Schinus terebinthifolius scale-up countercurrent chromatography (Part I): High performance countercurrent chromatography fractionation of triterpene acids with off-line detection using atmospheric pressure chemical ionization mass spectrometry

Vieira, Mariana Neves; Costa, Fernanda das Neves; Leitão, Gilda Guimarães; Garrard, Ian; Hewitson, Peter; Ignatova, Svetlana; Winterhalter, Peter; Jerz, Gerold

doi:10.1016/j.chroma.2015.02.005

Cited by 27 publications

(14 citation statements)

References 21 publications

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“…The solvent system selected was the two-phase solvent system: n -hexane: ethyl acetate: methanol: water 6:5:6:3 v/v/v which provided the better K values for all mayor compounds (0.5 < K < 1.3). A similar solvent system ( n -heptane–ethyl acetate–methanol–water, 6:2:6:2, v/v/v/v) was previously used for the separation of terpenoids (Vieira et al, 2015).…”

Section: Methodsmentioning

confidence: 99%

High resolution UHPLC-MS characterization and isolation of main compounds from the antioxidant medicinal plant Parastrephia lucida (Meyen)

Echiburú-Chau

Pasten

Parra

et al. 2017

Saudi Pharmaceutical Journal

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High-resolution mass spectrometry is currently used to determine the mass of biologically active compounds in medicinal plants and food and UHPLC-Orbitrap is a relatively new technology that allows fast fingerprinting and metabolomics analysis. Forty-two metabolites including several phenolic acids, flavonoids, coumarines, tremetones and ent-clerodane diterpenes were accurately identified for the first time in the resin of the medicinal plant (Asteraceae) a Chilean native species, commonly called umatola, collected in the pre-cordillera and altiplano regions of northern Chile, by means of UHPLC-PDA-HR-MS. This could be possible by the state of the art technology employed, which allowed well resolved total ion current peaks and the proposal of some biosynthetic relationships between the compounds detected. Some mayor compounds were also isolated using HSCCC. The ethanolic extract showed high total polyphenols content and significant antioxidant capacity. Furthermore, several biological assays were performed that determined the high antioxidant capacity found for the mayor compound isolated from the plant, 11--coumaroyloxyltremetone.

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Section: Methodsmentioning

confidence: 99%

High resolution UHPLC-MS characterization and isolation of main compounds from the antioxidant medicinal plant Parastrephia lucida (Meyen)

Echiburú-Chau

Pasten

Parra

et al. 2017

Saudi Pharmaceutical Journal

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“…Off-line HPCCC-ESI-MS/MS profile. The molecular weight profiles of compounds in the HPCCC fractions were obtained via off-line injections to an ion-trap ESI mass spectrometer (HCT-Ultra ETD II, Bruker Daltonics, Bremen, Germany) in the sequence of HPCCC recovery (Vieira et al, 2015). An aliquot volume of 1.5 mL from every second HPCCC fraction (elution and extrusion steps) was pipetted to HPLC vials and directly measured, without further dilution steps.…”

Section: Hpccc Fractionationmentioning

confidence: 99%

“…In addition to UV detection and TLC analysis, off-line molecular weight MS-profiling was used for the evaluation of the HPCCC fractionation of the crude IGF extract (Vieira et al, 2015). Every observed peak corresponded to the injection of one single HPCCC fraction tube carrying the complete molecular weight information of its ionisable compounds.…”

Section: Off-line Hpccc-esi-ms/ms Profilementioning

confidence: 99%

Flavonoids from the flowers of Impatiens glandulifera Royle isolated by high performance countercurrent chromatography

Vieira

Winterhalter

Jerz

2016

Phytochemical Analysis

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“…CCC is a powerful tool in the phytochemical working field as it enables plant extract fractionation with existing major compounds and also isolation or fortification of minor compounds [5,6]. This characteristic is even more noticeable when semi-preparative and preparative scales are employed [7,8]. Standard CCC separation experiments do not provide the full flexibility, solely operating with a two-phase solvent system, and the isocratic elution-extrusion mode [9,10].…”

Section: Introductionmentioning

confidence: 99%

Laguncularia racemosa Phenolics Profiling by Three-Phase Solvent System Step-Gradient Using High-Performance Countercurrent Chromatography with Off-Line Electrospray Mass-Spectrometry Detection

et al. 2021

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The detailed metabolite profiling of Laguncularia racemosa was accomplished by high-performance countercurrent chromatography (HPCCC) using the three-phase system n-hexane–tert-butyl methyl ether–acetonitrile–water 2:3:3:2 (v/v/v/v) in step-gradient elution mode. The gradient elution was adjusted to the chemical complexity of the L. racemosa ethyl acetate partition and strongly improved the polarity range of chromatography. The three-phase solvent system was chosen for the gradient to avoid equilibrium problems when changing mobile phase compositions encountered between the gradient steps. The tentative recognition of metabolites including the identification of novel ones was possible due to the off-line injection of fractions to electrospray ionization mass spectrometry (ESI-MS/MS) in the sequence of recovery. The off-line hyphenation profiling experiment of HPCCC and ESI-MS projected the preparative elution by selected single ion traces in the negative ionization mode. Co-elution effects were monitored and MS/MS fragmentation data of more than 100 substances were used for structural characterization and identification. The metabolite profile in the L. racemosa extract comprised flavonoids, hydrolysable tannins, condensed tannins and low molecular weight polyphenols.

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Schinus terebinthifolius scale-up countercurrent chromatography (Part I): High performance countercurrent chromatography fractionation of triterpene acids with off-line detection using atmospheric pressure chemical ionization mass spectrometry

Cited by 27 publications

References 21 publications

High resolution UHPLC-MS characterization and isolation of main compounds from the antioxidant medicinal plant Parastrephia lucida (Meyen)

High resolution UHPLC-MS characterization and isolation of main compounds from the antioxidant medicinal plant Parastrephia lucida (Meyen)

Flavonoids from the flowers of Impatiens glandulifera Royle isolated by high performance countercurrent chromatography

Laguncularia racemosa Phenolics Profiling by Three-Phase Solvent System Step-Gradient Using High-Performance Countercurrent Chromatography with Off-Line Electrospray Mass-Spectrometry Detection

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