Sclareol-loaded hyaluronan-coated PLGA nanoparticles: Physico-chemical properties and in vitro anticancer features

Cosco, Donato; Mare, Rosario; Paolino, Donatella; Salvatici, Maria Cristina; Cilurzo, Felisa; Fresta, Massimo

doi:10.1016/j.ijbiomac.2019.03.241

Cited by 40 publications

(29 citation statements)

References 40 publications

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“…The encapsulation of JQ1 did not compromise the aforesaid parameters up to a drug concentration of 0.5 mg/mL, confirming the peculiar properties of PLGA as a useful biomaterial in the development of colloidal systems for the delivery of lipophilic active compounds [15]. The obtained results are in agreement with those previously described by our research team when other lipophilic compounds have been entrapped within PLGA nanoparticles [16][17][18]. The evaluation of backscattering and transmittance profiles of the various systems demonstrated a strong stability of nanoparticles ( Figure 1A).…”

Section: Physico-chemical Characterization Of the Nanosystemssupporting

confidence: 89%

“…PLGA nanoparticles containing JQ1 (0.1-1 mg/mL) were prepared as previously described [16]. Briefly, 2 mL of acetone containing various amounts of drug and PLGA (0.6% w/v) (Sigma Aldrich, Milan, Italy) were mixed with an aqueous solution (5 mL) enriched with poloxamer 188 (1% w/v) (Pluronic ® PE 6800; BASF, Aktiengesellschaft, Ludwigshafen, Germany) by an Ultraturrax (Ultraturrax T25, IKA ® Werke) at 24,000 rpm for 1 min.…”

Section: Preparation and Characterization Of Jq1-loaded Plga Nanopartmentioning

confidence: 99%

“…Briefly, 2 mL of acetone containing various amounts of drug and PLGA (0.6% w/v) (Sigma Aldrich, Milan, Italy) were mixed with an aqueous solution (5 mL) enriched with poloxamer 188 (1% w/v) (Pluronic ® PE 6800; BASF, Aktiengesellschaft, Ludwigshafen, Germany) by an Ultraturrax (Ultraturrax T25, IKA ® Werke) at 24,000 rpm for 1 min. Successively, the suspension was purified and characterized as previously reported [16]. The physico-chemical properties of PLGA nanoparticles were investigated by photon correlation spectroscopy in order to evaluate the mean diameter, size distribution and surface charge [43].…”

Section: Preparation and Characterization Of Jq1-loaded Plga Nanopartmentioning

confidence: 99%

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Nanoparticles Loaded with the BET Inhibitor JQ1 Block the Growth of Triple Negative Breast Cancer Cells In Vitro and In Vivo

Maggisano

Celano

Malivindi

et al. 2019

Cancers

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Inhibition of bromo-and extra-terminal domain (BET) proteins, epigenetic regulators of genes involved in cell viability, has been efficiently tested in preclinical models of triple negative breast cancer (TNBC). However, the use of the selective BET-inhibitor JQ1 on humans is limited by its very short half-life. Herein, we developed, characterized and tested a novel formulation of nanoparticles containing JQ1 (N-JQ1) against TNBC in vitro and in vivo. N-JQ1, prepared using the nanoprecipitation method of preformedpoly-lactid-co-glycolic acid in an aqueous solution containing JQ1 and poloxamer-188 as a stabilizer, presented a high physico-chemical stability. Treatment of MDA-MB 157 and MDA-MB 231 TNBC cells with N-JQ1 determined a significant decrease in cell viability, adhesion and migration. Intra-peritoneal administration (5 days/week for two weeks) of N-JQ1 in nude mice hosting a xenograft TNBC after flank injection of MDA-MB-231 cells determined a great reduction in the growth and vascularity of the neoplasm. Moreover, the treatment resulted in a minimal infiltration of nearby tissues. Finally, the encapsulation of JQ1 in nanoparticles improved the anticancer efficacy of this epigenetic compound against TNBC in vitro and in vivo, opening the way to test it in the treatment of TNBC.

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Section: Physico-chemical Characterization Of the Nanosystemssupporting

confidence: 89%

Section: Preparation and Characterization Of Jq1-loaded Plga Nanopartmentioning

confidence: 99%

Section: Preparation and Characterization Of Jq1-loaded Plga Nanopartmentioning

confidence: 99%

See 1 more Smart Citation

Nanoparticles Loaded with the BET Inhibitor JQ1 Block the Growth of Triple Negative Breast Cancer Cells In Vitro and In Vivo

Maggisano

Celano

Malivindi

et al. 2019

Cancers

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“…MCF-7 (breast cancer cell lines) and MDA-MB-231 cells (human breast adenocarcinoma) were incubated in plastic culture dishes (100 mm × 20 mm) in a water-jacketed CO 2 incubator (Thermo Scientific, Dreieich, Germany) at 37 °C (5% CO 2 ) using a DMEM with glutamine, supplemented with penicillin (100 UI/mL), streptomycin (100 ug/mL), amphotericin B (250 ug/mL) and FBS (10%, v / v ), as previously described [ 34 ]. MTT-assay was used to evaluate cell viability after incubation with DOX in the free form (solubilized in water) or encapsulated in GMO-nanostructures and the investigation was performed as a function of the drug concentrations (0.01–5 μM) and incubation times (24, 48 and 72 h).…”

Section: Methodsmentioning

confidence: 99%

Design and Characterization of Glyceryl Monooleate-Nanostructures Containing Doxorubicin Hydrochloride

Gagliardi

Cosco

Udongo³

et al. 2020

Pharmaceutics

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Glyceryl monooleate (GMO) is one of the most popular amphiphilic lipids, which, in the presence of different amounts of water and a proper amount of stabilizer, can promote the development of well defined, thermodynamically stable nanostructures, called lyotropic liquid crystal dispersions. The aim of this study is based on the design, characterization, and evaluation of the cytotoxicity of lyotropic liquid crystal nanostructures containing a model anticancer drug such as doxorubicin hydrochloride. The drug is efficiently retained by the GMO nanosystems by a remote loading approach. The nanostructures prepared with different non-ionic surfactants (poloxamers and polysorbates) are characterized by different physico-chemical features as a function of several parameters, i.e., serum stability, temperature, and different pH values, as well as the amount of cryoprotectants used to obtain suitable freeze-dried systems. The nanostructures prepared with poloxamer 407 used as a stabilizer show an increased toxicity of the entrapped drug on breast cancer cell lines (MCF-7 and MDA-MB-231) due to their ability to sensitize multidrug-resistant (MDR) tumor cells through the inhibition of specific drug efflux transporters. Moreover, the interaction between the nanostructures and the cells occurs after just a few hours, evidencing a huge cellular uptake of the nanosystems.

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“…Active targeting is achieved by the linkage of specific molecules onto the surfaces of the nanosystems in order to promote their interaction with specific receptors that are over-expressed in the tumor tissue/cells (for example transferrin, folate, hormones, hyaluronic acid, etc.) [62,63].…”

Section: In Vivo Fate Of Nanoparticlesmentioning

confidence: 99%

Antitumor Features of Vegetal Protein-Based Nanotherapeutics

et al. 2020

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The introduction of nanotechnology into pharmaceutical application revolutionized the administration of antitumor drugs through the modulation of their accumulation in specific organs/body compartments, a decrease in their side-effects and their controlled release from innovative systems. The use of plant-derived proteins as innovative, safe and renewable raw materials to be used for the development of polymeric nanoparticles unlocked a new scenario in the drug delivery field. In particular, the reduced size of the colloidal systems combined with the peculiar properties of non-immunogenic polymers favored the characterization and evaluation of the pharmacological activity of the novel nanoformulations. The aim of this review is to describe the physico-chemical properties of nanoparticles composed of vegetal proteins used to retain and deliver anticancer drugs, together with the most important preparation methods and the pharmacological features of these potential nanomedicines.

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Sclareol-loaded hyaluronan-coated PLGA nanoparticles: Physico-chemical properties and in vitro anticancer features

Cited by 40 publications

References 40 publications

Nanoparticles Loaded with the BET Inhibitor JQ1 Block the Growth of Triple Negative Breast Cancer Cells In Vitro and In Vivo

Nanoparticles Loaded with the BET Inhibitor JQ1 Block the Growth of Triple Negative Breast Cancer Cells In Vitro and In Vivo

Design and Characterization of Glyceryl Monooleate-Nanostructures Containing Doxorubicin Hydrochloride

Antitumor Features of Vegetal Protein-Based Nanotherapeutics

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