Scattering and absorption measurements of cervical tissues measures using low cost multi-spectral imaging

Bernat, Amir S.; Bar-Am, Kfir; Cataldo, Leigh; Bolton, Frank J.; Kahn, Bruce S.; Levitz, David

doi:10.1117/12.2293816

Cited by 2 publications

(1 citation statement)

References 7 publications

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“…A system like the one described here was deployed in a cervical cancer clinic to look for cervical dysplasia. 43 An exploratory trial has already began.…”

Section: Discussionmentioning

confidence: 99%

Portable, low-cost multispectral imaging system: design, development, validation, and utilization

Bolton

Bernat²,

Bar-Am³

et al. 2018

J. Biomed. Opt.

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Optical spectral images can be used to estimate the amount of bulk absorbers in tissues, specifically oxy-and deoxyhemoglobin, as well as scattering parameters. Most systems that capture spectral image data are large, heavy, and expensive. This paper presents a full end-to-end analysis of a low-cost reflectance-mode multispectral imaging system operating in the visible and near-infrared spectra. The system consists of 13 LEDs mounted on a printed circuit board, a monochrome machine vision camera, and a tablet computer to control the hardware. The bill of materials for the system is less than $1000. Hardware design and implementation are detailed. Calibration, image capture, and preprocessing are also discussed. In validation experiments, excellent agreement is observed in diffuse reflectance measurements between the spectral camera setup and a spectrometer. To demonstrate that such spectral image data can yield meaningful optical measurements in vivo, the forearms of eight volunteers are imaged in the system. Their data are then analyzed to estimate the tissue optical properties of different skin layers using a Monte Carlo lookup table. In three volunteers, spectral images are captured before and after inducing erythema using a warm wet towel. Across the three subjects, a clear increase in the blood content of the superficial plexus layer was observed as a result of the erythema. Collectively, these findings suggest that a low-cost system can capture accurate spectral data and that clinically meaningful information can be derived from it.

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“…A system like the one described here was deployed in a cervical cancer clinic to look for cervical dysplasia. 43 An exploratory trial has already began.…”

Section: Discussionmentioning

confidence: 99%

Portable, low-cost multispectral imaging system: design, development, validation, and utilization

Bolton

Bernat²,

Bar-Am³

et al. 2018

J. Biomed. Opt.

Self Cite

View full text Add to dashboard Cite

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Label‐free assessment of the transformation zone using multispectral diffuse optical imaging toward early detection of cervical cancer

Karthika,

Anantharaju,

Koodi

et al. 2024

Journal of Biophotonics

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The assessment of the transformation zone is a critical step toward diagnosis of cervical cancer. This work involves the development of a portable, label‐free transvaginal multispectral diffuse optical imaging (MDOI) imaging probe to estimate the transformation zone. The images were acquired from N = 5 (N = 1 normal, N = 2 premalignant, and N = 2 malignant) patients. Key parameters such as spectral contrast ratio (ρ) at 545 and 450 nm were higher in premalignant (0.29, 0.25 for 450 nm and 0.30, 0.17 for 545 nm) as compared to the normal patients (0.13 and 0.14 for 450 and 545 nm, respectively). The threshold for the spectral intensity ratio R610/R450 and R610/R545 can also be used as a marker to correlate with the new and original squamous columnar junction (SCJ), respectively. The pilot study highlights the use of new markers such as spectral contrast ratio (ρ) and spectral intensity ratio (R610/R450 and R610/R545) images.

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Scattering and absorption measurements of cervical tissues measures using low cost multi-spectral imaging

Cited by 2 publications

References 7 publications

Portable, low-cost multispectral imaging system: design, development, validation, and utilization

Portable, low-cost multispectral imaging system: design, development, validation, and utilization

Label‐free assessment of the transformation zone using multispectral diffuse optical imaging toward early detection of cervical cancer

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