<scp>C</scp>a<sup>2+</sup>‐calmodulin interacts with <scp>D</scp>d<scp>CAD</scp>‐1 and promotes <scp>D</scp>d<scp>CAD</scp>‐1 transport by contractile vacuoles in <i><scp>D</scp>ictyostelium</i> cells

Sriskanthadevan, Shrivani; Brar, Simuran K.; Manoharan, Kumararaaj; Siu, Chi‐Hung

doi:10.1111/febs.12203

Cited by 21 publications

(17 citation statements)

References 33 publications

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“…Cad1, a Ca 2+ ‐dependent, homophilic cell‐to‐cell adhesion molecule that binds to and enters vacuoles, was identified and verified as a CaMBP by Sriskanthadevan et al . (). Produced as a soluble protein early in development, Cad1 is inserted into the plasma membrane through its association with the vacuoles of the CV system.…”

Section: Osmoregulation and Vacuole Functionmentioning

confidence: 99%

Calmodulin‐mediated events during the life cycle of the amoebozoan Dictyostelium discoideum

et al. 2019

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This review focusses on the functions of intracellular and extracellular calmodulin, its target proteins and their binding proteins during the asexual life cycle of Dictyostelium discoideum. Calmodulin is a primary regulatory protein of calcium signal transduction that functions throughout all stages. During growth, it mediates autophagy, the cell cycle, folic acid chemotaxis, phagocytosis, and other functions. During mitosis, specific calmodulin‐binding proteins translocate to alternative locations. Translocation of at least one cell adhesion protein is calmodulin dependent. When starved, cells undergo calmodulin‐dependent chemotaxis to cyclic AMP generating a multicellular pseudoplasmodium. Calmodulin‐dependent signalling within the slug sets up a defined pattern and polarity that sets the stage for the final events of morphogenesis and cell differentiation. Transected slugs undergo calmodulin‐dependent transdifferentiation to re‐establish the disrupted pattern and polarity. Calmodulin function is critical for stalk cell differentiation but also functions in spore formation, events that begin in the pseudoplasmodium. The asexual life cycle restarts with the calmodulin‐dependent germination of spores. Specific calmodulin‐binding proteins as well as some of their binding partners have been linked to each of these events. The functions of extracellular calmodulin during growth and development are also discussed. This overview brings to the forefront the central role of calmodulin, working through its numerous binding proteins, as a primary downstream regulator of the critical calcium signalling pathways that have been well established in this model eukaryote. This is the first time the function of calmodulin and its target proteins have been documented through the complete life cycle of any eukaryote.

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Section: Osmoregulation and Vacuole Functionmentioning

confidence: 99%

Calmodulin‐mediated events during the life cycle of the amoebozoan Dictyostelium discoideum

et al. 2019

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“…For instance, CVs facilitate the transport of DdCAD-1, a Ca 2+ dependent cell-cell adhesion molecule, to the cell surface [53–55]. DdCAD-1 is synthesized as a soluble protein in the cytoplasm and is then transported to the plasma membrane via CVs [53].…”

Section: Discussionmentioning

confidence: 99%

The C Isoform of Dictyostelium Tetraspanins Localizes to the Contractile Vacuole and Contributes to Resistance against Osmotic Stress

et al. 2016

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Tetraspanins (Tsps) are membrane proteins that are widely expressed in eukaryotic organisms. Only recently, Tsps have started to acquire relevance as potential new drug targets as they contribute, via protein-protein interactions, to numerous pathophysiological processes including infectious diseases and cancer. However, due to a high number of isoforms and functional redundancy, knowledge on specific functions of most Tsps is still scarce. We set out to characterize five previously annotated Tsps, TspA-E, from Dictyostelium discoideum, a model for studying proteins that have human orthologues. Using reverse transcriptase PCRs, we found mRNAs for TspA-E in the multicellular slug stage, whereas vegetative cells expressed only TspA, TspC and, to a lesser extent, TspD. We raised antibodies against TspA, TspC and TspD and detected endogenous TspA, as well as heterologously expressed TspA and TspC by Western blot. N-deglycosylation assays and mutational analyses showed glycosylation of TspA and TspC in vivo. GFP-tagged Tsps co-localized with the proton pump on the contractile vacuole network. Deletion strains of TspC and TspD exibited unaltered growth, adhesion, random motility and development. Yet, tspC− cells showed a defect in coping with hypo-osmotic stress, due to accumulation of contractile vacuoles, but heterologous expression of TspC rescued their phenotype. In conclusion, our data fill a gap in Dictyostelium research and open up the possibility that Tsps in contractile vacuoles of e.g. Trypanosoma may one day constitute a valuable drug target for treating sleeping sickness, one of the most threatening tropical diseases.

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“…), yet it has some unexpected functions as it binds CaM for delivery into the contractile vacuole complex (Sriskanthadevan et al. ). Also in Dictyostelium , TgrB and TgrC surface proteins, dedicated to allo‐recognition during transition from uni‐ to multicellularity (Hirose et al.…”

Section: Specific Conservation and Changes During Evolutionmentioning

confidence: 99%

Evolutionary Cell Biology of Proteins from Protists to Humans and Plants

Plattner

2017

J Eukaryotic Microbiology

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During evolution, the cell as a fine-tuned machine had to undergo permanent adjustments to match changes in its environment, while "closed for repair work" was not possible. Evolution from protists (protozoa and unicellular algae) to multicellular organisms may have occurred in basically two lineages, Unikonta and Bikonta, culminating in mammals and angiosperms (flowering plants), respectively. Unicellular models for unikont evolution are myxamoebae (Dictyostelium) and increasingly also choanoflagellates, whereas for bikonts, ciliates are preferred models. Information accumulating from combined molecular database search and experimental verification allows new insights into evolutionary diversification and maintenance of genes/proteins from protozoa on, eventually with orthologs in bacteria. However, proteins have rarely been followed up systematically for maintenance or change of function or intracellular localization, acquirement of new domains, partial deletion (e.g. of subunits), and refunctionalization, etc. These aspects are discussed in this review, envisaging "evolutionary cell biology." Protozoan heritage is found for most important cellular structures and functions up to humans and flowering plants. Examples discussed include refunctionalization of voltage-dependent Ca 2+

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Ca²⁺‐calmodulin interacts with DdCAD‐1 and promotes DdCAD‐1 transport by contractile vacuoles in Dictyostelium cells

Cited by 21 publications

References 33 publications

Calmodulin‐mediated events during the life cycle of the amoebozoan Dictyostelium discoideum

Calmodulin‐mediated events during the life cycle of the amoebozoan Dictyostelium discoideum

The C Isoform of Dictyostelium Tetraspanins Localizes to the Contractile Vacuole and Contributes to Resistance against Osmotic Stress

Evolutionary Cell Biology of Proteins from Protists to Humans and Plants

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Ca2+‐calmodulin interacts with DdCAD‐1 and promotes DdCAD‐1 transport by contractile vacuoles in Dictyostelium cells

Cited by 21 publications

References 33 publications

Calmodulin‐mediated events during the life cycle of the amoebozoan Dictyostelium discoideum

Calmodulin‐mediated events during the life cycle of the amoebozoan Dictyostelium discoideum

The C Isoform of Dictyostelium Tetraspanins Localizes to the Contractile Vacuole and Contributes to Resistance against Osmotic Stress

Evolutionary Cell Biology of Proteins from Protists to Humans and Plants

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Ca²⁺‐calmodulin interacts with DdCAD‐1 and promotes DdCAD‐1 transport by contractile vacuoles in Dictyostelium cells