SCAR marker for identification and discrimination of specific medicinal Lycium chinense Miller from Lycium species from ramp-PCR RAPD fragments

Liu, Xiaoyan; Cheng, Jingliang; Mei, Zhiqiang; Wei, Chunli; Peng, Jiangzhou; Fu, Junjiang

doi:10.1007/s13205-020-02325-y

Cited by 16 publications

(5 citation statements)

References 31 publications

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“…In the present study, a specific SCAR marker was developed for the authentication of ‘Daboju,’ based on SCoT analysis. In recent years, SCAR markers have increasingly been used for the authentication of medicinal plants, such as Ocimum tenuiflorum [ 33 ], Trapa natans [ 42 ], Lycium chinense [ 43 ], and Dendrobium officinale [ 36 ]. In addition, SCAR markers have also been used to identify adulterants in commercially important plant-derived food and medicinal products, such as Crocus sativus [ 44 ], Aconitum heterophyllum and Cyperus rotundus [ 37 ], Punica granatum [ 30 ], and Panax ginseng [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Development and Application of a Cultivar-Specific Sequence-Characterized Amplified Region (SCAR) Marker for the Detection of Chrysanthemum morifolium Ramat. ‘Daboju’

Cai

Gao

Zhang

et al. 2022

Plants

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Chrysanthemummorifolium Ramat. ‘Daboju’ is a C. morifolium cultivar with important ornamental and medicinal values, and is often used in the treatment of colds, blurred vision, dizziness, and itchy skin. As the morphological characteristics of C. morifolium ‘Daboju’ are very similar to those of other C. morifolium cultivars, they are often confused in practice. However, the medicinal value and practical use of C. morifolium depends on using the correct rapid and accurate identification of C. morifolium ‘Daboju’ and its differentiation from other, morphologically similar C. × morifolium cultivars. Twenty-one polymorphic start codon-targeted (SCoT) primers were amplified in 21 distinct C. morifolium cultivars. One cultivar-specific DNA marker was developed with the aim of the rapid and accurate identification of C. morifolium ‘Daboju’ and its differentiation from other, similar C. morifolium cultivars. Twenty-one polymorphic start codon-targeted (SCoT) primers were amplified in 21 distinct C. morifolium cultivars. One cultivar-specific 385-bp amplicon (named SCoT36-385), amplified only in C. morifolium ‘Daboju’ (and in all samples of this cultivar), was identified, cloned, and sequenced. Subsequently, a sequence-characterized amplified region (SCAR) marker (named DBJF/DBJR), generating a 360-bp amplicon, was developed from SCoT36-385 and tested for amplification in all 21 C. morifolium cultivars, ten C. morifolium ‘Daboju’ populations, and different simulated adulterations of ‘Daboju’ with other cultivars. The primers amplified the specific 360-bp-long DNA fragment in all the tested C. morifolium ‘Daboju’ samples but failed in the absence of ‘Daboju’. The detection limit of the SCAR primer pair (DBJF/DBJR) was 100 pg of DNA extracted from C. morifolium ‘Daboju’. Hence, this SCAR marker has a very high detection sensitivity, and can be used for accurate and rapid identification of C. morifolium ‘Daboju’. It can play an important role in ensuring the quality of medicinal preparations and protecting C. morifolium ‘Daboju’ germplasm resources in breeding programs and in identifying lines generated from this cultivar.

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Section: Discussionmentioning

confidence: 99%

Development and Application of a Cultivar-Specific Sequence-Characterized Amplified Region (SCAR) Marker for the Detection of Chrysanthemum morifolium Ramat. ‘Daboju’

Cai

Gao

Zhang

et al. 2022

Plants

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“…In 2000, molecular markers such as RAPD were first applied in Lycium species for the validation of the genetic diversity of L. barbarum (Cheng et al, 2000), while RAPD was also validated for its feasibility to distinguish close species of Lycium (Zhang et al, 2001). To date, simple sequence repeat (SSR), conserved ortholog set II (COSII), characterized amplified region (SCAR), amplified fragment length polymorphism (AFLP), restriction site−associated DNA sequencing (RADseq), internal transcribed spacer (ITS), sequence-related amplified polymorphism (SRAP), intersimple sequence repeat (ISSR), and random amplified, microsatellite polymorphism (RAMP)-PCR markers have been applied in genetic diversity, population structure, morphological variation, phylogenetic inference, traceability, and cultivar/species identification and discrimination (Zhang et al, 2001(Zhang et al, , 2018Yin et al, 2005;Sze et al, 2008;Chung et al, 2009;Kwon et al, 2009;Levin et al, 2009;Zhao et al, 2010;Balasubramani et al, 2011;Liu et al, 2012Liu et al, , 2020bTripathi, 2013;Xin et al, 2013;Chen and Zhong, 2014;Wang et al, 2015a;Chen J. et al, 2017;McCulloch et al, 2020;Jung et al, 2021). SSR markers can be used for the detection of genetic polymorphisms of species, calculation of genetic relationships (genetic distance) between varieties, identification of cultivars, and even construction of genetic maps and QTL mapping with sufficient SSR markers Essid et al, 2015;Ibrahim et al, 2016;Thammina et al, 2017;Portis et al, 2018b).…”

Section: Genetic Marker Developmentmentioning

confidence: 99%

Germplasm Resources and Strategy for Genetic Breeding of Lycium Species: A Review

Gong

Rehman

et al. 2022

Front. Plant Sci.

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Lycium species (goji), belonging to Solanaceae, are widely spread in the arid to semiarid environments of Eurasia, Africa, North and South America, among which most species have affinal drug and diet functions, resulting in their potential to be a superior healthy food. However, compared with other crop species, scientific research on breeding Lycium species lags behind. This review systematically introduces the present germplasm resources, cytological examination and molecular-assisted breeding progress in Lycium species. Introduction of the distribution of Lycium species around the world could facilitate germplasm collection for breeding. Karyotypes of different species could provide a feasibility analysis of fertility between species. The introduction of mapping technology has discussed strategies for quantitative trait locus (QTL) mapping in Lycium species according to different kinds of traits. Moreover, to extend the number of traits and standardize the protocols of trait detection, we also provide 1,145 potential traits (275 agronomic and 870 metabolic) in different organs based on different reference studies on Lycium, tomato and other Solanaceae species. Finally, perspectives on goji breeding research are discussed and concluded. This review will provide breeders with new insights into breeding Lycium species.

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“…This supports the applicability of the method for the distinction of different species, and moreover, reveals adulteration within commercial products. Recently, Liu, Cheng, et al (2020) continued the aforementioned studies by developing a novel RAPD-based SCAR marker specific for the medicinal L. chinense Miller in order to separate it from other Lycium species, as well as from its common adulterant species Nitraria sibirica Pall. The newly designed primers were specific to only L. chinense and therefore allowed to distinguish it from the other species.…”

Section: Pcr-based Techniquesmentioning

confidence: 99%

Authentication of berries and berry‐based food products

Salo

Nguyen

Alakärppä

et al. 2021

Comp Rev Food Sci Food Safe

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Berries represent one of the most important and high-valued group of modernday health-beneficial "superfoods" whose dietary consumption has been recognized to be beneficial for human health for a long time. In addition to being delicious, berries are rich in nutrients, vitamins, and several bioactive compounds, including carotenoids, flavonoids, phenolic acids, and hydrolysable tannins. However, due to their high value, berries and berry-based products are often subject to fraudulent adulteration, commonly for economical gain, but also unintentionally due to misidentification of species. Deliberate adulteration often comprises the substitution of high-value berries with lower value counterparts and mislabeling of product contents. As adulteration is deceptive toward customers and presents a risk for public health, food authentication through different methods is applied as a countermeasure. Although many authentication methods have been developed in terms of fast, sensitive, reliable, and low-cost analysis and have been applied in the authentication of a myriad of food products and species, their application on berries and berry-based products is still limited. The present review provides an overview of the development and appli-

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SCAR marker for identification and discrimination of specific medicinal Lycium chinense Miller from Lycium species from ramp-PCR RAPD fragments

Cited by 16 publications

References 31 publications

Development and Application of a Cultivar-Specific Sequence-Characterized Amplified Region (SCAR) Marker for the Detection of Chrysanthemum morifolium Ramat. ‘Daboju’

Development and Application of a Cultivar-Specific Sequence-Characterized Amplified Region (SCAR) Marker for the Detection of Chrysanthemum morifolium Ramat. ‘Daboju’

Germplasm Resources and Strategy for Genetic Breeding of Lycium Species: A Review

Authentication of berries and berry‐based food products

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