Schwann cells-derived exosomes promote functional recovery after spinal cord injury by promoting angiogenesis

Abstract

Exosomes are small vesicles that contain diverse miRNA, mRNA, and proteins that are secreted by multiple cells, and play a vital function in cell–cell communication. Numerous exosomes produced by cells have been demonstrated to be protective against spinal cord injury (SCI). This study aims to investigate the neuroprotective effect of Schwann cells-derived exosomes (SCs-Exos) on spinal cord injury. We found that SCs-Exos can be taken directly by brain-derived endothelial cells.3 (bEnd.3 cells) and promoted to proliferate, migrate, and form bEnd.3 tube. Additionally, our results showed that the pro-angiogenesis molecules, Integrin-β1, were highly expressed in SCs-Exos. Moreover, we used special shRNA technology to investigate the role of Integrin-β1 in mediating the effect of SCs-Exos-induced angiogenesis on bEnd.3 cells. We observed that the pro-angiogenic effect of SCs-Exos on bEnd.3 cells was suppressed by inhibiting the expression of integrin-β1 in SCs-Exos. In the SCI model, we found that SCs-Exos attenuated tissue damage and improved functional recovery after SCI. Using immunofluorescence staining, we observed that SCs-Exos treatment promoted angiogenesis in SCI, and integrin-β1 was required to promote angiogenesis. In conclusion, our results indicate that SCs-Exos promote angiogenesis by delivering integrin-β1 and may serve as a promising novel therapeutic agent for enhancing neurological functional recovery after SCI.

Heterozygous NF1 dermal fibroblasts modulate exosomal content to promote angiogenesis in a tissue‐engineered skin model of neurofibromatosis type‐1

“…In silico investigation has linked many exosomal proteins, produced by NF1 +/− fibroblasts, to increased angiogenic activity, as well as to enhanced tube formation and microvascular network complexity. Although our data pinpointed dermal fibroblasts as key contributors, it is known that Mast and Schwann cells can also secrete pro‐angiogenic exosomes (Huang et al., 2022; Komi & Redegeld, 2020). Hence, those cell types could also participate in the establishment of a pro‐angiogenic niche for NF1 tumors.…”

“…tube formation and microvascular network complexity. Although our data pinpointed dermal fibroblasts as key contributors, it is known that Mast and Schwann cells can also secrete pro-angiogenic exosomes (Huang et al, 2022;Komi & Redegeld, 2020).…”

Schwann cell‐derived exosomes promote lung cancer progression via miRNA‐21‐5p

“…SC-derived exosomes participate in the regulation of axonal regeneration, angiogenesis, osteogenesis, inflammation, macrophage polarization, and other cell functions (Hao et al, 2023;Huang et al, 2022;Lopez-Verrilli et al, 2013;Ren et al, 2023). However, the role of SC exosomes in tumor measure the size distribution of purified exosomes and revealed a peak at approximately 200 nm (Figure 2a).…”

Non-stem cell-derived exosomes: a novel therapeutics for neurotrauma

“…SCs-Exos have the potential to lessen LPS-induced inflammation in Bone Marrow-derived Macrophages. They achieve this by hindering M1 polarization and encouraging M2 polarization via Milk fat globule-epidermal growth factor-factor 8 (MFG-E8), with the SOCS3/STAT3 signaling pathway playing a role in the process of M2 polarization [ 66 ].On the other hand, Huang et al found in vitro and in vitro experiments that SCs-Exos can be taken up by vascular endothelial cells SCs-Exos promote angiogenesis by delivering integrin-β1 [ 67 ].…”