We study parametric estimation of ergodic diffusions observed at high frequency. Different from the previous studies, we suppose that sampling stepsize is unknown, thereby making the conventional Gaussian quasi-likelihood not directly applicable. In this situation, we construct estimators of both model parameters and sampling stepsize in a fully explicit way, and prove that they are jointly asymptotically normally distributed. High order uniform integrability of the obtained estimator is also derived. Further, we propose the Schwarz (BIC) type statistics for model selection and show its model-selection consistency. We conducted some numerical experiments and found that the observed finite-sample performance well supports our theoretical findings. Also provided is a real data example.Due to the Gaussianity of the driving noise process, this naturally leads to the logarithmic Gaussian quasi-likelihood function (GQLF) based on the small-time Gaussian approximationfor the unknown transition probability distribution. Let us note that the high-frequency setting enables us to develop a unified strategy of parameter estimation for a quite general class of non-linear diffusions. Under appropriate regularity conditions, this quasi-likelihood is known to be theoretically asymptotically efficient. See [7], [11], [23], and the references therein. The existing theoretical literature basically supposes that the unknown quantities h 0 and τ are given a priori, that is, the existing theories have been developed under known h(= τ h 0 ). In practice, the value Date: February 1, 2019.