Personal hygiene is an action taken to maintain one's cleanliness and health for physical and psychological well-being. Factors that influence a person's hygiene are seen from the behavior and conditions of environmental sanitation. This research is a quantitative study using a descriptive observational design. Sampling was done by total sampling technique, with a total sample of 277 people consisting of 124 male students and 153 female students, using Univariate analysis. The results showed that the knowledge, attitudes, and behavior of Santri about personal hygiene in slamic Boarding School of the Metropolitan Ummushabri Kendari were categorized as good, but even though it was categorized as good for Santri actions showed that of 277 respondents (100%) around 17 Santri (6.1%) still had actions lack of personal hygiene, while for the sanitation conditions of the santri's dwelling, the male dormitory and female dormitory are categorized as fulfilling the requirements. With this research, it is expected that efforts in improving personal hygiene and sanitation behavior of each Santri, the results of the study suggest to always conduct socialization or counseling on personal hygiene to all Santri in Islamic Boarding Schools so that Santri can be avoided in various diseases, especially Scabies.