<scp>E3</scp> ubiquitin ligase <i>Os</i><scp>PIE3</scp> destabilises the B‐lectin receptor‐like kinase <scp>PID2</scp> to control blast disease resistance in rice

Wang, Ke; Shen, Li; Chen, Longxin; Tian, Haoran; Chen, Cong; Fu, Yihan; Du, Hongwu; Hu, Zheng Wei; Zhao, Yuanli; Du, Yanxiu; Li, Junzhou; Zhao, Quanzhi; Du, Changqing

doi:10.1111/nph.18637

Cited by 16 publications

(3 citation statements)

References 82 publications

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“…Very recently, OsPID2, a key protein in the resistance of rice to Magnaporthe oryzae strain ZB15, was shown to interact with U-box E3 ubiquitin ligase OsPIE3 (PID2-interacting E3). This interaction leads to the intracellular kinase domain of PID2 entering the nucleus and reducing blast disease resistance (Wang et al, 2023). These findings indicate that ubiquitination is important in maintaining the function of LecRKs.…”

Section: Ubiquitination and Palmitoylation Modificationsmentioning

confidence: 92%

Ligand recognition and signal transduction by lectin receptor-like kinases in plant immunity

Liu

2023

Front. Plant Sci.

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Lectin receptor-like kinases (LecRKs) locate on the cell membrane and play diverse roles in perceiving environmental factors in higher plants. Studies have demonstrated that LecRKs are involved in plant development and response to abiotic and biotic stresses. In this review, we summarize the identified ligands of LecRKs in Arabidopsis, including extracellular purine (eATP), extracellular pyridine (eNAD+), extracellular NAD+ phosphate (eNADP+) and extracellular fatty acids (such as 3-hydroxydecanoic acid). We also discussed the posttranslational modification of these receptors in plant innate immunity and the perspectives of future research on plant LecRKs.

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Section: Ubiquitination and Palmitoylation Modificationsmentioning

confidence: 92%

Ligand recognition and signal transduction by lectin receptor-like kinases in plant immunity

Liu

2023

Front. Plant Sci.

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“…Subcellular localization assays were performed mainly as previously described ( Wang et al 2023a ). The full-length CDSs of GmGDPD2 , GmMyb73 , and GmGA2ox1 were amplified from soybean cultivar Williams82 and subcloned and inserted into the modified pFGC5941 vector.…”

Section: Methodsmentioning

confidence: 99%

The Myb73–GDPD2–GA2ox1 transcriptional regulatory module confers phosphate deficiency tolerance in soybean

Hu,

Cui,

Wang

et al. 2024

The Plant Cell

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Phosphorus is indispensable in agricultural production. An increasing food supply requires more efficient use of phosphate due to limited phosphate resources. However, how crops regulate phosphate efficiency remains largely unknown. Here, we identified a major quantitative trait locus, qPE19, that controls seven low-phosphate (LP)-related traits in soybean (Glycine max) through linkage mapping and genome-wide association studies. We identified the gene responsible for qPE19 as GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE2 (GmGDPD2), and haplotype 5 represents the optimal allele favoring LP tolerance. Overexpression of GmGDPD2 significantly affects hormone signaling and improves root architecture, phosphate efficiency and yield-related traits; conversely, CRISPR/Cas9-edited plants show decreases in these traits. GmMyb73 negatively regulates GmGDPD2 by directly binding to its promoter, thus GmMyb73 negatively regulates LP tolerance. GmGDPD2 physically interacts with GA 2-oxidase 1 (GmGA2ox1) in the plasma membrane, and overexpressing GmGA2ox1 enhances LP-associated traits, similar to GmGDPD2 overexpression. Analysis of double mutants for GmGDPD2 and GmGA2ox1 demonstrated that GmGDPD2 regulates LP tolerance likely by influencing auxin and gibberellin dose-associated cell division in root. These results reveal a regulatory module that plays a major role in regulating LP tolerance in soybean and is expected to be utilized to develop phosphate-efficient varieties to enhance soybean production, particularly in phosphate-deficient soils.

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“…OsAPIP10 plays a dual role in both PTI and ETI by targeting the NLR protein Piz-t and transcription factors VASCULAR PLANT ONE ZINC FINGER 1 (OsVOZ1) and OsVOZ2 for degradation (Park et al, 2016;Wang et al, 2021b). Furthermore, PID2-INTERACTING E3 (OsPIE3), the F-box-type E3 ubiquitin ligase K16 (OsFBK16), and OsCUL3a destabilize the B-lectin receptor-like kinase PYRICULARIA ORYZAE RESISTANCE D2 (OsPID2), the phenylalanine ammonia lyases (OsPALs), and NON-EXPRESSER OF PATHOGENESIS RELATED 1 (OsNPR1), respectively, to attenuate blast resistance (Liu et al, 2017;Wang et al, 2022Wang et al, , 2023.…”

Section: Introductionmentioning

confidence: 99%

OsATL32 ubiquitinates the reactive oxygen species‐producing OsRac5–OsRbohB module to suppress rice immunity

Yan,

Wang,

et al. 2024

JIPB

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Ubiquitination‐mediated protein degradation is integral to plant immunity, with E3 ubiquitin ligases acting as key factors in this process. Here, we report the functions of OsATL32, a plasma membrane‐localized Arabidopsis Tóxicos En Levadura (ATL)‐type E3 ubiquitin ligase, in rice (Oryza sativa) immunity and its associated regulatory network. We found that the expression of OsATL32 is downregulated in both compatible and incompatible interactions between rice and the rice blast fungus Magnaporthe oryzae. The OsATL32 protein level declines in response to infection by a compatible M. oryzae strain or to chitin treatment. OsATL32 negatively regulates rice resistance to blast and bacterial leaf blight diseases, as well as chitin‐triggered immunity. Biochemical and genetic studies revealed that OsATL32 suppresses pathogen‐induced reactive oxygen species (ROS) accumulation by mediating ubiquitination and degradation of the ROS‐producing OsRac5–OsRbohB module, which enhances rice immunity against M. oryzae. The protein phosphatase PHOSPHATASE AND TENSIN HOMOLOG enhances rice blast resistance by dephosphorylating OsATL32 and promoting its degradation, preventing its negative effect on rice immunity. This study provides insights into the molecular mechanism by which the E3 ligase OsATL32 targets a ROS‐producing module to undermine rice immunity.

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E3 ubiquitin ligase OsPIE3 destabilises the B‐lectin receptor‐like kinase PID2 to control blast disease resistance in rice

Cited by 16 publications

References 82 publications

Ligand recognition and signal transduction by lectin receptor-like kinases in plant immunity

Ligand recognition and signal transduction by lectin receptor-like kinases in plant immunity

The Myb73–GDPD2–GA2ox1 transcriptional regulatory module confers phosphate deficiency tolerance in soybean

OsATL32 ubiquitinates the reactive oxygen species‐producing OsRac5–OsRbohB module to suppress rice immunity

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