Schnellnachweis von SARS-CoV-2 mit recombinase polymerase amplification

Abstract

The COVID-19 pandemic highlights the need for fast and simple assays for nucleic acid detection. As an isothermal alternative to RT-qPCR, we outline the development of a detection scheme for SARS-CoV-2 RNA based on reverse transcription recombinase polymerase amplification (RT-RPA) technology. RPA uses recombination proteins in combination with a DNA polymerase for rapid amplification of target DNA at a constant temperature (39–42 °C) within 10 to 20 minutes and can be monitored in real-time with fluorescent probes.

Dual Gene Detection of H5N1 Avian Influenza Virus Based on Dual RT-RPA

“…Compared with PCR, RPA detection technology has the advantages of short reaction time, simple operation, high sensitivity and strong specificity [ 31 ]. Although it has only been developed for more than ten years, it has been applied to many fields such as SARS-CoV-2 [ 32 ], parasites [ 33 ] and Vibrio vulnificus [ 34 ]. However, RPA has been developed so far, there is still no special primer design software, and it can only be assisted by PCR primer design software.…”