SCAR markers for discriminating species of two genera of medicinal plants, Liriope and Ophiopogon

G, Li; Yj, Park

doi:10.4238/2012.may.18.14

Cited by 20 publications

(9 citation statements)

References 21 publications

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“…Species specific markers for plant taxa need to be reliable, reproducible and amplifiable genomic sequences (Das et al 2004). Many reports suggest better reliability of RAPD markers when converted to SCAR (Sun et al 2011;Li and Park 2012). The SCAR marker identified in the present study discriminated K. andamanica from related species in a dominant manner.…”

Section: Discussionmentioning

confidence: 59%

RAPD, SCAR and conserved 18S rDNA markers for a red-listed and endemic medicinal plant species, Knema andamanica (Myristicaceae)

Sheeja

Anju

Shalini

et al. 2013

Physiol Mol Biol Plants

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Knema andamanica is a red-listed endemic medicinal species of Myristicaceae restricted to Andaman and Nicobar (A&N) Islands, India. This species is used in tribal medicines and has immense bioprospective potential. With a view to generate suitable genomic markers for classification and identification, we have generated RAPD, SCAR and conserved 18S rDNA markers from K. andamanica. A unique 585 bp fragment, that distinguished it from seven other related species of Myristicaceae was first amplified using the random primer OPE 06 and converted to SCAR marker (GenBank accession # JN228256). The conserved sequences of 18S rDNA loci from K. andamanica were also amplified and sequenced (GenBank accession #JN228265). The sequence revealed deviations including 18 variable regions and 15 indels that were unique to K. andamanica. These markers can help in definite identification of K. andamanica even at the juvenile stages.

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Section: Discussionmentioning

confidence: 59%

RAPD, SCAR and conserved 18S rDNA markers for a red-listed and endemic medicinal plant species, Knema andamanica (Myristicaceae)

Sheeja

Anju

Shalini

et al. 2013

Physiol Mol Biol Plants

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“…Fu et al (2013) studied Lonicera japonica, a traditionally used medicinal plant by an improved random amplified polymorphic DNA (RAPD) analysis and SCAR markers developed differentiated the different accessions/populations. Similarly Li and Park (2012) while using SCAR markers "SA06 and SB05" differentiated Ophiopogon japonicas with an amplicon of 460 and 553-bp respectively from Liriope platyphylla; the marker SA12 amplified a 485-bp fragment specific to Liriope platyphylla. Another case is the Phyllanthus amarus Schum.…”

Section: Sequence Characterization Of Amplified Regions (Scar)mentioning

confidence: 99%

Authentication of medicinal plants by DNA markers

et al. 2015

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Medicinal plants have been used worldwide for centuries to maintain health and to treat diseases, more so chronic diseases. However, adulteration and use of spurious materials as substitutes have become a major concern for users and industry for reasons of safety and efficacy. Therefore, authentication of medicinal plants is of utmost importance. Morphological, anatomical, chemical and DNA markers solve the problem by differentiating the genuine material from the adulterants, substitutes and spurious drugs. DNA markers use nucleotide sequences to identify species; it takes preference over the other two markers being not age dependent, tissue specific and having a higher discriminating power. Therefore, characterization of plants with such markers is an ideal approach for identification of medicinal plant species and populations/varieties of the same species. Availability of certified taxonomic specimens in herbaria is certainly required for unambiguous confirmation through final visual comparison and analysis.

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“…RAPD-SCAR marker also proved their potency in discriminating species of therapeutically important medicinal plants. Such molecular maker is becoming increasingly popular tools of preferred choice for raw drug authentication and management of herbal drugs [27]. The actinorhizal plant seabuckthorn (Hippophae rhamnoides L., Elaeagnaceae) is a wind pollinated dioecious crop.…”

Section: Applications Of Scar Markermentioning

confidence: 99%

RAPD-SCAR Markers: An Interface Tool for Authentication of Traits

Bhagyawant¹

2016

JBM

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The versatility of the PCR technique is that several kinds of primers can be explored for genome analysis depending on the purpose of study. The easy to access and low cost PCR-based markers include Random Amplified Polymorphic DNA (RAPD). The RAPD markers are easy to develop but lack of reproducibility makes it less reliable and obstacles to their further use in authentication of traits. In addition, other PCR and non PCR based markers like Amplified Fragment Length Polymorphism (AFLP), Simple Sequence Repeat (SSR) and Restriction Fragment Length Polymorphism (RFLP) are also employed in authentication of traits with certain restrictions vis-à-vis use of radioactive materials, high cost and requirement of sequence information etc. Therefore, this problem can be overcome by converting RAPD markers to more robust sequence characterized amplified regions i.e. SCAR markers. SCARs are locus specific, co-dominant in nature and amplified by PCR using specific 15-30 bp DNA fragments. For developing SCAR markers, primers are designed from the nucleotide sequences of a cloned RAPD fragments linked to a trait of interest. SCAR markers are easy to develop and reliable tools for DNA fingerprinting. This mini review is an attempt to summarize efficacy of RAPD-SCAR interface in authentication of traits.

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SCAR markers for discriminating species of two genera of medicinal plants, Liriope and Ophiopogon

Cited by 20 publications

References 21 publications

RAPD, SCAR and conserved 18S rDNA markers for a red-listed and endemic medicinal plant species, Knema andamanica (Myristicaceae)

RAPD, SCAR and conserved 18S rDNA markers for a red-listed and endemic medicinal plant species, Knema andamanica (Myristicaceae)

Authentication of medicinal plants by DNA markers

RAPD-SCAR Markers: An Interface Tool for Authentication of Traits

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