GLUT1 expression in human papillomavirus‐positive anogenital lesions

Abstract

Background GLUT1, an ubiquitous glucose transporter in the mammalian cells, is upregulated in many tumours, including human papillomavirus (HPV)‐induced head and neck or cervical cancer. Objective To study in anogenital lesions whether or not GLUT1 expression correlates with genomic high‐risk HPV integration, the first step in neoplastic transformation. Methods Forty‐three HPV‐positive biopsies positive for either low‐risk or high‐risk HPV were selected. Paraffin sections adjacent to those tested for the presence of HPV were processed for GLUT1 immunocytochemistry. GLUT1 expression was analysed by two histologists, blinded to HPV type and status and then compared with HPV typing results. Results Two main staining patterns were observed, either staining from the basal to the granular layer or staining of superficial layers only. The first staining pattern corresponded to lesions with high number of episomal HPV‐positive nuclei. Superficial staining was observed in lesions with low number of episomal HPV nuclei or when high‐risk HPV was integrated in the cell genome. Conclusion Our results show that GLUT1 overexpression correlates with the number of episomally infected cells in the lesion, but not with the type (low or high risk) of HPV.

Metabolic reprogramming in cervical cancer and metabolomics perspectives

“…Higher expression of GLUT1 is an independent prognostic factor for the overall survival of HPV16-positive patients, and HPV16-positive patients overexpressing GLUT1 have lower immune cell scores of CD8+ T cells, B cells and Th1 cells [67]. GLUT1 expression correlates with HPV infection [68]. Several pathways may contribute to the regulation of GLUT1 in cervical cancer.…”

Cutaneous incidentaloma revealed by [18F]‐FDG‐PET/CT

“…Recently, digital staining was introduced within the imaging process, which mimics conventional H&E histopathology successfully. 1 Correlations between CLSM imaging and classic histopathology have been studied in detail for healthy human skin and non-melanoma skin cancer with promising results [2][3][4][5][6] ; only one study has evaluated inflammatory diseases. 7 Cutaneous inflammatory diseases can be complex to distinguish clinically and histologically.…”

“…Kariya et al 1 reported that keratinocytes in PET-positive seborrhoeic keratosis showed higher glucose transporter (GLUT) 1 and GLUT3 expressions than those observed in PET-negative seborrhoeic keratosis and normal skin. Samama et al 5 reported a correlation between GLUT1 overexpression and the number of episomally HPV infected cells in the skin. Therefore, aberrant GLUT expression may enhance FDG uptake in such lesions.…”