores: Endotélio. Microscopia eletrônica. Isquemia. Coelhos 12 -ORIGINAL ARTICLE

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Scanning (SEM) and transmission (TEM) electron-microscopic studies on post-ischemic endothelial lesions following recirculation

Cited by 20 publications

References 12 publications

Release of angiotensin converting enzyme by the lung after Pseudomonas bacteremia in sheep.

Release of angiotensin converting enzyme by the lung after Pseudomonas bacteremia in sheep.

Problems with the preparation of blood vessels for scanning electron microscopy a critical review

Analysis of the effects of the interruption of the flow in the normal arterial endothelium morphology and the correlation with the ischemia duration in rabbits

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