Schisandrin B Inhibits Osteoclastogenesis and Protects Against Ovariectomy-Induced Bone Loss

Abstract

Osteoporosis is a systemic skeletal disease which is highly prevalent worldwide and considered to be associated with excessive bone resorption mediated by osteoclast. Osteoclast differentiation is featured by the activation of inflammation-related pathways and the generation of reactive oxygen species. Schisandrin B is a bioactive compound with strong antiinflammation and antioxidative properties, we thus speculated that Schisandrin B might serve as a potential candidate for osteoporosis. In the present study, we found that the formation and` function of osteoclasts were dramatically suppressed by Schisandrin B. And consistent with the in vitro results, treatment with Schisandrin B attenuated ovariectomy-induced bone loss in mice. Moreover, Schisandrin B notably inhibited the activation of mitogen activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) pathways and scavenged ROS by activating nuclear factor E2 p45-related factor 2 (Nrf2) signaling. In conclusion, our study indicates that Schisandrin B is an effective approach to treat osteoporosis and other osteoclast-related diseases.

Schisandrin B suppresses osteosarcoma lung metastasisin vivoby inhibiting the activation of the Wnt/β‑catenin and PI3K/Akt signaling pathways

“…The characteristics of multi-targeting traditional Chinese medicine extracts with fewer and less severe side-effects in the treatment of tumors has attracted increased attention ( 32 ). Amongst these agents, Sch B has been shown to exert therapeutic effects in a variety of malignant tumors, and recent reports have demonstrated that Sch B may inhibit osteoclast formation and function, highlighting Sch B as a potential drug for OS treatment ( 7 – 11 , 33 ). In the present study, it was revealed that Sch B exerted inhibitory effects on OS in vitro and in vivo via Wnt/β-catenin and PI3K/Akt signaling pathway regulation; SchB inhibited the proliferation, migration and invasion, and promoted the apoptosis of OS cells; it also inhibited the tumor growth and reduced the lung metastasis of OS in vivo .…”

“…Western blotting revealed that Sch B could inhibit OS cell migration and invasion by inhibiting EMT. In addition, it has been demonstrated that Sch B inhibits osteoclastogenesis ( 33 ). In primary bone cancer and metastasis, the process of bone remodeling creates a favorable environment for the establishment and progression of tumors.…”

Herbal Preparation (Bromelain, Papain, Curcuma, Black Pepper) Enhances Mineralization and Reduces Glucocorticoid-Induced Osteoporosis in Zebrafish

“…The discovery of natural extracts or compounds with beneficial effects on osteoporosis is opening a new road toward a sustainable approach to the disease [ 40 ]. Most of them specifically exhibit antioxidant and anti-inflammatory properties, which attenuate the bone-loss phenotype in vivo [ 41 ].…”

Network pharmacology for systematic understanding of Schisandrin B reduces the epithelial cells injury of colitis through regulating pyroptosis by AMPK/Nrf2/NLRP3 inflammasome

“…However, there is no study concerning the specific mechanism of Schisandrin B in attenuating pyroptosis. Meanwhile, there is also no study on Schisandrin B in relieving sepsis-induced colitis by regulating pyroptosis [ 29 , 30 ]. Additionally, Schisandrin B has also been revealed to play a protective role in arthritis [ 31 ].…”