<scp>DNA</scp> methyltransferase immunohistochemical expression in odontogenic tumours

Guimarães, Douglas Magno; Antunes, Daniella Moraes; Duarte, Carina Magalhães Esteves; Ferro, Leonardo Borges; Nunes, Fábio Daumas

doi:10.1111/jop.12208

Cited by 11 publications

(14 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the mean nuclear positivity for DNMT1, DNMT3A, DNMT3B, and H3K9ac, were, respectively, 65.07, 82.03, 39.56, and 88.37%, representing the first report of these proteins in a malignant odontogenic tumor. Our results are in agreement with previous studies [ 39 , 40 ], which suggests that epigenetic dysfunctions could play a role in odontogenic lesion development. The increase of H3K9ac in AC suggests that previously suppressed oncogenes could now be transcribed due to DNA access by histone hyperacetylation.…”

Section: Discussionsupporting

confidence: 94%

“…Considering nuclear-cytoplasmic reactions, the authors found that all cases showed more than 25% DNMT1 positive cells, while 13 out of 16 cases showed a DNMT3A cytoplasmic reaction [ 39 ]. Guimarães et al also found nuclear-cytoplasmic positivity for DNMT1 (higher than 50% of cells), DNMT3A (lower than 20%), and DNMT3B (higher than 50%) [ 40 ]. Although we also observed cytoplasmic stains in our samples, we believe these are background than a genuine reaction.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Expression of DNMTs and H3K9ac in Ameloblastoma and Ameloblastic Carcinoma

Amaral‐Silva

Morais

Wagner

et al. 2021

Front. Oral. Health

View full text Add to dashboard Cite

Objectives: DNA methyltransferases (DNMTs) and the histone modification H3K9ac are epigenetic markers. This study aimed to describe the immunohistochemical expression of DNMT1, DNMT3A, DNMT3B, and H3K9ac in the dental follicle (DF), ameloblastoma (AME), and ameloblastic carcinoma (AC), correlating these expressions with the recurrence and aggressive behavior in ameloblastoma.Study Design: Immunohistochemical reactions were performed in 10 human DFs, 38 ameloblastomas, and 6 AC samples. Another 59 ameloblastomas assembled in a tissue microarray were used to compare the immunoexpression with the clinical, radiographic, and histopathological characteristics and the presence of BRAFv600e mutation. Each slide was digitized as a high-resolution image and quantified by Aperio ScanScope Nuclear V9 software. All statistical analyzes were performed using GraphPad Prism statistical software.Results: DNMT3B expression was higher in ameloblastomas than in the DFs, while the AC overexpressed all proteins. The ameloblastomas with BRAFv600e mutation, vestibular/lingual, or vestibular/palatine bone cortical disruption and maxilla involvement showed DNMT1 overexpression, while recurrent cases had high DNMT3B levels.Conclusions: DNA methylation and histone modification might play a role in the development, clinical aggressiveness, and recurrence rates of ameloblastoma, such as the progression to AC. Further investigation about gene methylations in ameloblastomas is needed to better understand its relationship with aggressiveness and recurrence.

show abstract

Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Expression of DNMTs and H3K9ac in Ameloblastoma and Ameloblastic Carcinoma

Amaral‐Silva

Morais

Wagner

et al. 2021

Front. Oral. Health

View full text Add to dashboard Cite

show abstract

“…Changes of DNA methylation patterns have been associated with various benign and malignant tumors. Guimarães et al () studied the immunohistochemical expression of DNMT1, 3A and 3B, and p21, p27, and E‐cadherin in AOTs, and various other OTs. DNMT1, 3A, and 3B were shown to be expressed in the nuclei and/or cytoplasm of all OTs studied.…”

Section: Resultsmentioning

confidence: 99%

Immunoprofile of the adenomatoid odontogenic tumor

Reichart

Philipsen²,

Khongkhunthian

et al. 2016

Oral Diseases

View full text Add to dashboard Cite

This study was focused on the immunohistochemical profile of the adenomatoid odontogenic tumor. A Pub/Medline search revealed a number of immunohistochemical studies including cytokeratin profiles, extracellular matrix proteins, Integrins, ameloblast-associated proteins resorption regulators (RANK, RANKL), p53, PCNA, MDM2 protein, cyclin D1, Ki-67, Bcl-2 metallothionein, metalloproteinases, D56 hepatocyte growth factor, c-met, DNA methyltransferase, podoplanin, TGF-βI, Smad-2/3, Smad-I-5/-8, Smad 4, beta- catenin, calretinin, and clonality. Careful interpretation of the findings indicates that the adenomatoid odontogenic tumor may be more of a hamartomatous than neoplastic nature.

show abstract

“…Up to now, DNA methylation is the most investigated epigenetic change in tumours. However, there are few studies addressing DNA methylation changes in odontogenic tumours (Moreira et al , ; Gomes et al , ; Guimarães et al , ) and to date, no study addressed the relevance of DNA methylation profile of apoptosis‐related genes in ameloblastomas.…”

Section: Discussionmentioning

confidence: 99%