ASVCP quality assurance guidelines: control of preanalytical and analytical factors for hematology for mammalian and nonmammalian species, hemostasis, and crossmatching in veterinary laboratories

Abstract

: In December 2009, the American Society for Veterinary Clinical Pathology (ASVCP) Quality Assurance and Laboratory Standards committee published the updated and peer‐reviewed ASVCP Quality Assurance Guidelines on the Society's website. These guidelines are intended for use by veterinary diagnostic laboratories and veterinary research laboratories that are not covered by the US Food and Drug Administration Good Laboratory Practice standards (Code of Federal Regulations Title 21, Chapter 58). The guidelines have been divided into 3 reports: (1) general analytical factors for veterinary laboratory performance and comparisons; (2) hematology, hemostasis, and crossmatching; and (3) clinical chemistry, cytology, and urinalysis. This particular report is one of 3 reports and provides recommendations for control of preanalytical and analytical factors related to hematology for mammalian and nonmammalian species, hemostasis testing, and crossmatching and is adapted from sections 1.1 and 2.3 (mammalian hematology), 1.2 and 2.4 (nonmammalian hematology), 1.5 and 2.7 (hemostasis testing), and 1.6 and 2.8 (crossmatching) of the complete guidelines. These guidelines are not intended to be all‐inclusive; rather, they provide minimal guidelines for quality assurance and quality control for veterinary laboratory testing and a basis for laboratories to assess their current practices, determine areas for improvement, and guide continuing professional development and education efforts.

The hematology of captive Bobtail lizards (Tiliqua rugosa): blood counts, light microscopy, cytochemistry, and ultrastructure

“…The HGB concentration was manually corrected by subtracting a correction value on the provided chart, as per the manufacturer recommendations for reptile samples. This analyzer has been validated for nonmammalian species, but no method comparison studies have been performed in Bobtail lizards . The indices MCV, MCH, and MCHC were calculated from manually determined data …”

Influence of cross‐match on posttransfusion packed cell volume in feline packed red blood cell transfusion

“…Coagulated recipient blood was centrifuged and the serum harvested as previously described. 18 The recipient serum was diluted 1:2 in saline. Five hundred microliters of recipient serum solution was added to 500 microliters of the donor RBC solution.…”

Comparison of traditional statistical quality control using commercially available control materials and two patient‐based quality control procedures for the ADVIA 120 Hematology System

“…Quality control (QC) procedures are an important part of overall quality assurance programs to produce accurate and reliable hematologic results . The goal of any statistical control procedure is to achieve a high probability of error detection (Ped) and a low probability of false rejection (Pfr) for detection of error that exceeds the level of error acceptable for a result based on its desired use or interpretation .…”