Sclerotia of the acellular (true) slime mould Fuligo septica as a model to study melanization and anabiosis

Krzywda, Anna; Petelenz, Elżbieta; Michalczyk, Dominika; Płonka, Przemysław M.

doi:10.2478/s11658-007-0047-5

Cited by 14 publications

(12 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ESR characterizations of melanins correspond to a peak signal on the spectra near 3355 gauss. These data are coherent among several fungi regardless of the specific melanin biosynthetic pathway or even if the fungus is pathogenic, including C. neoformans [ 21 ]; Blastomyces dermatitidis [ 22 ], P. brasiliensis [ 23 ], H. capsulatum [ 24 ], S. schenckii [ 25 ] and W. dermatitidis [ 26 ], or not, as in the slime mould Fuligo septic [ 27 ], indicating that, at the molecular level, the structure of paramagnetic center is similar on these melanins.…”

Section: Discussionmentioning

confidence: 99%

Melanin in Fonsecaea pedrosoi: a trap for oxidative radicals

et al. 2010

View full text Add to dashboard Cite

BackgroundThe pathogenic fungus Fonsecaea pedrosoi constitutively produces the pigment melanin, an important virulence factor in fungi. Melanin is incorporated in the cell wall structure and provides chemical and physical protection for the fungus.We evaluated the production of nitric oxide (NO) in macrophages, the oxidative burst and the inducible nitric oxide synthase (i-NOS) activity in interactions between activated murine macrophages and F. pedrosoi. Experiments were carried out with or without tricyclazole (TC) treatment, a selective inhibitor of the dihydroxynaphthalene (DHN)-melanin biosynthesis pathway in F. pedrosoi. The paramagnetisms of melanin and the TC-melanin were analysed by electron spin resonance. The fungal growth responses to H2O2 and to S-nitroso-N-acetylpenicillamine (SNAP), a nitric oxide donor, were also evaluated.ResultsMelanised F. pedrosoi cells were more resistant to both H2O2 and NO. Nitrite was not detected in the supernatant of macrophages incubated with melanised fungal cells. However, i-NOS expression was unaffected by the presence of either untreated control F. pedrosoi or TC-treated F. pedrosoi. In addition, the inhibition of the DHN-melanin pathway by TC improved the oxidative burst capability of the macrophages.ConclusionThe NO-trapping ability of F. pedrosoi melanin is an important mechanism to escape the oxidative burst of macrophages.

show abstract

Section: Discussionmentioning

confidence: 99%

Melanin in Fonsecaea pedrosoi: a trap for oxidative radicals

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Recently, true slime molds became recognized as a good model to study differentiation (Krzywda et al, 2008) and production of secondary metabolites Fig. 2.…”

Section: Discussionmentioning

confidence: 99%

Elemental composition of Physarum compressum Alb. et Schw. sporocarps and their structures cultivated on rabbit dung and agar substrates

Janik

Tylko

Ostachowicz

et al. 2010

Microscopy Res & Technique

View full text Add to dashboard Cite

The elemental composition of spores, peridium walls, and lime nodes of Physarum compressum sporocarps, cultivated on rabbit dung as a natural growing environment for the slime mold and on artificial agar medium, was compared to evaluate differences that may be dependent on substrates. Whole fruiting bodies and samples of both experimental media were extracted with nitric acid or Parr digest bomb, respectively, and analyzed by means of total X-ray reflection fluorescence (TXRF). Electron probe microanalysis (EPMA) of spores, peridium walls, and lime nodes structure was carried out with the scanning electron microscope equipped with energy-dispersive spectrometer. Because of minute sizes and roughness of investigated structures, Monte Carlo simulations were utilized to establish analytical conditions of EPMA. Biological and geological standards were used in the quantification of element concentrations. According to TXRF, the fruiting bodies from agar medium revealed lower concentrations of K, Ca, Cr, Mn, and Fe in relation to fruiting bodies from the dung, reflecting elemental relationships in the experimental media. According to EPMA, the highest Ca concentration was found in the lime nodes followed by the peridium and the spores. Culturing of the slime molds on the rabbit dung indicated higher concentration of Ca in the lime nodes and peridium walls when compared with those obtained from the sporocarps grown on agar media. The opposite relation was found for the spores. The concentration of Na, Mg, P, S, and Cl was generally lower in all structures of the sporocarps harvested from the dung than from the agar medium. K was in higher concentration in analyzed structures from dung than from agar. Different element uptake (except for Ca and K) was revealed by the two methods: TXRF and EPMA.

show abstract

“…Acarine parasites occur in most of the recognized acarine lineages, except for Opilioacarida (Krantz and Walter 2009;Walter and Proctor 2013). Most taxa among parasitic mites are parasites of vertebrates, invertebrates or plants (phytophages).…”

Section: Features Of Various Types Of Ecological Interactionsmentioning

confidence: 99%

Tyrophagus putrescentiae (Sarcoptiformes: Acaridae) in the in vitro cultures of slime molds (Mycetozoa): accident, contamination, or interaction?

et al. 2021

View full text Add to dashboard Cite

Tyrophagus putrescentiae (Schrank), commonly known as the cereal mite, cheese mite, or ham mite, is a cosmopolitan species reported from various environments in the wild, including soil, plant material and vertebrate nests. It has also been recognized as a common pest of food storages, mycological collections as well as plant and invertebrate laboratory cultures. Laboratory observations indicate that T. putrescentiae feeds on a large range of dermatophytes, yeasts and molds. We have observed the interspecific relation between this mite and several species of true slime molds (Mycetozoa) under laboratory conditions, which confirms the very broad spectrum of feeding habits of T. putrescentiae. Mycetozoans were grown in semi-sterile in vitro cultures and fed with oat flour or oat flakes. Tyrophagus putrescentiae displayed affinity to all macroscopically identifiable stages of the life cycle of Fuligo septica (L.) F.H. Wigg, Physarum polycephalum Schwein and the Didymium sp. complex [Didymium iridis (Ditmar) Fr., Didymium nigripes (Link) Fr. and Didymium bahiense Gottsb.]: live, decaying or dead plasmodia, sporangia, aethalia, spores and sclerotia. The relation carrying symptoms of various types of interspecific interaction, is hypothesized to form an evolutionarily young phenomenon, which not only identifies a new aspect of mycetozoal biology, but also presents the cereal mite as a species of high adaptive potential.

show abstract

Sclerotia of the acellular (true) slime mould Fuligo septica as a model to study melanization and anabiosis

Cited by 14 publications

References 20 publications

Melanin in Fonsecaea pedrosoi: a trap for oxidative radicals

Melanin in Fonsecaea pedrosoi: a trap for oxidative radicals

Elemental composition of Physarum compressum Alb. et Schw. sporocarps and their structures cultivated on rabbit dung and agar substrates

Tyrophagus putrescentiae (Sarcoptiformes: Acaridae) in the in vitro cultures of slime molds (Mycetozoa): accident, contamination, or interaction?

Contact Info

Product

Resources

About