HOTAIR, a long noncoding RNA, is a marker of abnormal cell cycle regulation in lung cancer

Abstract

Dysregulation of the cell cycle is a key indicator of tumors, including lung cancer. Recently, the study of cell cycle inhibitors has made great progress in relation to lung cancer. However, the question of what kinds of patients can use cell cycle inhibitors has plagued us. Therefore, seeking an accurate and convenient marker for the abnormal cell cycle in lung cancer is very important. In the present research, we showed that lncRNA HOTAIR is an optimal indicator of cell cycle dysregulation in lung cancer. In the present study, we investigated HOTAIR‐specific expression in lung primary tumor samples by analyzing the TCGA public database and 67 pairs of patients’ tissues collected from our department. Through the TCGA public database KEGG analysis, HOTAIR correlates with the cell cycle pathway. We identified that HOTAIR and its 2 segments, HOTAIR3′ and HOTAIR5′, promote the cell cycle passing through the restriction point during G1‐S phase by regulating the Rb‐E2F pathway and influence non–small‐cell lung cancer cell proliferation, migration and invasion through epithelial‐mesenchymal transition (EMT) and the β‐catenin pathway in vitro and vivo. Finally, we showed that the high expression of HOTAIR was associated with resistance to gefitinib through the dysregulated cell cycle. In conclusion, HOTAIR could be an ideal indicator of cell cycle dysregulation and guide the use of cell cycle inhibitors.

A Methylation-based nomogram for predicting survival in patients with lung adenocarcinoma

“…We also found that DEGs were signi cantly enriched in angiogenesis, which is a core hallmark of advanced cancers, especially in LUAD [18][19][20]. Besides, other signi cant GO terms, such as regulation of cell cycle and regulation of small GTPase mediated signal transduction were also related to cancer progression and chemoresistance reported in many studies [21,22]. As shown in Fig.…”

Long non-coding RNAs in hematological malignancies: translating basic techniques into diagnostic and therapeutic strategies

“…Another study also showed that MALAT1 interacted with a nuclear protein hnRNP C that is functionally involved in G2/M phase [ 106 ]. Other lncRNAs including HOTAIR [ 107 , 108 ], HOXA11-AS [ 40 ], lncRNA-HEIH [ 109 ], MEG3 [ 110 ], ncRNA CCND1 [ 111 ], and PANDA [ 39 , 112 ] are reported with their significant roles in regulating the expression of cell cycle regulators and respective cell cycle stages (Table 3 ).…”

<p>Long Non-Coding RNA USP2-AS1 Accelerates Cell Proliferation and Migration in Ovarian Cancer by Sponging miR-520d-3p and Up-Regulating KIAA1522</p>

“…HOTAIR could be regarded as a potential indicator of cell cycle dysregulation in lung cancer. 10 MEG3 was down-regulated in ovarian cancer cells and elicited tumor inhibitor role in this disease. 11 Besides, lncRNAs have also been reported to affect tumor growth, metastasis, and drug resistance by controlling target gene expression and pathway activation.…”