Scorpion venom peptide SPVII promotes irradiated cells proliferation and increases the expression of the IL-3 receptor

Qiu, Yifang; Jiang, Liyuan; Wang, Caixia; Wang, Yan; Li, Ting; Xing, Baiqian; Zhou, Meixun; Kong, Tao; Dong, Wei

doi:10.1186/2045-3701-3-28

Cited by 5 publications

(8 citation statements)

References 20 publications

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“…SVPII augments the proliferation of these cells in a concentration-dependent manner (12). The results of the present study demonstrated that the principal component in the SVPII, namely SVP-B5, promotes the proliferation of the M-NFS-60 mouse-derived myelocytic leukemia cell line.…”

Section: Discussionsupporting

confidence: 61%

“…A recent study by our group identified SVP-B5, a novel peptide from the SVPII, which mitigates radiation-induced DNA damage and improves the survival rate through the reactive oxygen species-p16/p21 pathway (10). In another study by our group, hematopoietic growth factor-like effects of SVPII were reported (12). The present study demonstrated that the principal component of SVPII, namely SVP-B5, promotes the proliferation of irradiated hematopoietic cells.…”

Section: Introductionsupporting

confidence: 64%

“…Bone marrow mononuclear cells (BM-MNCs) were isolated aseptically from 8-10 week-old male C57BL/6 mice (18-22 g, n=24; Animal Center of Guangzhou Medical University, Guangzhou, China) as described previously (12,13). Mice were housed in specific-pathogen-free (SPF) conditions at 24-25˚C, with ~70% humidity and a 14/10 h light/dark cycle, with access to food and water ad libitum.…”

Section: Isolation Of Bm-mncsmentioning

confidence: 99%

“…The effect of SVP-B5 on the expression of IL-3R was determined in M-NFS-60 cells by immunofluorescence using a laser scanning confocal microscope (Leica DM1 4000B; Leica Microsystems, Wetzlar, Germany) as described previously (12). The M-NFS-60 cells were treated with different concentrations of SVP-B5 for 24 and 48 h. Cells treated with 10 ng/ml Recombinant Human IL-3 (cat.…”

Section: Expression Of Il-3 Receptor (Il-3r) and Phosphorylation Of Jmentioning

confidence: 99%

“…These scorpion venom polypeptides (SVPs) have been demonstrated to have a diverse array of biological activities with high specificities to their targeted sites, including anti-tumor, anti-epileptic, analgesic and ion channel blocking properties (5)(6)(7)(8)(9). Our group has focused on the SVP and SVPII from the Buthus martensii scorpion venom (10)(11)(12). A recent study by our group identified SVP-B5, a novel peptide from the SVPII, which mitigates radiation-induced DNA damage and improves the survival rate through the reactive oxygen species-p16/p21 pathway (10).…”

Section: Introductionmentioning

confidence: 99%

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SVP‑B5 peptide from Buthus?martensii Karsch scorpion venom exerts hyperproliferative effects on irradiated hematopoietic cells

Wang

Xing

et al. 2017

Exp Ther Med

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Abstract. Previous studies have demonstrated the radioprotective efficacy of scorpion venom peptide, fraction II (SVPII) from the venom of Buthus martensii Karsch. In the present study, the SVP-B5 polypeptide, which is one of the active components of SVPII, was purified using a two-step chromatographic process. SVP-B5 significantly promoted the proliferation of irradiated M-NFS-60 mouse-derived myelocytic leukemia cells. In addition, SVP-B5 effectively and persistently promoted hematopoietic recovery and expansion of hematopoietic cells after irradiation as demonstrated by cobblestone area forming cell and long-term bone marrow culture assays. Treatment of M-NFS-60 cells with SVP-B5 upregulated the expression of interleukin 3 receptor and activated the Janus kinase-2/signal transducer and activator of transcription 5 signaling pathway. In conclusion, the present study demonstrated that SVP-B5 has growth factor-like properties and may be used as a therapeutic modality in the recovery of severe myelosuppression, which is a common side effect of radiotherapy.

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Section: Discussionsupporting

confidence: 61%

Section: Introductionsupporting

confidence: 64%

Section: Isolation Of Bm-mncsmentioning

confidence: 99%

Section: Expression Of Il-3 Receptor (Il-3r) and Phosphorylation Of Jmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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SVP‑B5 peptide from Buthus?martensii Karsch scorpion venom exerts hyperproliferative effects on irradiated hematopoietic cells

Wang

Xing

et al. 2017

Exp Ther Med

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Switch of Steady-State to an Accelerated Granulopoiesis in Response to Androctonus australis hector Venom

et al. 2017

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Although several studies have shown that scorpion venoms cause a systemic inflammatory response syndrome, little is known about the contribution of the hematopoietic organs. The aim of this study was to investigate the effect of Androctonus australis hector venom on the bone marrow and on local inflammatory mediators, in concordance with the systemic inflammatory reaction elicited in mice. The consequences of a direct interaction of venom with murine bone marrow cells were also assessed by in vitro study. Obtained results showed that the early systemic neutrophilia correlated with a rapid granulocyte mobilization. This response was followed by an accelerated granulopoiesis that was supported by TNF-α and IL-6 signals. In vitro data revealed that the venom exerted a proliferative effect on murine hematopoietic cells and stimulated their differentiation towards granulocyte lineage mainly through cytokine secretion. In conclusion, this study indicated that the bone marrow rapidly exerts its activity in response to the experimental envenomation via the granulopoiesis process and inflammatory mediators in concert with the development of a systemic response. The ability of venom to directly switch steady-state granulopoiesis to an accelerated state in vitro could aggravate the disturbance caused by the venom. Better understanding of the mechanisms involved may lead to the emergence of new targets to avoid cell spreading and accumulation by acting on the very early stage of the systemic inflammatory response.

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Effects of Scorpion venom peptide B5 on hematopoietic recovery in irradiated mice and the primary mechanisms

Wang

Zhou

et al. 2015

Sci Rep

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Scorpion venom peptide B5 (SVP-B5) stimulates recovery of hematopoiesis after exposure to radiation. However, its radioprotective effects and mechanisms are still unclear. The aim of this study was to investigate the effects of SVP-B5 on hematopoietic recovery in mice after total body irradiation (TBI) at a dose of 7.5Gy and 6Gy and to explore the possible primary mechanisms. SVP-B5 at a dose of 2.63 μg/kg significantly reduced the mortality rate of mice after TBI (p < 0.05). It showed markedly protective effects against radiation injury. SVP-B5 also significantly increased the number of bone marrow nucleated cells (BMNCs) and increased the colony forming unit (CFU) number in irradiated mice, accelerated the post-irradiation recovery of peripheral blood leukocytes and platelets in mice. SVP-B5 treatment markedly reduced the Reactive Oxygen Species (ROS) levels in BMNCs after TBI, reduced γH2AX levels, and decreased the relative expression levels of p16 and p21 mRNA at day14 (d14) after irradiation. Our study indicated that SVP-B5 could partially mitigate radiation-induced DNA damage, enhance the post-radiation hematopoietic recovery, and improve the survival rate probably through the ROS-p16/p21 pathway.

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Scorpion venom peptide SPVII promotes irradiated cells proliferation and increases the expression of the IL-3 receptor

Cited by 5 publications

References 20 publications

SVP‑B5 peptide from Buthus?martensii Karsch scorpion venom exerts hyperproliferative effects on irradiated hematopoietic cells

SVP‑B5 peptide from Buthus?martensii Karsch scorpion venom exerts hyperproliferative effects on irradiated hematopoietic cells

Switch of Steady-State to an Accelerated Granulopoiesis in Response to Androctonus australis hector Venom

Effects of Scorpion venom peptide B5 on hematopoietic recovery in irradiated mice and the primary mechanisms

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