eEF1A1 promotes colorectal cancer progression and predicts poor prognosis of patients

Abstract

Colorectal cancer (CRC) is a major leading cause of cancer mortality worldwide in which dysregulated protein synthesis plays an etiologic role. The eukaryotic elongation factor 1 A1 (eEF1A1) exerts significant effects on protein synthesis by contributing to peptide chain extension. Whereas its role in CRC remains to be investigated. In this study, we found that the mRNA and protein levels of eEF1A1 were significantly upregulated in CRC cell lines and tissues. Elevated expression of eEF1A1 was correlated with shorter overall survival in 94 CRC patients. The inhibition of proliferation and cell cycle block were observed in CRC cells after eEF1A1 downregulation. Mechanistically, weighted gene correlation network analysis and further Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis suggested that mitogen‐activated protein kinases (MAPKs) signaling pathways were significantly enriched in high‐eEF1A1 expression group, and the levels of phosphorylated p38/JNK/ERK MAPK were dramatically decreased after eEF1A1 downregulation. Overexpression of eEF1A1 in CRC correlated with a poor prognosis. Collectively, this study determined the oncogenic role of eEF1A1 in CRC proliferation and tumorigenesis. eEF1A1 might be a promising therapeutic target and prognostic biomarker in CRC.

MicroRNA PC-3p-2869 Regulates Antler Growth and Inhibits Proliferation and Migration of Human Osteosarcoma and Chondrosarcoma Cells by Targeting CDK8, EEF1A1, and NTN1

“…In this investigation, 18 functional target genes of miR-PC-2869 were confirmed. Among them, CDK8 [ 25 ], COL4A1 [ 26 ], EEF1A1 [ 27 ], GRIK2 [ 28 ], NTN1 [ 29 ], RNF12 [ 30 ], SLC35A2 [ 31 ], SRGAP1 [ 32 ], TRPS1 [ 33 ], and UBR5 [ 34 ] have been identified as drivers of cancer progression in other studies, implying the potential tumor-suppressive role of miR-PC-2869. In addition, specific sites recognized by miR-PC-2869 on 15 target genes are conserved between human and red deer, including CDK8, EEF1A1, and NTN1.…”

“…Thus, it is plausible that the migratory ability of osteosarcoma, chondrosarcoma, and antler cartilage cells markedly declined with the inhibition of EEF1A1. In addition, EEF1A1 has been reported to control cell proliferation and cell cycle via activating MAPK signaling in colorectal cancer or STAT1 signaling in hepatocellular carcinoma [ 16 , 27 ]. Likewise, our findings indicate that the knockdown of EEF1A1 impedes cell proliferation and cyclin D1 expression in osteosarcoma, chondrosarcoma, and antler cartilage cells.…”

Effects of metronidazole on colorectal cancer occurrence and colorectal cancer liver metastases by regulating Fusobacterium nucleatum in mice

“…Inflammation is closely associated with tumors, with the systemic inflammatory response related to tumors considered as one of the significant indicators of tumor progression, and as a result, various serum systemic inflammatory markers can be used for tumor prediction and prognosis 2,3 . However, most CRC patients are diagnosed as advanced and have poor prognosis 4,5 . Moreover, in the late stage of CRC diagnosis, malignant hyperplasia and widespread metastasis have already occurred 6 .…”

“… 2 , 3 However, most CRC patients are diagnosed as advanced and have poor prognosis. 4 , 5 Moreover, in the late stage of CRC diagnosis, malignant hyperplasia and widespread metastasis have already occurred. 6 The liver is the most common site of CRC metastasis, of which 15%–20% of the patients will be candidates for hepatectomy.…”