<scp>HaCaT</scp>
            cells as a model system to study primary cilia in keratinocytes

Blanchard, Gabriela; Pich-Bavastro, Christine; Hohl, Daniel

doi:10.1111/exd.14626

Cited by 3 publications

(1 citation statement)

References 27 publications

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“…Pathways like 'DNA repair pathways full network', 'DNA IRdamage', 'cellular response via atr', and 'cell cycle' correspond to the established role of p53 in the regulation of cell cycle control and DNA reparation [1]. The enrichment of pathways like 'genes related to primary cilium development' and 'ciliary landscape' was unanticipated, but these outcomes may provide a basis for further investigation since altered ciliation is common for patients with atopic dermatitis and psoriasis, and HaCaT cells represent a convenient model for the investigation of ciliation [28].…”

Section: Transcriptomic Differencesmentioning

confidence: 99%

Exploring the Functions of Mutant p53 through TP53 Knockout in HaCaT Keratinocytes

Romashin,

Rusanov,

Arzumanian

et al. 2024

CIMB

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Approximately 50% of tumors carry mutations in TP53; thus, evaluation of the features of mutant p53 is crucial to understanding the mechanisms underlying cell transformation and tumor progression. HaCaT keratinocytes represent a valuable model for research in this area since they are considered normal, although they bear two gain-of-function mutations in TP53. In the present study, transcriptomic and proteomic profiling were employed to examine the functions of mutant p53 and to investigate the impact of its complete abolishment. Our findings indicate that CRISPR-mediated TP53 knockout results in significant changes at the transcriptomic and proteomic levels. The knockout of TP53 significantly increased the migration rate and altered the expression of genes associated with invasion, migration, and EMT but suppressed the epidermal differentiation program. These outcomes suggest that, despite being dysfunctional, p53 may still possess oncosuppressive functions. However, despite being considered normal keratinocytes, HaCaT cells exhibit oncogenic properties.

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Section: Transcriptomic Differencesmentioning

confidence: 99%

Exploring the Functions of Mutant p53 through TP53 Knockout in HaCaT Keratinocytes

Romashin,

Rusanov,

Arzumanian

et al. 2024

CIMB

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HaCaT cells as a model system to study primary cilia in keratinocytes

Blanchard

Pich-Bavastro

Hohl

2022

Experimental Dermatology

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Primary cilium (PC) is a nonmotile microtubule-based organelle found on the apical surface of most mammalian cell types, where it serves as cellular antenna, sensing diverse extracellular signals. PC is implicated in development and tissue homeostasis and possesses mechanosensory, osmosensory, chemosensory, as well as photosensory functions. 1,2 Core of the PC is called ciliary axoneme, composed of nine sets of parallel doublets of microtubules. In nonmitotic cells, the centrosome, composed of mother and daughter centriole, moves to the apical surface of the cell. 3,4 Mother centriole docks into the plasmatic membrane, which allows its transformation into the basal body. 5 Ciliopathies are a rapidly growing group of genetic human diseases characterized by disordered cilium. As cilia can be found on most cell types of the human body, ciliary malfunction has widespread consequences. 6 We recently published the role of ARP-T1 in Bazex-Dupré-Christol syndrome, which could be considered a skin ciliopathy. 7Multiple signalling pathways have been linked to the PC, either as authentic ciliary pathways, such as canonical hedgehog

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The ability of the Lab4 probiotic consortium to impact upon the functionality of serum deprived human keratinocytes in vitro

Thomas,

Kerry-Smith,

Plummer

et al. 2024

Front. Microbiomes

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IntroductionDysfunction of keratinocytes contributes to a weakened skin barrier and impaired wound healing capability. Evidence suggests that probiotic supplementation can lead to improved skin function in vitro and in vivo. The Lab4 probiotic consortium comprises of two strains of Lactobacillus species and two strains of Bifidobacterium species.MethodsUsing serum deprived conditions to impair the functionality of immortalized human HaCaT keratinocytes, this study aimed to assess the impact of metabolites derived from the Lab4 probiotic consortium on keratinocyte function.ResultsA significant improvement in HaCaT metabolic activity and lower apoptotic activity was observed in tandem with a reduction in Caspase-3 gene expression and a lower Bax/Bcl2 ratio following the addition of Lab4. The probiotic also supported barrier integrity which was better maintained with a significant increase in Filaggrin gene expression. In damaged keratinocytes, Lab4 enhanced rates of re-epithelialization, which were associated with significantly increased gene expression of MMP-1 and enhanced secretion of IL-6 and IL-8.DiscussionThese results suggest that the Lab4 probiotic consortium may have the ability to benefit the functionality of skin.

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HaCaT cells as a model system to study primary cilia in keratinocytes

Cited by 3 publications

References 27 publications

Exploring the Functions of Mutant p53 through TP53 Knockout in HaCaT Keratinocytes

Exploring the Functions of Mutant p53 through TP53 Knockout in HaCaT Keratinocytes

HaCaT cells as a model system to study primary cilia in keratinocytes

The ability of the Lab4 probiotic consortium to impact upon the functionality of serum deprived human keratinocytes in vitro

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