<scp>CD</scp>29 is highly expressed on epithelial, myoepithelial, and mesenchymal stromal cells of human salivary glands

Togarrati, PP; Dinglasan, Nuntana; Desai, Shivani; Ryan, WR; Muench, Marcus O.

doi:10.1111/odi.12812

Cited by 28 publications

(25 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The presence of surface markers such as CD29, CD44, and CD90 is commonly used as a criterion to identify human MSCs [27]. Approximately 33–50% of the PG-DC and SMG-DC expressed CD29 (Figure 2A,B), which is also broadly present in human fetal and adult SG epithelial and myoepithelial cells [28]. CD29 is also a putative SG epithelial stem/progenitor cell marker capable of undergoing epithelial differentiation and inducing SG regeneration when transplanted in vivo [29].…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Strategies for Developing Functional Secretory Epithelia from Porcine Salivary Gland Explant Outgrowth Culture Models

et al. 2019

View full text Add to dashboard Cite

Research efforts have been made to develop human salivary gland (SG) secretory epithelia for transplantation in patients with SG hypofunction and dry mouth (xerostomia). However, the limited availability of human biopsies hinders the generation of sufficient cell numbers for epithelia formation and regeneration. Porcine SG have several similarities to their human counterparts, hence could replace human cells in SG modelling studies in vitro. Our study aims to establish porcine SG explant outgrowth models to generate functional secretory epithelia for regeneration purposes to rescue hyposalivation. Cells were isolated and expanded from porcine submandibular and parotid gland explants. Flow cytometry, immunocytochemistry, and gene arrays were performed to assess proliferation, standard mesenchymal stem cell, and putative SG epithelial stem/progenitor cell markers. Epithelial differentiation was induced and different SG-specific markers investigated. Functional assays upon neurostimulation determined α-amylase activity, trans-epithelial electrical resistance, and calcium influx. Primary cells exhibited SG epithelial progenitors and proliferation markers. After differentiation, SG markers were abundantly expressed resembling epithelial lineages (E-cadherin, Krt5, Krt14), and myoepithelial (α-smooth muscle actin) and neuronal (β3-tubulin, Chrm3) compartments. Differentiated cells from submandibular gland explant models displayed significantly greater proliferation, number of epithelial progenitors, amylase activity, and epithelial barrier function when compared to parotid gland models. Intracellular calcium was mobilized upon cholinergic and adrenergic neurostimulation. In summary, this study highlights new strategies to develop secretory epithelia from porcine SG explants, suitable for future proof-of-concept SG regeneration studies, as well as for testing novel muscarinic agonists and other biomolecules for dry mouth.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Recently, in human fetal and adult SG, Togarrati and others [28] observed the wide expression of CD29 in several cellular compartments, including acinar and ductal epithelial, myoepithelial, and mesenchymal stromal cells. Same authors have proposed the use of such CD29+ cells to devise new cellular therapies for SG disorders.…”

Section: Discussionmentioning

confidence: 99%

Strategies for Developing Functional Secretory Epithelia from Porcine Salivary Gland Explant Outgrowth Culture Models

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The development of both salivary and lacrimal glands is a finely tuned process defined as “branching morphogenesis” that relies on the differential expression of cellular surface receptors and extracellular matrix components like proteoglycans. Indeed, the interactions between cell receptors and ECM elements boost the activation of downstream intracellular signaling pathways that induce the differentiation of progenitor cells into acinar lobules and ducts [60].…”

Section: Discussionmentioning

confidence: 99%

Long Non-Coding RNAs Modulate Sjögren’s Syndrome Associated Gene Expression and Are Involved in the Pathogenesis of the Disease

Dolcino

Tinazzi

Vitali

et al. 2019

JCM

View full text Add to dashboard Cite

Primary Sjögren’s syndrome (pSjS) is a chronic systemic autoimmune disorder, primarily affecting exocrine glands; its pathogenesis is still unclear. Long non-coding RNAs (lncRNAs) are thought to play a role in the pathogenesis of autoimmune diseases and a comprehensive analysis of lncRNAs expression in pSjS is still lacking. To this aim, the expression of more than 540,000 human transcripts, including those ascribed to more than 50,000 lncRNAs is profiled at the same time, in a cohort of 16 peripheral blood mononuclear cells PBMCs samples (eight pSjS and eight healthy subjects). A complex network analysis is carried out on the global set of molecular interactions among modulated genes and lncRNAs, leading to the identification of reliable lncRNA-miRNA-gene functional interactions. Taking this approach, a few lncRNAs are identified as targeting highly connected genes in the pSjS transcriptome, since they have a major impact on gene modulation in the disease. Such genes are involved in biological processes and molecular pathways crucial in the pathogenesis of pSjS, including immune response, B cell development and function, inflammation, apoptosis, type I and gamma interferon, epithelial cell adhesion and polarization. The identification of deregulated lncRNAs that modulate genes involved in the typical features of the disease provides insight in disease pathogenesis and opens avenues for the design of novel therapeutic strategies.

show abstract

“…Relevant in the context of sublingual delivery of vaccines or PBP, MSCs have been described in human oral soft tissues including oral mucosa proper, gingiva, periodontal ligament, dental follicle and dental pulp (20,21). In addition, recent studies have reported the presence of numerous MSCs in fetal and adult connective tissue from human major salivary glands, including parotid, sublingual, and submandibular glands (22,23). These oral MSCs show phenotypical and functional resemblance to MSCs isolated from other tissues (20).…”

Section: Introductionmentioning

confidence: 99%

Involvement of Mesenchymal Stem Cells in Oral Mucosal Bacterial Immunotherapy

et al. 2020

View full text Add to dashboard Cite

Recent clinical observations indicate that bacterial vaccines induce cross-protection against infections produced by different microorganisms. MV130, a polyvalent bacterial sublingual preparation designed to prevent recurrent respiratory infectious diseases, reduces the infection rate in patients with recurrent respiratory tract infections. On the other hand, mesenchymal stem cells (MSCs) are key cell components that contribute to the maintenance of tissue homeostasis and exert both immunostimulatory and immunosuppressive functions. Herein, we study the effects of MV130 in human MSC functionality as a potential mechanism that contributes to its clinical benefits. We provide evidence that during MV130 sublingual immunization of mice, resident oral mucosa MSCs can take up MV130 components and their numbers remain unchanged after vaccination, in contrast to granulocytes that are recruited from extramucosal tissues. MSCs treated in vitro with MV130 show an increased viability without affecting their differentiation potential. In the short-term, MSC treatment with MV130 induces higher leukocyte recruitment and T cell expansion. In contrast, once T-cell activation is initiated, MV130 stimulation induces an up-regulated expression of immunosuppressor factors in MSCs. Accordingly, MV130-primed MSCs reduce T lymphocyte proliferation, induce the differentiation of dendritic cells with immunosuppressive features and favor M2-like macrophage polarization, thus counterbalancing the immune response. In addition, MSCs trained with MV130 undergo functional changes, enhancing their immunomodulatory response to a secondary stimulus. Finally, we show that MSCs are able to uptake, process and retain a reservoir of the TLR ligands derived from MV130 digestion which can be subsequently transferred to dendritic cells, an additional feature that also may be associated to trained immunity.

show abstract

CD29 is highly expressed on epithelial, myoepithelial, and mesenchymal stromal cells of human salivary glands

Abstract: CD29 is widely expressed in human salivary glands, and it could serve as a potential biomarker for devising novel cellular therapeutic and diagnostic strategies for salivary gland disorders and malignancies.

Cited by 28 publications

References 45 publications

Strategies for Developing Functional Secretory Epithelia from Porcine Salivary Gland Explant Outgrowth Culture Models

Strategies for Developing Functional Secretory Epithelia from Porcine Salivary Gland Explant Outgrowth Culture Models

Long Non-Coding RNAs Modulate Sjögren’s Syndrome Associated Gene Expression and Are Involved in the Pathogenesis of the Disease

Involvement of Mesenchymal Stem Cells in Oral Mucosal Bacterial Immunotherapy

Contact Info

Product

Resources

About