<scp>Circ_0016760</scp> accelerates non‐small‐cell lung cancer progression through <scp>miR‐646</scp>/<scp>AKT3</scp> signaling in vivo and in vitro

Chen, Shan; Zhou, Long; Ran, Ruizhi; Huang, Jinqi; Yong, Zheng; Xing, Mingzhao; Cai, Yanli

doi:10.1111/1759-7714.14191

Cited by 10 publications

(6 citation statements)

References 36 publications

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“…Genome wide analysis has also demonstrated that circRNAs are evolutionary conserved among species 32 , 33 . In the field of cancer biology, circRNAs have been found to significantly impact tumor cell growth 34 - 36 , proliferation 37 , 38 , invasion 39 - 41 , adhesion 42 , 43 , metastasis 44 - 47 and apoptosis 48 , 49 , suggesting their potential to be used as diagnostic and prognostic biomarkers, as well as therapeutic agents 50 - 52 .…”

Section: Regulation Of Hippo-yap Signaling Axis By Circrnasmentioning

confidence: 99%

Circular RNAs modulate Hippo-YAP signaling: functional mechanisms in cancer

Qadir¹,

Li²,

Yang³

2022

Theranostics

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The Hippo signaling pathway is an evolutionarily conserved network that regulates organ size and tissue homeostasis in mammals. This pathway controls various cell functions, such as growth, proliferation, survival, apoptosis, and stemness by switching 'on' or 'off' its inhibitory and/or transcriptional module, thereby regulating target gene(s) expression. Altered Hippo signaling has been implicated in various forms of cancers. Increasing evidence suggests cross-talk between the Hippo signaling pathway and non-coding RNAs, in particular circular RNAs (circRNAs). In this context, the current review presents the mechanistic interplay between the Hippo pathway and related circRNAs in various forms of cancers, along with the capabilities of these circRNAs to function either as tumor suppressors or oncogenes through miRNA sponging or protein binding mechanisms. Furthermore, we discuss the constraints and limitations in circRNA mechanistic studies while highlighting some outstanding questions regarding the roles of circRNAs associated with the Hippo-YAP pathway in cancer. Finally, we delineate the potential of these circRNAs to be employed as diagnostic and prognostic biomarkers, as well as molecular hotspots for cancer therapy.

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Section: Regulation Of Hippo-yap Signaling Axis By Circrnasmentioning

confidence: 99%

Circular RNAs modulate Hippo-YAP signaling: functional mechanisms in cancer

Qadir¹,

Li²,

Yang³

2022

Theranostics

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“…Earlier or more sensitive biomarker to diagnose CRC is very good for CRC patients (Yang et al 2017). A growing number of studies have shown that circRNAs were significantly dysregulated and had a very important regulatory role in many diseases, such as hepatocellular carcinoma (Chen et al 2021c), cardiovascular disease , non-small-cell lung cancer (Chen et al 2021b), prostate cancer (Mugoni et al 2021) and breast cancer (Wu et al 2021). In our study, we determined the expression of circ_0001821 in CRC tissues and its cell lines, and investigated more deeply the biological function of circ_0001821 played in CRC development.…”

Section: Discussionmentioning

confidence: 99%

High Expression of circ_0001821 Promoted Colorectal Cancer Progression Through miR-600/ISOC1 Axis

Li¹,

Gao²,

Zhao³

et al. 2022

Biochem Genet

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It has been reported that circRNAs play an important regulatory role in the progression of colorectal cancer (CRC). However, the molecular role of circ_0001821 in CRC development is unclear. In this study, we aimed to investigate the regulatory role and molecular mechanisms of circ_0001821 in CRC. Reverse transcription-quantitative PCR and western blot assays were used to detect the expression of circ_0001821, miR-600 and isochorismatase domain containing 1 (ISOC1) in CRC tissues as well as its cell lines. Colony formation assay and EDU assay were used to detect the proliferative capacity of cells. Transwell assay was used to assess cell migration and invasion ability. Flow cytometry was used to analyze cell apoptosis. ELISA was used to measure the glycolytic capacity of cells. Dual-luciferase reporter assay and RNA pull-down assay were used to analyze the relationships between circ_0001821, miR-600 and ISOC1. Animal experimentation was used to validate the functional study of circ_0001821 in vivo. Immunohistochemistry (IHC) of Ki67 staining analysis was conducted to assess tumor growth. Circ_0001821 and ISOC1 were significantly increased in CRC tissues and its cell lines, and miR-600 was significantly decreased in CRC tissues and its cell lines. Silencing circ_0001821 inhibited cell proliferation, migration, invasion and glycolytic capacity, while inducing apoptosis. And it could inhibit tumor growth in vivo. Circ_0001821 could act as a sponge for miR-600 to regulate CRC processes. ISOC1 was identified as a downstream regulator of miR-600, also miR-600 could regulate the expression of ISOC1. In addition, circ_0001821 could regulate ISOC1 expression changes through miR-600. Mechanistically, either miR-600 inhibitor or overexpression of ISOC1 could reverse the effects of knockdown of circ_0001821 on cell biological properties. Circ_0001821 regulated the developmental process of CRC through miR-600/ISOC1 axis.

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“…MiR-mimics, miR-7 mimics, miR-inhibitor, and miR-7 inhibitor were obtained from RiboBio Company (RiboBio, Guangzhou, China). When cell confluence reached about 70%, Lipofectamine 3000 reagent (Sigma Aldrich, USA) was applied to transfect cells according to the supplier’s procedures (Chen et al 2021 ).…”

Section: Methodsmentioning

confidence: 99%

EIF4A3-induced circular RNA SCAP facilitates tumorigenesis and progression of non-small-cell lung cancer via miR-7/SMAD2 signaling

Zhang

2023

Environ Sci Pollut Res

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The eukaryotic translation initiation factor 4A (eIF4A) family determines transcription efficiency by directly binding to precursor RNAs. One member, EIF4A3, modulates the expression of circRNAs. Circular RNA SCAP (circSCAP), a newly found circRNA, has been implicated in atherosclerosis. Yet, how circSCAP regulates cancer development and progression remains understudied. Here, we investigated the function of circSCAP and the molecular mechanism in the tumorigenesis and progression of non-small-cell lung cancer (NSCLC). CircSCAP was upregulated in both NSCLC tissues and cell lines and was mainly located in the cytoplasm. CircSCAP expression was promoted by EIF4A3, which was associated with poor prognosis in patients with NSCLC. CircSCAP sponged miR-7 to upregulate small mothers against decapentaplegic 2 (SMAD2). CircSCAP knockdown undermined cell proliferation, migration, and invasion abilities in NSCLC cell lines (SPCA1 and A549), which was rescued by either inhibiting miR-7 or overexpressing SMAD2. Moreover, circSCAP knockdown upregulated E-cadherin, while downregulating N-cadherin, Vimentin, and MMP9 in SPCA1 and A549 cells, which were abolished by either inhibiting miR-7 or overexpressing SMAD2. Additionally, miR-7 was markedly downregulated, whereas SMAD2 was significantly upregulated in NSCLC tissues. MiR-7 expression was inversely correlated with circSCAP and SMAD2 expression in NSCLC tissues. In conclusion, this study demonstrates that circSCAP is significantly upregulated in NSCLC cell lines and tissues and elucidates that circSCAP facilitates NSCLC progression by sponging miR-7 and upregulating SMAD2. The study provides a novel molecular target for early diagnosis and treatment of NSCLC.

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Circ_0016760 accelerates non‐small‐cell lung cancer progression through miR‐646/AKT3 signaling in vivo and in vitro

Cited by 10 publications

References 36 publications

Circular RNAs modulate Hippo-YAP signaling: functional mechanisms in cancer

Circular RNAs modulate Hippo-YAP signaling: functional mechanisms in cancer

High Expression of circ_0001821 Promoted Colorectal Cancer Progression Through miR-600/ISOC1 Axis

EIF4A3-induced circular RNA SCAP facilitates tumorigenesis and progression of non-small-cell lung cancer via miR-7/SMAD2 signaling

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